Siloxane-inorganic chemical crosslinking of hyaluronic acid – based hybrid hydrogels: Structural characterization

Sánchez-Téllez, Daniela Anahí; Rodríguez‐Lorenzo, Luís M.; Téllez-Jurado, Lucía

doi:10.1016/j.carbpol.2019.115590

Cited by 12 publications

(18 citation statements)

References 40 publications

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“…[ 23,24 ] HA is a major component of the extracellular matrix and is known to regulate tissue homeostasis, [ 25 ] cell homing, [ 26 ] and inflammation [ 27 ] by interacting with various cell surface receptors (e.g., CD44, RHAMM, ICAM‐1). [ 28 ] Hydrogels containing silanized HA have recently been reported in the literature; however, the reported systems used additional crosslinkers, such as 1,4‐Butanediol diglycidyl ether [ 29 ] or tetraethoxysilane [ 30 ] to form mechanically stable hydrogels, resulting in preformed hydrogels that cannot be used in the framework of in situ forming systems. In this study, we obtained for the first time in situ forming, silanized HA hydrogels with tunable mechanical and swelling properties by finely controlling the molecular weight (MW) and the degree of substitution (DS).…”

Section: Introductionmentioning

confidence: 99%

In Situ Forming, Silanized Hyaluronic Acid Hydrogels with Fine Control Over Mechanical Properties and In Vivo Degradation for Tissue Engineering Applications

Flegeau

Toquet²,

Réthoré

et al. 2020

Adv Healthcare Materials

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In situ forming hydrogels that can be injected into tissues in a minimally-invasive fashion are appealing as delivery vehicles for tissue engineering applications. Ideally, these hydrogels should have mechanical properties matching those of the host tissue, and a rate of degradation adapted for neo-tissue formation. Here, the development of in situ forming hyaluronic acid hydrogels based on the pH-triggered condensation of silicon alkoxide precursors into siloxanes is reported. Upon solubilization and pH adjustment, the low-viscosity precursor solutions are easily injectable through fine-gauge needles prior to in situ gelation. Tunable mechanical properties (stiffness from 1 to 40 kPa) and associated tunable degradability (from 4 days to more than 3 weeks in vivo) are obtained by varying the degree of silanization (from 4.3% to 57.7%) and molecular weight (120 and 267 kDa) of the hyaluronic acid component. Following cell encapsulation, high cell viability (> 80%) is obtained for at least 7 days. Finally, the in vivo biocompatibility of silanized hyaluronic acid gels is verified in a subcutaneous mouse model and a relationship between the inflammatory response and the crosslink density is observed. Silanized hyaluronic acid hydrogels constitute a tunable hydrogel platform for material-assisted cell therapies and tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

In Situ Forming, Silanized Hyaluronic Acid Hydrogels with Fine Control Over Mechanical Properties and In Vivo Degradation for Tissue Engineering Applications

Flegeau

Toquet²,

Réthoré

et al. 2020

Adv Healthcare Materials

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“…The hydrogel employed for the filling of the scaffolds, for example, was an oral grade sodium hyaluronate (BioIberica, Barcelona, Spain) based gel [27].…”

Section: Methodsmentioning

confidence: 99%

Design of Thermoplastic 3D-Printed Scaffolds for Bone Tissue Engineering: Influence of Parameters of “Hidden” Importance in the Physical Properties of Scaffolds

Mateo

Rodríguez‐Lorenzo

2020

Polymers

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Additive manufacturing (AM) techniques are becoming the approaches of choice for the construction of scaffolds in tissue engineering. However, the development of 3D printing in this field brings unique challenges, which must be accounted for in the design of experiments. The common printing process parameters must be considered as important factors in the design and quality of final 3D-printed products. In this work, we study the influence of some parameters in the design and fabrication of PCL scaffolds, such as the number and orientation of layers, but also others of “hidden” importance, such as the cooling down rate while printing, or the position of the starting point in each layer. These factors can have an important impact oin the final porosity and mechanical performance of the scaffolds. A pure polycaprolactone filament was used. Three different configurations were selected for the design of the internal structure of the scaffolds: a solid one with alternate layers (solid) (0°, 90°), a porous one with 30% infill and alternate layers (ALT) (0°, 90°) and a non-alternated configuration consisting in printing three piled layers before changing the orientation (n-ALT) (0°, 0°, 0°, 90°, 90°, 90°). The nozzle temperature was set to 172 °C for printing and the build plate to 40 °C. Strand diameters of 361 ± 26 µm for room temperature cooling down and of 290 ± 30 µm for forced cooling down, were obtained. A compression elastic modulus of 2.12 ± 0.31 MPa for n-ALT and 8.58 ± 0.14 MPa for ALT scaffolds were obtained. The cooling down rate has been observed as an important parameter for the final characteristics of the scaffold.

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“…Cells were highly viable on these gels [106]. New gelling and crosslinking strategies have emerged as convenient and rapid methods for incorporating advanced functionality into HYH-silica gels [107,108]. Of particular interest is a new approach [107], which was based on the crosslinking of the polysaccharide chains by a 3-D siloxane organic-inorganic matrix using a sol-gel method.…”

Section: Silicamentioning

confidence: 99%

“…New gelling and crosslinking strategies have emerged as convenient and rapid methods for incorporating advanced functionality into HYH–silica gels [ 107 , 108 ]. Of particular interest is a new approach [ 107 ], which was based on the crosslinking of the polysaccharide chains by a 3-D siloxane organic-inorganic matrix using a sol–gel method. Polycondensation and crosslinking reactions, as well as drying conditions, have been optimized for the development of advanced organic-inorganic composites [ 107 ].…”

Section: Hyh–bioceramic and Hyh–bioglass Compositesmentioning

confidence: 99%

“…Of particular interest is a new approach [ 107 ], which was based on the crosslinking of the polysaccharide chains by a 3-D siloxane organic-inorganic matrix using a sol–gel method. Polycondensation and crosslinking reactions, as well as drying conditions, have been optimized for the development of advanced organic-inorganic composites [ 107 ]. Crosslinked HYH–silica nanohybrids with homogeneously distributed silica throughout the HYH matrix were obtained [ 109 ].…”

Section: Hyh–bioceramic and Hyh–bioglass Compositesmentioning

confidence: 99%

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Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications

2021

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Applications of natural hyaluronic acid (HYH) for the fabrication of organic–inorganic composites for biomedical applications are described. Such composites combine unique functional properties of HYH with functional properties of hydroxyapatite, various bioceramics, bioglass, biocements, metal nanoparticles, and quantum dots. Functional properties of advanced composite gels, scaffold materials, cements, particles, films, and coatings are described. Benefiting from the synergy of properties of HYH and inorganic components, advanced composites provide a platform for the development of new drug delivery materials. Many advanced properties of composites are attributed to the ability of HYH to promote biomineralization. Properties of HYH are a key factor for the development of colloidal and electrochemical methods for the fabrication of films and protective coatings for surface modification of biomedical implants and the development of advanced biosensors. Overcoming limitations of traditional materials, HYH is used as a biocompatible capping, dispersing, and structure-directing agent for the synthesis of functional inorganic materials and composites. Gel-forming properties of HYH enable a facile and straightforward approach to the fabrication of antimicrobial materials in different forms. Of particular interest are applications of HYH for the fabrication of biosensors. This review summarizes manufacturing strategies and mechanisms and outlines future trends in the development of functional biocomposites.

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Siloxane-inorganic chemical crosslinking of hyaluronic acid – based hybrid hydrogels: Structural characterization

Cited by 12 publications

References 40 publications

In Situ Forming, Silanized Hyaluronic Acid Hydrogels with Fine Control Over Mechanical Properties and In Vivo Degradation for Tissue Engineering Applications

In Situ Forming, Silanized Hyaluronic Acid Hydrogels with Fine Control Over Mechanical Properties and In Vivo Degradation for Tissue Engineering Applications

Design of Thermoplastic 3D-Printed Scaffolds for Bone Tissue Engineering: Influence of Parameters of “Hidden” Importance in the Physical Properties of Scaffolds

Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications

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