Siloxane-based biocatalytic films and paints for use as reactive coatings

Abstract

We have developed a new methodology for preparing films and paints suitable for use as biocatalytic coatings. The hydrolytic enzymes pronase and α‐chymotrypsin were immobilized by either sol‐gel entrapment or by covalent attachment into a polydimethylsiloxane (PDMS) matrix and cast into thin films or incorporated into an oil‐based paint formulation. All of the coatings retained enzymatic activity and adhered to several different materials. The enzymatic films and paints also exhibited higher thermostability than enzyme free in solution or covalently attached to the outer surface of PDMS. A porous membrane based on a PDMS‐immobilized enzyme was also prepared by an immersion precipitation process. Protein adsorption measurements showed that the enzyme‐containing films and paints adsorbed less protein than enzyme‐free controls, and that protein adsorption decreased with increasing proteolytic activity of the coating. These coatings thus provide the means to apply a stable enzymatic surface to a wide range of materials, and may be generally useful as biocatalytic paints and films. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 475–482, 2001

Trypsin-enabled construction of anti-fouling and self-cleaning polyethersulfone membrane

“…In recent years enzymes have found widespread use in decontamination of waste streams (Lejeune et al, 1998). Kim et al (2001) and Asuri et al (2007) incorporated enzymes into a variety of different matrices to create self-cleaning dense surfaces that resist protein adsorption. These active proteolytic enzymes attached on surfaces could break down protein foulants, and the fragments can be subsequently removed through a simple water flashing operation.…”

Progress of marine biofouling and antifouling technologies

“…Many types of enzymes, such as oxidoreductases, transferases, hydrolase, lyase, isomerase, and ligase, have been reported to have antifouling capabilities [25,58,[97][98][99][100][101][102][103][104][105][106]. From the perspective of enzymatic antifouling technology, biofouling problems are caused by the formation and reproduction of biofilms, and the adhesion of spores and larvae of macroorganisms.…”

Transport and antifouling properties of papain-based antifouling coatings

“…An interesting feature about enzymes is their ready decomposition in the marine environment to CO 2 and H 2 O by the action of microorganisms [28]. Some studies have reported the use of enzymes as antifouling agent in coatings [33,34], even though the great variability in matrix paints, water conditions and enzyme stability make the practical application of these formulations to real situations difficult [28]. Kristensen et al [33] used glucoamylase and hexose to produce an antifouling coating with good efficiency for up 97 days of immersion in the North Sea.…”

“…Kristensen et al [33] used glucoamylase and hexose to produce an antifouling coating with good efficiency for up 97 days of immersion in the North Sea. Kim et al used pronase and α-chymotrypsin together with poly(dimethylsiloxane), reporting a reduction in protein adsorption [34].…”