“…The washed sections were soaked in a solution of periodate and alcohol for 10 min, and then rinsed in distilled water for 10 min and immersed in a colorless alkaline magenta solution for 1 h. After a final rinse with distilled water for 10 min, the slices were dehydrated and mounted on slides for microscopic observation. 24 The preparation was observed and recorded under a digital slide scanner (Pannoramic 250 FLASH, Hungary).…”
Section: Methodsmentioning
confidence: 99%
“…Changes in the colonic mucus layer are closely related to the onset and progression of colitis. 24 Previous studies have shown that the reduction of mucin and cuprocytes in the mouse intestine is a key factor for the develop-ment of colitis. Therefore, we assessed the distribution of the mucus layer and the abundance of cuprocytes in the mouse intestine using PAS staining.…”
Section: Restoration Of Intestinal Barrier Function In B 2 Supplement...mentioning
While there have been advancements in understanding the direct and indirect impact of riboflavin (B2) on intestinal inflammation, the precise mechanisms are still unknown. This study focuses on evaluating the...
“…The washed sections were soaked in a solution of periodate and alcohol for 10 min, and then rinsed in distilled water for 10 min and immersed in a colorless alkaline magenta solution for 1 h. After a final rinse with distilled water for 10 min, the slices were dehydrated and mounted on slides for microscopic observation. 24 The preparation was observed and recorded under a digital slide scanner (Pannoramic 250 FLASH, Hungary).…”
Section: Methodsmentioning
confidence: 99%
“…Changes in the colonic mucus layer are closely related to the onset and progression of colitis. 24 Previous studies have shown that the reduction of mucin and cuprocytes in the mouse intestine is a key factor for the develop-ment of colitis. Therefore, we assessed the distribution of the mucus layer and the abundance of cuprocytes in the mouse intestine using PAS staining.…”
Section: Restoration Of Intestinal Barrier Function In B 2 Supplement...mentioning
While there have been advancements in understanding the direct and indirect impact of riboflavin (B2) on intestinal inflammation, the precise mechanisms are still unknown. This study focuses on evaluating the...
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