Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signaling

Long, Xingyao; Song, Jiajia; Zhao, Xuebo; Yu, Zhuang; Wang, Hongwei; Liu, Xinqi; Suo, Huayi

doi:10.1002/fsn3.1296

Cited by 24 publications

(11 citation statements)

References 43 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous results showed that after an APAP overdose activation of NF-κB-mediated signaling triggers oxidative stress and a pro-inflammatory response, ultimately causing liver damage [ 21 , 30 , 31 ]. To determine whether NF-κB signaling is modulated in the liver by chronic and infratherapeutic APAP administration, nuclear p65-NF-κB and cytosolic IκBα protein levels were analyzed.…”

Section: Resultsmentioning

confidence: 99%

Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress

Brea

Valdecantos

Rada

et al. 2021

Aging

View full text Add to dashboard Cite

The liver exhibits a variety of functions that are well-preserved during aging. However, the cellular hallmarks of aging increase the risk of hepatic alterations and development of chronic liver diseases. Acetaminophen (APAP) is a first choice for relieving mild-to-moderate pain. Most of the knowledge about APAP-mediated hepatotoxicity arises from acute overdose studies due to massive oxidative stress and inflammation, but little is known about its effect in age-related liver inflammation after chronic exposure. Our results show that chronic treatment of wild-type mice on the B6D2JRcc/Hsd genetic background with APAP at an infratherapeutic dose reduces liver alterations during aging without affecting body weight. This intervention attenuates age-induced mild oxidative stress by increasing HO-1, MnSOD and NQO1 protein levels and reducing ERK1/2 and p38 MAPK phosphorylation. More importantly, APAP treatment counteracts the increase in Cd8 + and the reduction in Cd4 + T lymphocytes observed in the liver with age. This response was also found in peripheral blood mononuclear cells. In conclusion, chronic infratherapeutic APAP treatment protects mice from age-related liver alterations by attenuating oxidative stress and inflammation.

show abstract

Section: Resultsmentioning

confidence: 99%

Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress

Brea

Valdecantos

Rada

et al. 2021

Aging

View full text Add to dashboard Cite

show abstract

“…Oxidative stress caused by abnormal expression of these indicators is involved in the pathogenesis of many clinical diseases [29]. GSH is also an active peptide with good antioxidant effect, which can effectively regulate the oxidation balance of the body and inhibit the damage caused by oxidative stress [30]. The results of this study also showed that MDLF can interfere with the abnormality of oxidation-related indexes after H 2 O 2 treatment of cells, and thus, it reduces the cell damage caused by oxidative stress and plays a role in protecting the normal cells.…”

Section: Discussionmentioning

confidence: 99%

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

Fang

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background In this study, Malus doumeri leaf flavonoids (MDLF) were used as the research object to observe their in vitro antioxidant stress ability. Hydrogen peroxide (H2O2) was used to induce oxidative stress in 293 T cells. Methods MTT, flow cytometry, and qPCR were used to verify the effect of MDLF. Results In vitro cell experiments showed that at a concentration of 0–160 μg/mL, MDLF did not affect the normal proliferation of human embryonic kidney 293 T cells (HEK 293 T cells), and MDLF had no cytotoxic effect in this concentration range. It was found that MDLF could maintain the survival of HEK 293 T cells (82.6%) at a high concentration (160 μg/mL). Morphological observation also found that MDLF can inhibit the cell structure imperfection caused by H2O2. It was also observed that MDLF could significantly increase the levels of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) and reduce the level of malondialdehyde (MDA). The results of quantitative polymerase chain reaction (qPCR) showed that MDLF could significantly up-regulate the mRNA expression levels of CAT, SOD, GSH, GSH-Px, B-cell lymphoma-2 (Bcl-2) and downregulate the expression levels of B-cell lymphoma-2 associated x protein (Bax), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa-B (NF-κB) in oxidative stress-injured cells. The HPLC analysis showed that MDLF contained hyperin, isoquercetin, quercitrin, hesperidin, myricetin, baicalin and quercetin. Conclusion From the experimental results, it was observed that MDLF has a strong anti-oxidation ability in vitro, and it can interfere with the oxidative stress damage caused by H2O2 in 293 T cells. Therefore, MDLF is a type of natural substance with good anti-oxidant effect, and it has the potential to interfere with many diseases.

show abstract

“…This evidence indicates that the use of silkworm pupae oil reduces oxidative damage and inflammatory responses in mice [ 69 ]. Silkworm pupae oil also reduced acetaminophen-induced acute liver injury and alcohol-induced hepatotoxicity and oxidative stress in mice by inhibiting the oxidative-stress-mediated NF-κB signalling pathway [ 67 , 68 ]. Silkworm pupae might serve as a potential drug resource for the treatment of gastric ulcers and the prevention of acute liver injury.…”

Section: Pharmacological Functions and Mechanisms Of Silkworm Pupaementioning

confidence: 99%

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Zhou

Duan

et al. 2022

Foods

View full text Add to dashboard Cite

Silkworm pupae are insects that are beneficial to human health, not only for their high nutritional value but, more importantly, for the variety of pharmacological functions they can perform when consumed. Currently, there is a lot of interest in the pharmaceutical applications of silkworm pupae. In recent years, the biological functions of domestic silkworm pupae have gradually been identified and confirmed, especially for their beneficial effects on human health. Studies have found that silkworm pupae have positive effects on liver protection, immune enhancement, antiapoptosis, antitumour, antibacterial, regulation of blood glucose and blood lipids, and lowering of blood pressure. However, the pharmacological mechanisms and systemic safety of silkworm pupae have not been systematically evaluated. In this paper, the nutritional composition of the pupae of the domestic silkworm is first summarised. The pharmacological functions of silkworm pupae and their components are then classified, and their mechanisms of occurrence are described. In addition, we provide a preliminary evaluation of the safety of silkworm pupae, analyse their application prospects, and suggest future directions for further pharmacological function studies. The aim is to generate interest in the promotion of human health through the use of silkworm pupae.

show abstract

Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signaling

Cited by 24 publications

References 43 publications

Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress

Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Contact Info

Product

Resources

About