Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development

Tanaka, Miyu; Fujii, Tsuguru; Mon, Hiroaki; Lee, Jae Man; Kakino, Kohei; Fukumori, Hisayoshi; Ebihara, Takeru; Nagasato, Takumi; Hino, Masato; Tonooka, Yoshino; Moriyama, Takato; Fujita, Ryosuke; Banno, Yutaka; Kusakabe, Takahiro

doi:10.1016/j.ibmb.2022.103737

Cited by 2 publications

(4 citation statements)

References 32 publications

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“…Notably, several internal reproductive organs, including bursa copulatrix, spermatheca, oviductus communis, and accessory glands differentiated from the Ishiwata's germal discs, develop normally in the Spc /+ mutant and ΔCyclin B3 mutant (Figs 1 and 4(D)). Previous studies have indicated that sob is responsible for the development of the bursa copulatrix, and FoxL21 involves in the development of oogenesis and ovariole in the silkworm 16,17 . Here, we report that Cyclin B3 plays a pivotal role in female internal reproductive organogenesis, particularly in the connection between oviductus geminus and oviductus communis.…”

Section: Discussionmentioning

confidence: 66%

“…Previous studies have indicated that sob is responsible for the development of the bursa copulatrix, and FoxL21 involves in the development of oogenesis and ovariole in the silkworm. 16,17 Here, we report that Cyclin B3 plays a pivotal role in female internal reproductive organogenesis, particularly in the connection between oviductus geminus and oviductus communis. Moreover, it decreased fertility in females as half as many eggs were laid compared to Dazao.…”

Section: Homozygous Recessive Lethality Causes Dysfunction Of Cyclin ...mentioning

confidence: 97%

“…The infertility of the silkworm apodal mutant is due to the degradation of the bursa copulatrix in female moths, which is induced by the loss‐of‐function of the sister of odd and bowl ( Bmsob ) 16 . Moreover, ovary differentiation occurs during oogenesis and ovariole development, and requires the forkhead box transcription factor L2, member 1 ( FoxL21 ) in the silkworm 17 . The dsx contributes to the development and growth of male and female genital primordia.…”

Section: Introductionmentioning

confidence: 99%

“…16 Moreover, ovary differentiation occurs during oogenesis and ovariole development, and requires the forkhead box transcription factor L2, member 1 (FoxL21) in the silkworm. 17 The dsx contributes to the development and growth of male and female genital primordia. It is unclear which genes dsx targets in the regulatory network that generates these sexual dimorphisms.…”

Section: Introductionmentioning

confidence: 99%

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Cyclin B3 plays pleiotropic roles in female reproductive organogenesis and early embryogenesis in the silkworm, Bombyx mori

Hu,

Tan,

Luo

et al. 2023

Pest Management Science

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BACKGROUNDReproductive system plays a crucial role in insect survival, reproduction and species specificity. Understanding the molecular mechanisms underlying reproductive organogenesis contributes to improving the efficiency of sterile insect technique marked by an eco‐friendly pest management strategy. Lepidoptera is one of the largest orders of insects, most of which are major pests in agriculture and forestry. Our study aimed to screen the genes responsible for reproductive organogenesis and unravel the mechanism underlying female reproductive organ defects.RESULTSMorphological investigation of female reproductive organs showed a defective connection between oviductus geminus and oviductus communis on the 2nd day of pupa (P2) in Speckled mutant silkworm. RNA_Seq identified a total of 18,049 transcripts that were expressed in the P2 female internal reproductive organs without ovary in Spc/+ compared to +Spc/+Spc. Differential expression analysis identified 312 up‐regulated genes and 221 down‐regulated genes in Spc/+. KEGG analysis identified 44 significantly enriched pathways. The results of qRT‐PCR performed on 33 genes significantly matched the outcomes of the RNA_Seq. Dysfunction of Cyclin B3 resulted in a defective connection of the oviductus communis with the ovariole, dysfunction of oogenesis, and a petite body. Moreover, homozygous recessive lethality of Cyclin B3/Cyclin B3 occurred during early embryogenesis.CONCLUSIONOur results suggested that Cyclin B3 is a pleiotropic functional gene that regulates early embryogenesis, oogenesis, development, and female reproductive organogenesis. These results showed that Cyclin B3 has significant effects on lepidopteran mortality, growth, and reproductive physiology, which might be considered a novel and potentially eco‐friendly target for lepidopteran pest management.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 66%

Section: Homozygous Recessive Lethality Causes Dysfunction Of Cyclin ...mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cyclin B3 plays pleiotropic roles in female reproductive organogenesis and early embryogenesis in the silkworm, Bombyx mori

Hu,

Tan,

Luo

et al. 2023

Pest Management Science

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Efficient DIPA‐CRISPR‐mediated knockout of an eye pigment gene in the white‐backed planthopper, Sogatella furcifera

Zhang,

Gong,

Zhao

et al. 2023

Insect Science

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Although CRISPR/Cas9 has been widely used in insect gene editing, the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small, have hard shells, and/or are difficult to collect and maintain outside of their normal environment. Such is the case with Sogatella furcifera, the white‐backed planthopper (WBPH), a significant pest of Oryza sativa (rice) that oviposits inside rice stems. Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro‐environment of the rice stem. To bypass these issues, we targeted embryos prior to oviposition via direct parental (DIPA)‐CRISPR, in which Cas9 and single‐guide RNAs (sgRNAs) for the WBPH eye pigment gene tryptophan 2,3‐dioxygenase were injected into the hemocoel of adult females. Females at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene‐editing components. An evaluation of the offspring indicated that the highest G0 gene‐edited efficacy (56.7%) occurred in females injected 2 d posteclosion, and that those mutations were heritably transmitted to the G1 generation. This study demonstrates the potential utility of DIPA‐CRISPR for future gene‐editing studies in non‐model insect species and can facilitate the development of novel pest management applications.

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Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development

Cited by 2 publications

References 32 publications

Cyclin B3 plays pleiotropic roles in female reproductive organogenesis and early embryogenesis in the silkworm, Bombyx mori

Cyclin B3 plays pleiotropic roles in female reproductive organogenesis and early embryogenesis in the silkworm, Bombyx mori

Efficient DIPA‐CRISPR‐mediated knockout of an eye pigment gene in the white‐backed planthopper, Sogatella furcifera

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