Silk fibroin nanoparticles and cancer therapy

Iyyanki, Tejaswi; Hubenak, Justin; Zhang, Q.; Mathur, Anshu B.

doi:10.1016/b978-0-323-39080-4.00002-1

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…In the past decade silk fibroin nanoparticles (SFN) have received considerable attention as a drug delivery system (DDS) due their high binding capacity for various drugs, and interesting properties related to controlled drug release, good biocompatibility, and the ability to degrade into harmless products in biological media. , To date, several preparation methods for the preparation of particles from natural silk fibroins (from silkworms, bees, or spiders, among others), ,,,− or recombinant silks have been described. − Apart from the development of the preparation methods, multiple combinations of drugs and SFNs employing different adsorption or encapsulation techniques have been also reported. − Although many past in vitro and in vivo studies evaluating the biocompatibility and biodegradability of SF have been developed involving bulk implants, − few examples of the evaluation of the inflammatory effects or endocytic uptake of SF particles in relation to their size are available. − Thus, there is still an acute lack of information about their distribution in vivo after tissue injection or intravenous administration.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies

Martínez

Aliaga

López-González

et al. 2020

ACS Biomater. Sci. Eng.

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This work aims to provide an effective and novel dual tool for the biodistribution studies of biopolimeric nanoparticles by using modified silk fibroin nanoparticles as a model. This is an indispensable step in the evaluation of the applicability of biopolymeric nanoparticles as drug delivery systems. In this work, we report a new facile method for radiolabeling silk fibroin nanoparticles conjugated to the chelating agent diethylenetriamine pentaacetic acid and tagged with fluorescein isothiocyanate. Nanoparticles were characterized by means of dynamic light scattering, scanning electron microscopy, and infrared and fluorescence spectroscopy. The in vitro studies included stability in biological media and evaluation of the cytotoxicity of the nanoparticles in a cell culture. The in vivo study was focused on a scintigraphic study over 24 h conducted on New Zealand rabbits, after intra-articular injection of [111In]In-nanoparticles containing 8.03 ± 0.42 MBq. Biodistribution of the nanoparticles was also assessed ex vivo by fluorescence microscopy of post mortem biopsied organs. This radiolabeling method was reproducible and robust with high radiolabeling efficiency (∼80%) and high specific activity suitable for in vivo studies. Radiolabeled nanoparticles, having a hydrodynamic radius of 113.2 ± 2.3 nm, a polydispersity index of 0.101 ± 0.015, and a Z-potential of −30.1 ± 2.0 mV, showed an optimum retention in the articular space, without activity clearance up to 24 h post injection. Thus, an easy and robust radiolabeling method has been developed, and its applicability is demonstrated in vitro and in vivo studies, showing its value for future investigation of silk fibroin nanoparticles as versatile and stable (steady) local drug delivery systems for consideration as a therapeutic option, particularly in the treatment of joint disorders.

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Section: Introductionmentioning

confidence: 99%

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies

Martínez

Aliaga

López-González

et al. 2020

ACS Biomater. Sci. Eng.

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“…Hepatocellular carcinoma [187] SF-Thelebolan matrix Soft tissue carcinoma [188] Doxorubicin loaded SF nanoparticles Brain cancer [189] SF-Sodium alginate nanocarriers Colorectal cancer [190] SF-based metastasis model Breast cancer [191] Triptolide-Celastrol-loaded SF nanoparticles Pancreatic cancer [192] Alpha-mangostin loaded SF nanoparticles Colon cancer; Breast cancer [193] SF rods Breast cancer [194] Quercetin loaded SF nanoparticles Breast cancer; Lung metastasis [195] Biliverdin-SF hydrogel Glioma [196] Floxuridine-loaded SF nanospheres Digestive tract cancer; Lung cancer [197] Curcumin-loaded SF nanoparticles Breast cancer [198] Insects 2022, 13, x 18 of 26…”

Section: Sf Hydrogelsmentioning

confidence: 99%

The Contribution of Silk Fibroin in Biomedical Engineering

Lujerdean

Baci

Cucu

et al. 2022

Insects

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Silk fibroin (SF) is a natural protein (biopolymer) extracted from the cocoons of Bombyx mori L. (silkworm). It has many properties of interest in the field of biotechnology, the most important being biodegradability, biocompatibility and robust mechanical strength with high tensile strength. SF is usually dissolved in water-based solvents and can be easily reconstructed into a variety of material formats, including films, mats, hydrogels, and sponges, by various fabrication techniques (spin coating, electrospinning, freeze-drying, and physical or chemical crosslinking). Furthermore, SF is a feasible material used in many biomedical applications, including tissue engineering (3D scaffolds, wounds dressing), cancer therapy (mimicking the tumor microenvironment), controlled drug delivery (SF-based complexes), and bone, eye and skin regeneration. In this review, we describe the structure, composition, general properties, and structure–properties relationship of SF. In addition, the main methods used for ecological extraction and processing of SF that make it a green material are discussed. Lastly, technological advances in the use of SF-based materials are addressed, especially in healthcare applications such as tissue engineering and cancer therapeutics.

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“…To assess the biocompatibility of the 3B/GE/PCL sample, an MTT assay was performed. MTT assays provide a quantitative assessment of the cellular response to the surface of coated substrates [55][56][57]. The results of cell viability illustrated in Figure 7 were determined by the cell counting method [58].…”

Section: Mtt Analysismentioning

confidence: 99%

Surface Modification of 316L SS Implants by Applying Bioglass/Gelatin/Polycaprolactone Composite Coatings for Biomedical Applications

et al. 2020

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In this study, various composites of bioglass/gelatin/polycaprolactone (BG/GE/PCL) were produced and coated on the surface of 316L stainless steel (SS) to improve its bioactivity. X-ray diffractometry (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were utilized to characterize the specimens. The results showed that bioglass particles were distributed uniformly in the coating. By increasing the wt.% of bioglass in the nanocomposite coatings, the surface roughness and adhesion strength increased. The corrosion behavior of GE/PCL (PCL-10 wt.% gelatin coated on 316L SS) and 3BG/GE/PCL (GE/PCL including 3 wt.% bioglass coated on 316L SS) samples were studied in PBS solution. The results demonstrated that 3BG/GE/PCL sample improved the corrosion resistance drastically compared to the GE/PCL specimen. In vitro bioactivity of samples was examined after soaking the specimens for 7, 14 and 28 days in simulated body fluid (SBF). The results showed a significant apatite formation on the surface of 3BG/GE/PCL samples. The cell viability evaluation was performed using 3- (4, 5-dimethylthiazol-2-yl)-2,5 diphenyltetrazoliumbromide (MTT) tests which confirmed the enhanced cell viability on the surface of 3BG/GE/PCL samples. The in vivo behavior of specimens illustrated no toxicity and inflammatory response and was in a good agreement with the results obtained from the in vitro test.

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Silk fibroin nanoparticles and cancer therapy

Cited by 7 publications

References 21 publications

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies

The Contribution of Silk Fibroin in Biomedical Engineering

Surface Modification of 316L SS Implants by Applying Bioglass/Gelatin/Polycaprolactone Composite Coatings for Biomedical Applications

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Silk fibroin nanoparticles and cancer therapy

Cited by 7 publications

References 21 publications

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with 111In: A Dual Tool for Biodistribution and Stability Studies

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with 111In: A Dual Tool for Biodistribution and Stability Studies

The Contribution of Silk Fibroin in Biomedical Engineering

Surface Modification of 316L SS Implants by Applying Bioglass/Gelatin/Polycaprolactone Composite Coatings for Biomedical Applications

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Product

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Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies

Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with ¹¹¹In: A Dual Tool for Biodistribution and Stability Studies