Silk fibroin has a protective effect against high glucose induced apoptosis in HIT‐T15 cells

Park, Junhong; Nam, Yoonho; Park, So‐Young; Kim, Jin‐Kyung; Choe, Nonghoon; Lee, Jae-Young; Oh, Yang-Seok; Suh, Jun-Gyo

doi:10.1002/jbt.20381

Cited by 12 publications

(17 citation statements)

References 27 publications

(22 reference statements)

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“…Physical exercise is generally recommended for the treatment of T2DM because of its benefits on blood glucose control, insulin tissue sensitivity and weight management despite the fact that their exercise capacity is often impaired [61]. Therefore, the possibility that the LOE treatment might affect the physical exercise performance of db/db mice was determined using a treadmill.…”

Section: Discussionmentioning

confidence: 99%

An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice

Lee

Auger

Park³

et al. 2013

PLoS ONE

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Lindera obtusiloba is a medicinal herb traditionally used in Asia for improvement of blood circulation, treatment of inflammation, and prevention of liver damage. A previous study has shown that an ethanolic extract of Lindera obtusiloba stems (LOE) has vasoprotective and antihypertensive effects. The possibility that Lindera obtusiloba improves endothelial function and metabolic parameters in type 2 diabetes mellitus (T2DM) remains to be examined. Therefore, the aim of the present study was to determine the potential of LOE to prevent the development of an endothelial dysfunction, and improve metabolic parameters including hyperglycemia, albuminuria and physical exercise capacity in db/db mice, an experimental model of T2DM. The effect of LOE (100 mg/kg/day by gavage for 8 weeks) on these parameters was compared to that of an oral antidiabetic drug, pioglitazone (30 mg/kg/day by gavage). Reduced blood glucose level, body weight and albumin-creatinine ratio were observed in the group receiving LOE compared to the control db/db group. The LOE treatment improved endothelium-dependent relaxations, abolished endothelium-dependent contractions to acetylcholine in the aorta, and normalized the increased vascular oxidative stress and expression of NADPH oxidase, cyclooxygenases, angiotensin II, angiotensin type 1 receptors and peroxynitrite and the decreased expression of endothelial NO synthase in db/db mice. The angiotensin-converting enzyme (ACE) activity was reduced in the LOE group compared to that in the control db/db group. LOE also inhibited the activity of purified ACE, COX-1 and COX-2 in a dose-dependent manner. In addition, LOE improved physical exercise capacity. Thus, the present findings indicate that LOE has a beneficial effect on the vascular system in db/db mice by improving endothelium-dependent relaxations and vascular oxidative stress most likely by normalizing the angiotensin system, and also on metabolic parameters, and these effects are associated with an enhanced physical exercise capacity.

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Section: Discussionmentioning

confidence: 99%

An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice

Lee

Auger

Park³

et al. 2013

PLoS ONE

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“…It is well known that pancreatic β cells are extremely sensitive to ROS, and ROS are involved in the progression of pancreatic β cell dysfunction [4]. Since SFH was found to decrease ROS formation in a pancreatic β cell line [19], the reduction of ROS by SFH treatment is likely due to elimination of glucose toxicity resulting in protection from the toxic effects of ROS. In the present study, SFH treatment decreased glucose levels and maintained β cell mass.…”

Section: Discussionmentioning

confidence: 99%

Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice

et al. 2012

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Components of silk including silk fibroin have long been used as anti-diabetic remedies in oriental medicine. However, detailed mechanisms underlying these anti-diabetic effects remain unclear. In this study, we examined the anti-diabetic activity of silk fibroin hydrolysate (SFH) in C57BL/KsJ-db/db (db/db) mice, a well-known animal model of non-insulin dependent diabetes mellitus. When the db/db mice were administered SFH in drinking water for 6 weeks, hyperglycemia in the animals gradually disappeared and the level of glycosylated hemoglobin decreased, indicating that SFH plays important role in reducing the symptoms of diabetes. In addition, SFH-treated db/db mice exhibited improved glucose tolerance with increased plasma insulin levels. Immunohistochemical and morphological analyses showed that SFH up-regulated insulin production by increasing pancreatic β cell mass in the mice. In summary, our results suggest that SFH exerts anti-diabetic effects by increasing pancreatic β cell mass in a non-insulin dependent diabetes mellitus mouse model.

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“…Sericin reduces the levels of serum lipids 18) and fibroin has a protective effect against high glucose-induced apoptosis. 19) Low molecular weight peptides from fibroin also stimulate the differentiation of 3T3 cells to adipocytes. 20) Hence in this study, we examined the antiatopic effect of silk peptide from fibroin.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of the Progression of an Atopic Dermatitis-Like Skin Lesion by Silk Peptide and Identification of Functional Peptides

Ikegawa

Sato²,

Lim

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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The efficacy of silk peptide in treatment of atopic dermatitis was examined in a picryl chloride-induced atopic dermatitis model in NC/Nga mice. Silk peptide ameliorated the development of atopic dermatitis by lowering the serum IgE concentration. Treatment of cultured spleen cells with silk peptide reduced IgE production by enhancing the production of IFN-γ and reducing the level of IL-4. The functional peptides in the silk peptide were identified as mixture of GAGA sequences containing peptides by mass spectrometry and in vitro assay. Our findings indicate that silk peptide exerts an effect on atopic dermatitis by modulating the Th1/Th2 balance.

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Silk fibroin has a protective effect against high glucose induced apoptosis in HIT‐T15 cells

Cited by 12 publications

References 27 publications

An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice

An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice

Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice

Amelioration of the Progression of an Atopic Dermatitis-Like Skin Lesion by Silk Peptide and Identification of Functional Peptides

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