Silk fibroin electrospun nanofiber blends with antibiotics and polyvinyl alcohol for burn wound healing

Abstract

Silk fibroin, is a structural and protein‐based biomacromolecule produced from Bombyx mori silkworm. Silk fibroin has been widely being used for biomedical and biomaterial applications, tissue engineering, and drug delivery since a decade. The present study mainly focused to investigate the wound healing efficiency of the developed silk fibroin‐based extracellular electrospun nanofibrous matrix. Electrospun nanofibers were developed by blending the silk fibroin solution with antibiotics (Ciprofloxacin, Amoxicillin, and Nystatin) and polyvinyl alcohol. Results have shown that the developed extracellular matrix has antimicrobial sensitivity activity against the pathogens which causes infection by forming pus at the site of burn wounds. The cell adhesion and cell viability studies have shown a positive effect on L929 fibroblast cell lines. The morphological studies were carried out on SEM. The structural conformations of the developmental matrix were carried out on XRD. The wound healing efficiency of the developed matrix was checked by using a rat model. The results exhibited a dose dependent healing efficiency. The present results demonstrate the developed silk fibroin extracellular matrix promotes cell proliferation and helps in wound healing by controlled release of the antibiotics without losing their chemical properties.

Recent progress of electrospun nanofibers as burning dressings

“…114 Table 1 summarizes the natural and synthetic polymers used in the preparation of electrospun nanober burn dressings. These polymers mainly include natural ones such as chitosan, [115][116][117][118][119][120] gelatin, [121][122][123] silk broin, 118,124,125 sodium alginate, 126,127 and synthetic polymers such as poly(3-caprolactone), [128][129][130][131][132] poly(vinyl alcohol), 119,125,133 poly(ethylene oxide), 120,127,131 poly(vinyl pyrrolidone), 132 polyurethane 126 and polylactic acid. 134 4.1 Natural polymers 4.1.1 Chitosan.…”

“… 124 Yerra et al prepared electrospun nanofibers by blending silk fibroin, polyvinyl alcohol, and antibiotics. 125 The wound therapeutic effect of nanofiber dressing was tested by a rat burn model. The results showed that the dressing had excellent antibacterial ability, and it also promoted the cell viability and cell adhesion of fibroblasts.…”

A Review on Electrospun Poly(amino acid) Nanofibers and Their Applications of Hemostasis and Wound Healing

“…Burns are a major global public health problem, and burn trauma can be caused by heat (flame or scald), freezing, electricity, chemicals, radiation, or friction. Skin repair after burns requires a complex process following trauma, including coagulation, inflammation, cell proliferation, and tissue remodeling [ 145 , 146 ]. Burns can cause damage to the protective skin barrier and lead to pathogen colonization of the burn wound site.…”

Resveratrol-Ampicillin Dual-Drug Loaded Polyvinylpyrrolidone/Polyvinyl Alcohol Biomimic Electrospun Nanofiber Enriched with Collagen for Efficient Burn Wound Repair

“… 11 , 12 One possible solution is the development of advanced burn wound healing materials, which could provide effective wound treatment while lowering the risk of complications. 3 , 13–15 …”