Silk Fibroin/Collagen Blended Membrane Fabricated via a Green Papermaking Method for Potential Guided Bone Regeneration Application: In Vitro and In Vivo Evaluation

Abstract

Guided bone regeneration (GBR) technology is a commonly used surgical procedure for the repair of damaged periodontal tissues. Poor mechanical property and rapid degradation rate are the major reasons for GBR membrane failure in clinical applications. Herein, we applied a green papermaking method to fabricate silk fibroin (SF) membranes blended with collagen and tested their performance. The results showed that the blended SF75 (SF and collagen in a weight ratio of 75:25) membranes are biocompatible with good mechanical properties in the wet condition and appropriate biodegradation rate. MC3T3-E1 osteoblast cell adhesion and proliferation on the membranes were improved by the hybrid biological functions of SF and collagen. Subcutaneous implantation in rats for 9 weeks demonstrated that the membranes induced a less severe inflammatory response. The biodegradation time of the SF75 membranes was appropriate for tissue regeneration. This research, for the first time, reports a blended membrane prepared from silk fibroin and collagen with an ecofriendly method, which shows promise for application in guided bone regeneration.

Advances in Barrier Membranes for Guided Bone Regeneration Techniques

“…Silk, a macromolecule produced by Cocoon ( Bombyx mori ) or Spider ( Nephila clavipes ), can be made into a variety of biomaterials, including membranes, porous scaffolders, gels, suture materials and non-woven webs after purified ( Liu et al, 2013 ; Qi et al, 2017 ). It is worth mentioning that silk fibroin (SF), a structural protein of silk, has high biocompatibility and less foreign body reaction ( Midha et al, 2016 ; Saleem et al, 2020 ; Luo et al, 2021 ). Compared with collagen membrane, SF, as a new biomedical material, has good biocompatibility as well as excellent mechanical properties, controllable degradation and plasticity, which has been widely used in the research of bone tissue engineering.…”

Chitosan/Silk Fibroin Materials for Biomedical Applications—A Review

“…The review paper about the possibility of blending and crosslinking of silk fibroin has been done by Grabska-Zielińska et al [ 32 ]. Special attention has been paid to silk fibroin blends with collagen [ 86 , 87 , 88 , 89 , 90 , 91 , 92 ]. Using silk fibroin and collagen blend thin film and membrane [ 93 ], hydrogels [ 94 ], electrospun fibers [ 95 , 96 ], and hard connective tissue materials [ 97 , 98 , 99 , 100 ] can be prepared.…”

Fabrication of Rechargeable Photoactive Silk Fibroin/Polyvinyl Alcohol Blend Nanofibrous Membranes for Killing Bacteria

“…In fact, some disadvantages of pure SF nanofibrous membranes were reported, such as being brittle, having tedious degradation rate control, and so on [22][23][24][25]. Thus, many silk fibroin-based blended nanofibrous membranes were constructed to enhance its performances in various applications [26][27][28][29]. Polyvinyl alcohol (PVA) deserves special attention for its remarkable characteristics such as hydrophilicity, biodegradability, elasticity, and biocompatibility [30][31][32][33].…”