Silk fibroin-anastrozole loaded prolonged-release biodegradable nanomedicine: A promising drug delivery system for breast cancer therapy

Nasrine, Arfa; Ahmed, Mohammed Gulzar; Narayana, Soumya

doi:10.1016/j.matpr.2022.06.101

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…The carbon-coated grid was used to dry the samples, and they were then stained negatively with a phosphotungstic acid aqueous solution. Once the sample had dried at a 100 kV accelerating voltage, it was examined under a microscope at 10-to-100-fold expansion ( Nasrine et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, neem has a high concentration of minerals, vitamins, and amino acids crucial for wound healing during the proliferation phase ( Subapriya et al, 2005 ). Silk is a naturally occurring protein that is extracted from mulberry silk ( Nasrine et al, 2022 ). The exterior protein sericin, which may trigger an immune reaction when combined with fibroin, is removed ( Altman et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Neem (Azadirachta Indica) and silk fibroin associated hydrogel: Boon for wound healing treatment regimen

Nasrine,

Narayana,

Gulzar Ahmed

et al. 2023

Saudi Pharmaceutical Journal

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neem (Azadirachta Indica) and silk fibroin associated hydrogel: Boon for wound healing treatment regimen

Nasrine,

Narayana,

Gulzar Ahmed

et al. 2023

Saudi Pharmaceutical Journal

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“…Using a UV/visible spectrophotometer with a maximum wavelength of 262 nm, the amount of ANS that was not trapped in the precipitate was calculated. To reduce handling errors, the entire analysis was conducted three times [17,18].…”

Section: Analysis Of Particle Size Zeta Potential Polydispersity Inde...mentioning

confidence: 99%

Silk Fibroin-Based Anastrozole Nanoparticle Loaded in Situ Injectable: Development and Characterization

2023

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Objective: The study aims to find a suitable method of developing silk fibroin-based anastrozole nanoparticles and formulate in situ injectables by loading the optimized nanoparticle formulation for the sustained release treatment of breast cancer. Methods: The nanoparticles were formulated utilizing two different methods, solvent change and precipitation approach using silk fibroin. Prepared nanoparticles characterized in terms of size, zeta potential, polydispersity, and entrapment efficiency. The chosen optimized formulation (SF-ANS-NPs-1) was subsequently analyzed for compatibility investigations by Fourier-transform infrared spectroscopy (FT-IR), thermal analysis, surface morphology, x-ray diffraction, transmission electron spectroscopy, cumulative drug release, and stability studies as per ICH guidelines. Followed by formulating and evaluating in situ injectable gel using pluronic F-127. Results: A particle size of 181.70±1.3 nm was reported by the optimized SF-ANS-NPs-1 formulation. FT-IR and thermal studies confirmed the compatibility of the drug with the polymers, and x-ray diffraction studies indicated crystalline nature. Surface morphology analysis indicated nano-size particle formation. A cumulative drug release (%CDR) of 94.15% was noted at the 168th hour. The results of the stability studies were indicated to be consistent over 90 d. In situ gel formulation showed desired spreadability, sol-gel transition temperature (37±0.5 ℃), viscosity (9.37±1.2 mPa·s), desired acidic pH, and a sustained release for 21 d (98.11%) with three months accelerated stability. Conclusion: The results suggested that the combination of anastrozole with silk fibroin in the form of nanoparticles and in situ gelling systems could be an undoubtedly effective delivery method for prolonging breast cancer therapy.

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“…Silk fibroin has good biocompatibility and biodegradability and has been applied in tissue engineering, drug delivery and gene delivery [ 32 , 33 ]. As a drug delivery system, silk fibroin-coated liposomes can improve the cellular uptake rate of small-molecule drugs [ 34 , 35 ]. Silk fibroin-modified polyethylenimine shows reduced cytotoxicity as a gene delivery vector [ 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Silk Fibroin-Modified Liposome/Gene Editing System Knocks out the PLK1 Gene to Suppress the Growth of Lung Cancer Cells

Pan,

Liu,

Fang

et al. 2023

Pharmaceutics

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Polo-like protein kinase 1 (PLK1) plays a key role in lung cancer cell mitosis. The knockout of PLK1 gene by the CRISPR–Cas9 system can effectively inhibit the proliferation of tumor cells, but there is no suitable vector for in vivo delivery. In this study, CRISPR–Cas9 gene knockout plasmids encoding sgRNA, Cas9 and green fluorescent protein were constructed. Then, the plasmids were packaged with liposome (Lip) and cholesterol-modified Antheraea pernyi silk fibroin (CASF) to obtain the CASF/Lip/pDNA ternary complex. The CASF/Lip/pDNA complex was transfected into lung cancer cells A549 to investigate the transfection efficiency, the PLK1 gene knockout effect and the inhibitory effect on lung cancer cells. The results showed that the transfection efficiency of the CASF/Lip/pDNA complex was significantly higher than that of the Lip/pDNA binary complex, and the expression of PLK1 in cells transfected with CASF/Lip/pDNA complexes was significantly lower than that in cells transfected with Lip/pDNA complexes. The CASF/Lip/pDNA complex significantly increased the apoptosis rate and decreased the proliferation activity of lung cancer cells compared with Lip/pDNA complexes. The cytotoxicity of the complexes was evaluated by coculture with the human bronchial epithelial cells BEAS2B. The results showed that CASF/Lip/pDNA complexes exhibited lower cytotoxicity than Lip/pDNA complexes. The fibroin-modified liposome/PLK1 gene knockout system not only effectively inhibited the growth of lung cancer cells but also showed no obvious toxicity to normal cells, showing potential for clinical application in lung cancer therapy.

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Silk fibroin-anastrozole loaded prolonged-release biodegradable nanomedicine: A promising drug delivery system for breast cancer therapy

Cited by 6 publications

References 38 publications

Neem (Azadirachta Indica) and silk fibroin associated hydrogel: Boon for wound healing treatment regimen

Neem (Azadirachta Indica) and silk fibroin associated hydrogel: Boon for wound healing treatment regimen

Silk Fibroin-Based Anastrozole Nanoparticle Loaded in Situ Injectable: Development and Characterization

Silk Fibroin-Modified Liposome/Gene Editing System Knocks out the PLK1 Gene to Suppress the Growth of Lung Cancer Cells

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