Silk fibroin/amniotic membrane 3D bi-layered artificial skin

Gholipourmalekabadi, Mazaher; Samadikuchaksaraei, Alí; Seifalian, Alexander M.; Urbanska, Aleksandra M.; Ghanbarian, Hossein; Hardy, John G.; Omrani, Mir Davood; Mozafari, Masoud; Reis, Rui L.; Kundu, Subhas C.

doi:10.1088/1748-605x/aa999b

Cited by 103 publications

(120 citation statements)

References 59 publications

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“…Gholipourmalekabadi et al developed a 3D bi-layer scaffold of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) [102]. Adipose tissue-derived mesenchymal stem cells (AT-MSCs), cultured for seven days on the AM-ESF scaffold, demonstrated the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), which suggests potential suitability for AM/ESF scaffold with autologous AT-MSCs [102].…”

Section: Tissue Engineeringmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

Ribeiro

Ferreira

et al. 2020

IJMS

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Burn wounds are highly debilitating injuries, with significant morbidity and mortality rates worldwide. In association with the damage of the skin integrity, the risk of infection is increased, posing an obstacle to healing and potentially leading to sepsis. Another limitation against healing is associated with antibiotic resistance mainly due to the use of systemic antibiotics for the treatment of localized infections. Nanotechnology has been successful in finding strategies to incorporate antibiotics in nanoparticles for the treatment of local wounds, thereby avoiding the systemic exposure to the drug. This review focuses on the most recent advances on the use of nanoparticles in wound dressing formulations and in tissue engineering for the treatment of burn wound infections.

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Section: Tissue Engineeringmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

Ribeiro

Ferreira

et al. 2020

IJMS

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“…The AM/ESF bi-layered 3D artificial skin was prepared and fully characterized in vitro in our recent investigation 12 . Briefly, the AM samples were collected and denuded by a simple and cost-effective method described previously 13 .…”

Section: Preparation Of Decellularized Human Amniotic Membrane/electrmentioning

confidence: 99%

3D Protein-Based Bilayer Artificial Skin for the Guided Scarless Healing of Third-Degree Burn Wounds in Vivo

Gholipourmalekabadi¹,

Seifalian

Urbanska

et al. 2018

Biomacromolecules

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Severe burn injuries can lead to delays in healing and devastating scar formation. Attempts have been made to develop a suitable skin substitute for the scarless healing of such skin wounds. Currently, there is no effective strategy for completely scarless healing after the thermal injuries. In our recent work, we fabricated and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterized both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds. In this report, we evaluate the appropriate utility of this fabricated bilayered artificial skin in vivo with particular emphasis on healing and scar formation due to the biochemical and biomechanical complexity of the skin. For this work, AM and AM/ESF membranes alone or seeded with adipose-tissue-derived mesenchymal stem cells (AT-MSCs) are implanted on full-thickness burn wounds in mice. The healing efficacy and scar formation are evaluated at 7, 14, and 28 days post-implantation in vivo. Our data reveal that ESF accelerates the wound-healing process through the early recruitment of inflammatory cells such as macrophages into the defective site as well as the up-regulation of angiogenic factors from the AT-MSCs and the facilitation of the remodeling phase. In vivo application of the prepared AM/ESF membrane seeded with the AT-MSCs reduces significantly the post-burn scars. The in vivo data suggest that the potential applications of the AM/ESF bilayered artificial skin may be considered a clinical translational product with stem cells to guide the scarless healing of severe burn injuries.

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“…An interesting combination is silk fibroin with decellularized human amniotic membrane, which was used for developing a three-dimensional bi-layered scaffold for burn treatment. Adipose tissue-derived mesenchymal stem cells seeded on this scaffold increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor, and basic fibroblast growth factor [83]. Also the transplantation of bone marrow-derived mesenchymal stem cells and epidermal stem cells into wounds using nanofibrous silk fibroin scaffolds supported re-epithelization, collagen synthesis, as well as the skin appendages regeneration [84].…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Silk fibroin/amniotic membrane 3D bi-layered artificial skin

Cited by 103 publications

References 59 publications

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

3D Protein-Based Bilayer Artificial Skin for the Guided Scarless Healing of Third-Degree Burn Wounds in Vivo

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

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