Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet

Lee, Sun Hee; Park, Dongsun; Yang, Goeun; Bae, Dae-Kwon; Yang, Yaewon; Kim, Tae Kyun; Kim, Dajeong; Kyung, Jangbeen; Yeon, Sungho; Koo, Kyo Chul; Lee, Jun Young; Hwang, Seock-Yeon; Joo, Seong Soo; Kim, Yun-Bae

doi:10.1007/s00394-011-0280-6

Cited by 44 publications

(47 citation statements)

References 35 publications

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“…In addition, that of the SP group significantly decreased compared to that of CON group (* p,0.05). Recent studies have reported that silk peptide ingestion is effective in weight loss for white mice (5,16,18). In the present study, a significant weight loss was also exhibited in the group with 2 wk of exercise and silk peptide intake (800 mg/ kg) compared to that of the control group.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, SP promotes fat oxidation and increases energy expenditure (15,16). Additionally, in several rat and mouse model studies, food intake and body weight were decreased in rodents fed ad libitum (5,14,16).However, measurements in those studies have been conducted for analysis of blood profiles and hormones in mice. Accordingly, we investigated the effects of SP administration using an open circuit calorimetry system on resting energy expenditure and substrate utilization in resting mice for 24 h.…”

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“…Silk peptides (SP) have been ingested for a long time in Asian countries (5). In recent years, additional applications have been developed for silk, mainly in the field of biotechnology and biomedicine.…”

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Silk Peptide Intake Increases Fat Oxidation at Rest in Exercised Mice

Kim

Hwang

Yun

et al. 2013

J Nutr Sci Vitaminol

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250Obesity is associated with lifestyle habits such as inactivity, bad eating practices, stress and excessive net intake of calories (1). Among these, energy imbalance and inactivity are becoming serious problems. Total energy cost can be divided into three parts, including resting metabolic rate (RMR), diet-induced thermogenesis (DIT), and the amount of energy used for physical activity energy expenditure (AEE) (2). The greatest component of daily energy expenditure is RMR, constituting about 70% of the total energy expenditure (3, 4).Silk peptides (SP) have been ingested for a long time in Asian countries (5). In recent years, additional applications have been developed for silk, mainly in the field of biotechnology and biomedicine. The versatility of these new implementations arises from the singularity of the molecular structure of silk (6). Proteins such as fibroin and sericin are the main constituents of silk, with fibroin constituting 70 to 80% and sericin 20 to 30% of the total cocoon weight (6, 7).Many studies regarding the health benefits of SP intake have been reported (8-11). SP not only regulate blood glucose level and hyperlipidemia and but also decrease blood triglycerides and LDL cholesterol (12,13). In addition, a high dose (800 mg/kg) of SP was shown to exert stamina-enhancing activity in forced swimming mice by preventing tissue injuries and increasing anti-fatigue (14). Moreover, it is well known that increasing the concentration of leptin leads to an exothermic reaction and an increase in the amount of activity. Therefore, SP promotes fat oxidation and increases energy expenditure (15,16). Additionally, in several rat and mouse model studies, food intake and body weight were decreased in rodents fed ad libitum (5,14,16).However, measurements in those studies have been conducted for analysis of blood profiles and hormones in mice. Accordingly, we investigated the effects of SP administration using an open circuit calorimetry system on resting energy expenditure and substrate utilization in resting mice for 24 h. Materials and MethodsAnimals. Seven-week-old male ICR-mouse (n518) were used. Mice were purchased from Orient Bio Inc. (Seongnam, Korea). All mice were housed in standard plastic cages under controlled conditions of humidity (50%) and temperature (2361˚C) with alternating 12 h cycles of light and dark. Mice were adapted to the laboratory housing conditions for 3 d. They were given free access to water and a non-purified commercial diet (5L79, Orient Bio Inc.) containing (g/kg diet): crude protein, 180; crude fat, 52; crude fiber, 52; minerals, 57; and carbohydrate, 368. The protein, fat, and carbohydrate ratio (%) based on calories was 21 : 14 : 65, and (Received January 11, 2013) Summary Silk peptides (SP) have been reported to decrease body weight and accumulate fat. We investigated the effects of SP administration by using an open circuit calorimetry system on resting energy expenditure and substrate utilization in resting mice for the duration of 24 h. Seven-week-old male ICR-...

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Silk Peptide Intake Increases Fat Oxidation at Rest in Exercised Mice

Kim

Hwang

Yun

et al. 2013

J Nutr Sci Vitaminol

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“…In 2012, Lee et al 8 . reported that treatment with 1 mg/mL SP + 0.2 nM insulin increases glucose uptake (124 ± 2.5%) via upregulation of glucose transporter type 4 (GLUT4) and decreases fat accumulation via upregulation of leptin in 3T3-L1 preadipocytes.…”

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confidence: 99%

Effects of different doses of silk peptide on energy metabolism during exercise in mice

Suh¹,

Kim²,

Park³

et al. 2016

Journal of Exercise Nutrition &Amp; Biochemistry

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Silkworm pupae as source of high‐value edible proteins and of bioactive peptides

Altomare

Baron

Aldini

et al. 2020

Food Science & Nutrition

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To characterize the high‐value protein content and to discover new bioactive peptides, present in edible organisms, as silkworm pupae, semiquantitative analytical approach has been applied. The combination of appropriate protein extraction methods, semiquantitative high‐resolution mass spectrometry analyses of peptides, in silico bioactivity and gene ontology analyses, allowed protein profiling of silkworm pupae (778 gene products) and the characterization of bioactive peptides. The semiquantitative analysis, based on the measurement of the emPAI, revealed the presence of high‐abundance class of proteins, such as larval storage protein (LSP) class. This class of proteins, beside its nutrient reservoir activity, is of great pharmaceutical interest for their efficacy in cardiovascular diseases. Potential allergens were also characterized and quantified, such as arginine kinase, thiol peroxiredoxin, and Bom m 9. This powerful bioanalytical approach proved the potential industrial applications of Bombyx mori pupae, as source of high‐value proteins in a green and “circular” economy perspective.

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Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet

Abstract: SP and SPP inhibit the differentiation of preadipocytes and adipogenesis by modulating signal transduction pathways and improve HFD-induced obesity by reducing lipid accumulation and the size of adipocytes.

Cited by 44 publications

References 35 publications

Silk Peptide Intake Increases Fat Oxidation at Rest in Exercised Mice

Silk Peptide Intake Increases Fat Oxidation at Rest in Exercised Mice

Effects of different doses of silk peptide on energy metabolism during exercise in mice

Silkworm pupae as source of high‐value edible proteins and of bioactive peptides

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