Silicone based polyurethane materials: a promising biocompatible elastomeric formulation for cardiovascular applications

Abstract

The biocompatibility of a new material for cardiovascular applications constituted by a poly(ether)urethane (PEtU) and a silicone [polydimethylsiloxane (PDMS)] was evaluated. The achieved material shows properties similar to both polyurethanes and silicones. The material was transformed into porous membranes by a spray-deposition technique. Since any material preparation and manufacturing procedure may introduce some toxicity, in vitro cytotoxicity screening tests were carried out. Human umbilical vein endothelial cells (HUVECs) and a mouse fibroblasts cell line (L929) were cultivated with extracts obtained from materials containing 10, 40 and 100% (w/w) of PDMS. The commercially available Estane 5714-F1 and Cardiothane 51 were used as controls. Extracts were incubated up to 72 hours with HUVECs and L929 cells. The cytotoxic effect was evaluated by light microscopy, cell viability (MTT reduction and neutral red uptake) and proliferation (5-bromo-2'-deoxyuridine incorporation) tests. In vivo studies were carried out using materials containing the same PDMS percentages as for in vitro experiments. The same commercial controls were used. Results obtained with cell culture studies agreed with those obtained in the in vivo experiments and showed that the material preparation and manufacturing procedure do not introduce any toxicity in the products at each PDMS concentration investigated.

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

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Tissue response to poly(ether)urethane-polydimethylsiloxane-fibrin composite scaffolds for controlled delivery of pro-angiogenic growth factors

“…Our group has already shown the good biocompatibility and mechanical properties of the synthetic layer made of a PEtUePDMS semi-IPN material [29,33].…”

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Polyurethane for biomedical applications: A review of recent developments

“…Silicon was introduced into the polyurethane, in order to address the biodegradation problems occurring in long-term implantation. Briganti et al (2006) synthesized silicon based polyurethane materials and studied the biocompatibility of the resulted polyurethane. It was concluded that the incorporation of silicone in the investigated range did not introduce any toxicity into the materials; however, further studies related to biostability and mechanical properties of the new material are necessary.…”