Silicon substrate as a novel cell culture device for myoblast cells

Abstract

BackgroundTissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy.ResultsIt was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate.ConclusionsThis study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.

Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility

“…A monocrystalline silicon wafer has been chosen as an experimental substrate because silicon crystals are sensitive to mechanical and thermal excitation. Silicon is increasingly being considered for bio-applications 20 21 22 . Aside from biological in favor of using silicon as a bio-template, slight changes in crystal orientation of a silicon substrate leads to the build-up of a residual stress component.…”

Photovoltaic surfaces enable clonal myoblastic cell release using visible light as external stimulation

“…Silicon based PV devices in the dimension of 2 cm × 4 cm were obtained from RadioShack ® , Model 276‐124. A non‐toxic biocompatible glue wall provided surrounding to the PV devices to avoid spilling cell culturing liquid medium . PV devices were sterilized by exposing them to ultra violet light in a homebuilt chamber for 2.5 min to remove surface contaminations .…”

“…We hypothesize that by exposing electrically excitable cells grown on PV devices to light, enough electromotive forces are generated to release some cells from the culturing surface in a novel non‐invasive way. To proof this concept we evaluated the effect of external light on a model cell line, the mouse myoblast cell line C2C12, grown on PV devices . Disadvantages of this method include the limited light penetration through tissues.…”

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

“…It is a high‐reliable and highly biocompatible material, which demonstrated to allow SH‐SY5Y cell adhesion and growth . Silicon is widely used in biomedical applications due to the possibility to integrate electronics and biological systems . For instance, it is used in electrode‐neuron implants and in functional electrode stimulation .…”