Silicon Nanowire-Based Molecular Beacons for High-Sensitivity and Sequence-Specific DNA Multiplexed Analysis

Su, Shao; Wei, Xinpan; Zhong, Yiling; Guo, Yuanyuan; Su, Yuanyuan; Huang, Qing; Lee, Shuit‐Tong; Fan, Chunhai; He, Yao

doi:10.1021/nn2050449

Cited by 101 publications

(89 citation statements)

References 56 publications

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“…The limits of detection (LOD) of the targets calculated according to the 3δ were 75 pM for T1, and 62 pM for T2. Which is comparable or more sensitive than most of the methods previously reported for multiplexed analysis of DNA by AuNPs, GO, carbon nanotube and silicon nanowire [10,11,13,15] (See table S1, ESM). These results indicated that g-C 3 N 4 based multicolor nanoprobes can be applied to quantify multiple analytes with high sensitivity in homogenous solution.…”

Section: Multiplexed Dna Detectionmentioning

confidence: 72%

“…Fan et al reported using gold nanoparticles (AuNPs) to construct multicolor nanoscale molecular beacon for sequence specific nucleic acid analysis [10]. Carbon nanomaterial such as graphene oxide (GO) and carbon nanotube have been also exploited to develop multicolor fluorescent nanoprobes for multiplexed analysis of DNA [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Graphitic carbon nitride nanosheet-based multicolour fluorescent nanoprobe for multiplexed analysis of DNA

Zhong

Huang

et al. 2014

Microchim Acta

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We demonstrate that nanosheets composed of graphitic carbon nitride (g-C 3 N 4 ) can serve as a low-cost and efficient fluorescent nanoprobe for the multiplexed detection of DNA in solution. The strategy is based on the finding that g-C 3 N 4 is capable of binding dye-labeled single-stranded DNA (ssDNA) which results in quenching of the fluorescence of the dye. If target DNA hybridizes with dye-labeled ssDNA, the interaction between dye-labeled ssDNA and g-C 3 N 4 is weakened, and this results in desorption of the dsDNA from the surface of the g-C 3 N 4 and in recovery of fluorescence. The large surface area of g-C 3 N 4 nanosheets allows for simultaneous quenching of multicolor DNA probes labeled with different dyes, leading to the development of multiplexed DNA sensors for the detection of multiple DNA targets in a single solution. By using one 15-mer DNA fragment and one 18-mer DNA fragment as proof-of-principle analytes, the method displayed good analytical performance. The limits of detection are 75 and 62 pM, respectively. The method is simple and sensitive, and was used to detect DNA in serum samples. We perceive that this method represents a new approach towards multiplexed assays for applications in DNA monitoring, clinical diagnosis, and in the detection of genetic disorders.

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Section: Multiplexed Dna Detectionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Graphitic carbon nitride nanosheet-based multicolour fluorescent nanoprobe for multiplexed analysis of DNA

Zhong

Huang

et al. 2014

Microchim Acta

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“…In addition to SiNWs-based FET, scientists have made tremendous effort for designing SiNW-based optical biosensors (e.g., fluorescent-and SERS-based biosensors) for detection of tumor-suppressor genes in sensitive, specific and reproducible manners. In 2012, He and coworkers employed silicon nanohybrids made of AuNPs@SiNWs for constructing novel molecular beacons (nanoMBs) with robust salt stability and high fluorescence quenching efficiency (> 90%) [48]. In comparison with free AuNPs which were prone to be aggregated in low-concentration (e.g., 0.01 M) salt solution, the SiNW-based nanoMBs preserved robust stability at a high concentration of salt solution (i.e., 0.1 M).…”

Section: Silicon Nanomaterial-based Biosensors For Cancer Diagnosismentioning

confidence: 99%

Silicon Nanostructures for Cancer Diagnosis and Therapy

Peng

Cao

et al. 2015

Nanomedicine (Lond.)

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The emergence of nanotechnology suggests new and exciting opportunities for early diagnosis and therapy of cancer. During the recent years, silicon-based nanomaterials featuring unique properties have received great attention, showing high promise for myriad biological and biomedical applications. In this review, we will particularly summarize latest representative achievements on the development of silicon nanostructures as a powerful platform for cancer early diagnosis and therapy. First, we introduce the silicon nanomaterial-based biosensors for detecting cancer markers (e.g., proteins, tumor-suppressor genes and telomerase activity, among others) with high sensitivity and selectivity under molecular level. Then, we summarize in vitro and in vivo applications of silicon nanostructures as efficient nanoagents for cancer therapy. Finally, we discuss the future perspective of silicon nanostructures for cancer diagnosis and therapy.

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“…Of particular significance, the resultant SiNWs-based MBs were highly stable in solutions with high salt concentrations (e.g., 0.1 M) and in a wide temperature range (e.g., 10-80°C). Besides, the silicon MBs were suitable for simultaneously assembling various DNA strands due to huge surface of the prepared AuNPs-coated SiNW, allowing sensitive and multiplexed DNA detection at *pM levels [113].…”

Section: Fluorescence-based Biosensorsmentioning

confidence: 99%

Silicon-Based Platform for Biosensing Applications

He¹,

Su²

2014

SpringerBriefs in Molecular Science

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Development of high-performance biosensors vastly facilitates the analysis and detection of various biological species, including nucleic acids, protein, cell, etc. Functional nanomaterials (e.g., silver/gold nanoparticles, carbon nanotubes, graphene, silicon nanowires, etc) serve as new platform for design of nano-biosensors featuring high sensitivity and specificity. Taking advantage of the attractive merits of silicon nanowires (SiNWs) (e.g., unique electronic/optical properties, huge surface-to-volume rations, surface tailorability, fast response and good reproducibility, and compatibility with conventional silicon technology), SiNWs have been widely employed for constructing various kinds of electrochemical and optical biosensors, enabling ultrasensitive, specific, and reproducible detection of DNA and protein. We introduce a number of typical SiNWs-based biosensors (e.g., field-effect transistor (FET), amperometric-, surface-enhanced Raman scattering (SERS), and fluorescence-based biosensors) in this chapter, aiming to summarize the representative progresses of this research field in recent years. These kinds of high-quality silicon-based sensors show potentially great promise for myriad practical applications, such as medical diagnosis, food safety, drug security, environment monitoring, as well as anti-bioterrorism and so forth.Keywords Silicon nanowires Á Biosensor Á Field effect transistor Á Surfaceenhanced Raman scattering (SERS) Á DNA and protein detection Á Sensitivity and specificity Biological/chemical analysis and detection are of essential importance for disease diagnosis, drug discovery, food safety, environmental protection, and anti-bioterrorism, etc. While a large number of bioassay kits have been available on the market, there are ever-growing efforts to develop new detection methods with ultrahigh sensitivity and specificity, to meet the increasing demands of biosensing applications. Parallel to research efforts devoted to such high-end requirements, much recent interest has been directed toward the development of low-cost and portable biosensors, which possess comparable high sensitivity to conventional instruments, but with greatly reduced cost/mass/power requirements. Nanostructures (e.g., nanoparticles [1][2][3][4][5][6][7][8][9], nanotubes [10][11][12][13][14], and nanowires [15][16][17][18], etc) Y.

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Silicon Nanowire-Based Molecular Beacons for High-Sensitivity and Sequence-Specific DNA Multiplexed Analysis

Cited by 101 publications

References 56 publications

Graphitic carbon nitride nanosheet-based multicolour fluorescent nanoprobe for multiplexed analysis of DNA

Graphitic carbon nitride nanosheet-based multicolour fluorescent nanoprobe for multiplexed analysis of DNA

Silicon Nanostructures for Cancer Diagnosis and Therapy

Silicon-Based Platform for Biosensing Applications

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