Silicon microgrooves for contact guidance of human aortic endothelial cells

Abstract

Background: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour. Results: The influence of groove-shaped substrates on cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect. Conclusion: The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with microscale features to mimic the endothelium in lineal vessels.

Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures

“…The efficiency of 3-aminopropyltrietoxysilane (APTES) + GTA-modified porous surfaces in immobilizing extracellular matrix proteins, such as collagen (Col) or fibronectin (Fn) and, the biocompatibility of these modified surfaces for the adhesion and proliferation of human aortic endothelial cells (HAEC) have been studied in this work using NAA and PSi as substrates. Previously, we have reported the development of Col-coated silicon microstructures to study the effect of the topography on the behaviour of HAEC [ 15 , 16 , 42 ]. HAEC cell line is one of the most commonly used models in the study of the endothelial dysfunction and its capacity to adhere to the substrate and to produce cell adhesion molecules make them a good tool for screening emerging cardiovascular therapies [ 43 ].…”

Micropatterned Fibrous Scaffold Produced by Using Template-Assisted Electrospinning Technique for Wound Healing Application

“…By increasing the incubation time, the HDF cells favorably moved, migrated and proliferated along the triangular prism of the structural scaffold. The HDF cells on the structural scaffold exhibited a similar elongation and orientation behavior in comparison with cell morphologies on aligned nanofibers or anisotropic topography [ 3 , 7 , 17 , 29 , 30 , 31 , 32 , 33 ].…”

An Engraved Surface Induces Weak Adherence and High Proliferation of Nonadherent Cells and Microorganisms During Culture

“…A broad range of techniques and materials have been employed to fabricate well-defined topographical and chemical cues to assess cell micropatterning. Some of these fabrication techniques are based on photolithography and reactive ion etching that may be followed by anisotropic etching [19]. In recent years, microfluidics encompassing sophisticated micropatterning designs has led to the development of powerful tools for single cell analysis.…”