Silica-supported dual-dye nanoprobes for ratiometric hypoxia sensing

Wang, Chang; Chen, Yuzhe; Yang, Qing‐Zheng; Tung, Chen‐Ho; Wu, Dayong; Wu, Li‐Zhu

doi:10.1039/d0qm00653j

Cited by 6 publications

(6 citation statements)

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“…The Stern–Volmer constants ( K SV ) were obtained as 449.3 bar −1 , indicating its very high sensitivity to oxygen. [ 23–26,44–51 ] This value is significantly improved in comparison to our previously reported supramolecular polymer‐based oxygen nanoprobe ( K SV = 189.6 bar −1 ). [ 26 ] The limit of detection (LOD) was as low as 0.223 Pa when 0.1% change of the ratiometric emission intensity ratio of BrNpA‐SiNPs in fully deoxygenated and partially oxygenated solutions occurs.…”

Section: Resultsmentioning

confidence: 64%

“…Particularly, herein using an internal fluorescence standard rather than an external one in ratiometric imaging will result in better sample homogeneity and minimized effect from different dye stabilities. [ 22–25 ]…”

Section: Resultsmentioning

confidence: 99%

“…In search of purely organic materials with bright phosphorescence in aqueous solution, we initiated to incorporate organic phosphors with silica nanoparticles by covalent bonds. [ 15–23 ] Silica nanoparticles have the ability to inhibit non‐radiative dissipation from singlet excited states. However, it remains largely unexplored if the covalent incorporation of pure organic chromophores with rigid polysiloxane framework can promote bright phosphorescence in water.…”

Section: Introductionmentioning

confidence: 99%

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Ratiometric hypoxia detection by bright organic room temperature phosphorescence of uniformed silica nanoparticles in water

Guo

Chen

et al. 2022

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Organic room temperature phosphorescence (RTP) in water has attracted much attention recently for its potential biological applications. However, it remains a formidable challenge to achieve efficient RTP from pure organic compounds in aqueous phase due to the dramatic deactivation of triplet excited states in water and the poor water dispersibility of large organic particles/crystals. Represented herein is covalent incorporation of a pure organic monochromophore in silica nanoparticles (SiNPs) featuring fluorescence and bright phosphorescence in aqueous solution. The covalent bonding of organic phosphors in polysiloxane framework was found to show excellent water dispersibility, at the same time suppress the non‐radiative deactivation of triplet excited states especially from water, thus leading to high phosphorescence quantum yields (up to 22%) and long lifetimes (up to 3.5 ms) in aqueous phase. More strikingly, oxygen‐insensitive fluorescence as internal reference and oxygen‐dependent phosphorescence as oxygen indicator from the organic chromophore in the porous SiNPs realized ratiometric hypoxia detection with ultrasensitivity (KSV = 449.3 bar−1).

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Section: Resultsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ratiometric hypoxia detection by bright organic room temperature phosphorescence of uniformed silica nanoparticles in water

Guo

Chen

et al. 2022

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“…Q factor was calculated to be 98.4% (or I 0 / I air = 60.7) for 2TA2TA:PMMA film, which is at the forefront of oxygen sensing performance. [ 4,15,28 ] Furthermore, 2TA2TA:PMMA film shows good reversibility and reproducibility of emission spectra between vacuum and air environment (Figure S19, Supporting Information). In addition, UV light irradiation of 2TA2TA for 32 h did not bring about any change in 1 H NMR spectrum, indicative of the excellent photochemical stability of TA analogues (Figure S20, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Dual‐Emission of Fluorescence and Room‐Temperature Phosphorescence for Ratiometric and Colorimetric Oxygen Sensing and Detection Based on Dispersion of Pure Organic Thianthrene Dimer in Polymer Host

Liu

Pan

Yang

et al. 2022

Advanced Optical Materials

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To improve room‐temperature phosphorescence (RTP) property, thianthrene (TA) is chemically modified into three intramolecular dimers (1TA1TA, 1TA2TA, and 2TA2TA) at different active sites. They all show dual‐emission of fluorescence and RTP after deoxygenation when they are dispersed in polymer matrix to form films. As for the RTP emission among their films, 2TA2TA shows the largest red shift peaking at 540 nm, the longest lifetime of 36.5 ms, and the highest RTP efficiency of 40.7%. These RTP characteristics enable 2TA2TA to act as a self‐referenced ratiometric and colorimetric probe for oxygen sensing and detection at low concentration. As a result, 2TA2TA exhibits the highest quenching efficiency of 98.4% and highest sensitivity (KSV = 13.32 KPa−1) for quantitative oxygen detection. Besides, these oxygen‐sensitive materials can also be used to study aerodynamics through a direct and visual observation of emission intensity/color distribution. Overall, the new‐generation optical probe will be developed in oxygen sensing and detection with high sensitivity, good reversibility, and reproducibility by using the pure organic materials with single‐molecule dual‐emission of fluorescence and RTP through the dispersion of the materials in polymeric host.

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“…With our long-term interests in quantitative oxygen detection, [36][37][38][39] we report herein a facile molecular engineering strategy by bridging carbazoles with an ethoxysilane as a spacer, namely DCzC2OSi (Figure 1). Importantly, the DCzC2OSi crystals displayed both ultralong lifetime under vacuum (741.5 ms) and oxygen sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Ultralong Room-Temperature Phosphorescence of Silicon-Based Pure Organic Crystal for Oxygen Sensing

et al. 2022

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Quantitative oxygen detection is of great importance in biological fields, complex environments and chemical process engineering. Due to the high sensitivity and fast response of long-lived phosphorescence to oxygen, pure organic room temperature phosphorescence (RTP) for oxygen detection has recently attracted much interests. However, to simultaneously achieve ultralong phosphorescence at room temperature and quantitative oxygen detection from pure organic crystals is difficult because tight packing for restricting nonradiative decays is not apt to allow oxygen diffusion for sensing. Reported herein is such an exceptional example, i.e. a crystal of simple carbazole molecule bridging with an ethoxysilane (DCzC2OSi), is capable of oxygen sensing with remarkable sensitivity. Photophysical studies and single crystal structure analysis reveal that DCzC2OSi crystals display ultralong RTP and suitable oxygen diffusion channels from the butterfly-like tetrahedron geometry. Further comparisons with the crystals of CzC2OH and DCzSi verify the important roles of silicon and ethoxy groups of DCzC2OSi for both enhanced phosphorescence lifetime and oxygen sensitivity.When the crystals of DCzC2OSi were doped into polymer, the lifetime-based oxygen sensor exhibits high K SV (5.308 kPa -1 ) with full reversibility, which would be attractive to the future development of practical oxygen sensors from pure organic crystals.

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Silica-supported dual-dye nanoprobes for ratiometric hypoxia sensing

Abstract: Oxygen (O2) levels provide an important parameter in the early-stage diagnosis of cancers and other diseases. Ratiometric optical O2 sensing has attracted much interest due to its better calibration of...

Cited by 6 publications

References 50 publications

Ratiometric hypoxia detection by bright organic room temperature phosphorescence of uniformed silica nanoparticles in water

Ratiometric hypoxia detection by bright organic room temperature phosphorescence of uniformed silica nanoparticles in water

Dual‐Emission of Fluorescence and Room‐Temperature Phosphorescence for Ratiometric and Colorimetric Oxygen Sensing and Detection Based on Dispersion of Pure Organic Thianthrene Dimer in Polymer Host

Ultralong Room-Temperature Phosphorescence of Silicon-Based Pure Organic Crystal for Oxygen Sensing

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