Silica precipitation with synthetic silaffin peptides

Wieneke, Ralph; Bernecker, Anja; Riedel, R.; Sumper, Manfred; Steinem, Claudia; Geyer, Armin

doi:10.1039/c1ob05406f

Cited by 56 publications

(64 citation statements)

References 19 publications

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“…These residues enable silaffins and peptides derived from them to induce silica precipitation in vitro [16][17][18][19][20] . One such peptide is R5, derived from the silaffin protein Sil1 of Cylindrotheca fusiformis.…”

Section: Introductionmentioning

confidence: 99%

Studying the Conformation of a Silaffin-Derived Pentalysine Peptide Embedded in Bioinspired Silica using Solution and Dynamic Nuclear Polarization Magic-Angle Spinning NMR

et al. 2016

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Smart materials are created in nature at interfaces between biomolecules and solid materials. The ability to probe the structure of functional peptides that engineer biogenic materials at this heterogeneous setting can be facilitated tremendously by use of DNP-enhanced solid-state NMR spectroscopy. This sensitive NMR technique allows simple and quick measurements, often without the need for isotope enrichment. Here, it is used to characterize a pentalysine peptide, derived from a diatom's silaffin protein. The peptide accelerates the formation of bioinspired silica and gets embedded inside the material as it is formed. Two-dimensional DNP MAS NMR of the silica-bound peptide and solution NMR of the free peptide are used to derive its secondary structure in the two states and to pinpoint some subtle conformational changes that the peptide undergoes in order to adapt to the silica environment. In addition, interactions between abundant lysine residues and silica surface are identified, and proximity of other side chains to silica and to neighboring peptide molecules is discussed.

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Section: Introductionmentioning

confidence: 99%

Studying the Conformation of a Silaffin-Derived Pentalysine Peptide Embedded in Bioinspired Silica using Solution and Dynamic Nuclear Polarization Magic-Angle Spinning NMR

et al. 2016

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“…Silaffins are associated with siliceous frustules of diatoms and the hypothesis of biogenic silica growth around matrix of silaffins was formulated. 140,141,143 Unfortunately, silaffins can be isolated from diatom frustules in microgram quantities only which is not sufficient for study of their properties including their inuence on silicic acid condensation. Hence, synthetic organic polymers can serve as a good model of silaffins.…”

Section: Polymer : Si Ratiomentioning

confidence: 99%

“…24,61,62,[65][66][67][68][69][70][71][75][76][77]98,99,[106][107][108]111,112,114,117,121,124,134,135,137,139,143,149,153,155,157 These variants exist in the case of relatively low silicon content in the system, when all PSA nanoparticles can nd chains of organic polymer. Further increase in the silicic acid content results in formation of insoluble non-uniform products 64,83 because of cross-linking by means of silicic acid which was condensed independent of the polymer control.…”

Section: Polymer : Si Ratiomentioning

confidence: 99%

Silicic acid condensation under the influence of water-soluble polymers: from biology to new materials

et al. 2017

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Silicon is among the most abundant elements on the Earth. It occurs in many minerals and plays an important role in several biochemical processes. Some living organisms use silicon dioxide as a substrate for building elements of their bodies. Unicellular diatom algae build frustules from silicon dioxide. The skeleton of siliceous sponges is a silicaprotein composite. Similarly, rice hulls which protect seeds, contain silica as an important component. The living organisms assimilate silicon from the environment in the form of silicic acid. However, the biochemical mechanisms involved in the transformation of silicic acid to solid siliceous materials are still poorly understood.Evidently, condensation of silicic acid in the living organisms proceeds under control of biopolymers and it is important to know how various types of polymers influence the condensation. Bio-inspired chemistry involving the interaction between polymeric silicic acid and functional polymers results in interesting composite materials, including nanoparticles and bulk materials. This review contains a brief description of the mechanism of silicic acid condensation in aqueous medium and also includes a discussion on various precursors of silicic acid. The main focus of the review is on the influence of polymers bearing nitrogen and oxygen-containing functional groups on silicic acid condensation starting from monomer to three-dimensional polymer. Influence of molecular weight of the organic polymer on the condensation and structure of the resulting product is also elaborated. The biological importance of the obtained data and strategies for novel applications of the synthesized composite materials are described in the concluding section of the review. The biomimetic condensation processes open up new vistas for development of novel materials and applications in the biomedical and process industries.

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“…However, it is known that silaffins 1,2 and long-chain polyamines 3 are involved in valve synthesis within a specialized unit in the parent diatom known as the silica deposition vesicle (SDV) 4 . Unravelling these mechanisms has enabled the generation of synthetic silica structures in vitro under mild conditions using synthetic silaffins and long-chain polyamines [5][6][7] . The alkoxysilane tetramethoxysilane (TMOS) has been shown to form SiO 2 in the presence of silaffins in vitro 8 following a typical sol-gel process whereby the alkoxide undergoes hydrolysis and the resulting sol gradually evolves towards the formation of a continuous colloidal network 9 .…”

mentioning

confidence: 99%

Functionalization of the living diatom Thalassiosira weissflogii with thiol moieties

et al. 2013

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Biomineralization processes identified within diatoms have inspired the design of synthetic silica structures in vitro using alkoxysilane precursors. Here we explore the use of the machinery within the living diatom to fabricate organo-silica constructs using a combination of alkoxysilane and organoalkoxysilane precursors. We report on the incorporation of thiol moieties into the diatom during frustule synthesis. Formation of valves within the parent diatom is monitored using fluorescence microscopy, and the modification of the chemical composition of the diatom is confirmed using energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy and 29 Si-nuclear magnetic resonance spectroscopy. Chemical modification is achieved without loss of the nano-scale architectural features of the frustule. Extension of this work may allow the chemistry of the diatom to be tailored during synthesis.

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Silica precipitation with synthetic silaffin peptides

Cited by 56 publications

References 19 publications

Studying the Conformation of a Silaffin-Derived Pentalysine Peptide Embedded in Bioinspired Silica using Solution and Dynamic Nuclear Polarization Magic-Angle Spinning NMR

Studying the Conformation of a Silaffin-Derived Pentalysine Peptide Embedded in Bioinspired Silica using Solution and Dynamic Nuclear Polarization Magic-Angle Spinning NMR

Silicic acid condensation under the influence of water-soluble polymers: from biology to new materials

Functionalization of the living diatom Thalassiosira weissflogii with thiol moieties

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