Silica-polydopamine hybrids as light-induced oxidase mimics for colorimetric detection of pyrophosphate

Lin, Zhen; Luo, Shuang; Xu, Dafen; Liu, Shijun; Wu, Namei; Yao, Wensong; Zhang, Xiaomin; Zheng, Linlin; Lin, Xinhua

doi:10.1039/c9an01813a

Cited by 19 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This work polydopamine nanozyme possess features of unique multi‐antioxidative, anti‐inflammatory and biocompatible. Furthermore, other non‐metallic polymer nanozymes, such as, the silica‐polydopamine hybrids, [ 167 ] polystyrene, [ 168 ] and the GO‐Se nanocomposites [ 169 ] were all utilized as nanozymes.…”

Section: Materials For Nanozyme Fabricationmentioning

confidence: 99%

Recent Advances in Nanozymes: From Matters to Bioapplications

Liang

et al. 2021

Adv Funct Materials

167

115

View full text Add to dashboard Cite

The manufacture of bionic materials to simulate the natural counterparts has attracted extensive attention. As one of the subcategories of biomimetic materials, the development of artificial enzyme is intensive pursuing. As a kind of artificial enzyme, nanozymes are dedicated to solve the limitations of natural enzymes. In recent years, attributed to the explosive development of nanotechnology, biotechnology, catalysis science, computational design and theory calculation, research on nanozymes has made great progress. To highlight these achievements and help researchers to understand the current investigation status of nanozyme, the state‐of‐the‐art development in nanozymes from fabrication materials to bioapplications are summarized. First different raw materials are summarized, including metal‐based, metal‐free, metal‐organic frameworks‐based, and some other novel matters, which are applied to fabricate nanozymes. The different types of enzymes‐like catalytic activities of nanozymes are briefly discussed. Subsequently, the wide applications of nanozymes such as anti‐oxidation, curing diseases, anti‐bacteria, biosensing, and bioimaging are discussed. Finally, the current challenges faced by nanozymes are outlined and the future directions for advancing nanozyme research are outlooked. The authors hope this review can inspire research in the fields of nanotechnology, chemistry, biology, materials science, and theoretical computing, and can contribute to the development of nanozymes.

show abstract

Section: Materials For Nanozyme Fabricationmentioning

confidence: 99%

Recent Advances in Nanozymes: From Matters to Bioapplications

Liang

et al. 2021

Adv Funct Materials

167

115

View full text Add to dashboard Cite

show abstract

“…Nanomaterials with enzyme‐like properties (nanozymes) have provoked researchers′ curiosity worldwide because of their ease of preparation, scale‐up, extended storage length, excellent stability, broad pH/temperature tolerance compared to bio‐enzymes, and low‐cost availability [11–13] . This nanostructured material can detect a wide range of metabolites by mimicking enzyme functions such as oxidase, peroxidase, catalase, and superoxide dismutase without having any structural resemblance to natural enzymes [14–21] . Peroxidase‐like biomimics are the most well‐studied inorganic nanozymes for bioanalytes detection and immunoassay applications [22] .…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13] This nanostructured material can detect a wide range of metabolites by mimicking enzyme functions such as oxidase, peroxidase, catalase, and superoxide dismutase without having any structural resemblance to natural enzymes. [14][15][16][17][18][19][20][21] Peroxidase-like biomimics are the most well-studied inorganic nanozymes for bioanalytes detection and immunoassay applications. [22] However, using a strong oxidizing agent, H 2 O 2, in a peroxidase-like catalytic reaction is mandatory.…”

Section: Introductionmentioning

confidence: 99%

Vanadium‐Incorporated Mesoporous Silica as Oxidase Mimic for Colorimetric Dopamine Detection and Anticancer Activity

et al. 2023

View full text Add to dashboard Cite

The intriguing features of artificial enzymes made nanozymes attractive in the field of biosensing and biomedical. Here, vanadium‐incorporated dendritic mesoporous silica (VMSN) as an oxidase mimicking nanozyme was utilized as a sensitive and selective colorimetric biosensor to detect dopamine (DA) and treat cancer cells. The nanozyme was successfully fabricated through the in‐situ incorporation of vanadium ions in the silica framework via a simple sol‐gel method using CTAB and mesitylene. The excellent oxidase‐like activity of VMSN was monitored by the oxidation of TMB. The kinetics, mechanism of the catalytic reaction and the effect of temperature, pH, and concentration on oxidase‐like activity were investigated thoroughly and further, based on the oxidase‐like activity, a fast and sensitive colorimetric sensing of dopamine developed with a LOD of 0.0021 μM and a linear range of 0–50 μM. The proposed colorimetric method was successfully used to determine DA concentration in commercially available dopamine hydrochloride injection.VMSN was further modified with folic acid and utilized against cancer cells in vitro. Neuroblastoma cells (SHSY‐5Y) show ROS generated by VMSN in the presence of oxygen, leading to enhanced cytotoxicity detrimental for SHSY‐5Y cells.

show abstract

“…Until now, a variety of different techniques have been devoted to conducting PPi detection, including fluorescence spectroscopy [ 5 , 6 , 7 ], surface-enhanced Raman-scattering (SERS) assays [ 8 ], colorimetric sensing [ 9 , 10 , 11 ], chemiluminescence [ 12 ], electrochemical analysis [ 13 , 14 ], and dark-field optical microscopy (DFM) [ 3 ]. The principle of most of the above methods is based on the complexation of PPi with metal ions (Al 3+ [ 15 ], Fe 3+ [ 9 , 16 ], Ca 2+ [ 11 ], Zn 2+ [ 17 ], and Cu 2+ [ 5 , 6 , 10 , 12 , 13 , 18 ]), as PPi is a good complexing agent. From the above, fluorescence spectroscopy and colorimetric sensing are relatively common methods for detecting PPi.…”

Section: Introductionmentioning

confidence: 99%

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Shi

Wang

et al. 2021

Sensors

View full text Add to dashboard Cite

Copper (II) ions have been shown to greatly improve the chemical stability and peroxidase-like activity of gold nanoclusters (AuNCs). Since the affinity between Cu2+ and pyrophosphate (PPi) is higher than that between Cu2+ and AuNCs, the catalytic activity of AuNCs-Cu2+ decreases with the introduction of PPi. Based on this principle, a new colorimetric detection method of PPi with high sensitivity and selectivity was developed by using AuNCs-Cu2+ as a probe. Under optimized conditions, the detection limit of PPi was 0.49 nM with a linear range of 0.51 to 30,000 nM. The sensitivity of the method was three orders of magnitude higher than that of a fluorescence method using AuNCs-Cu2+ as the probe. Finally, the AuNCs-Cu2+ system was successfully applied to directly determine the concentration of PPi in human urine samples.

show abstract

Silica-polydopamine hybrids as light-induced oxidase mimics for colorimetric detection of pyrophosphate

Abstract: Fluorescent silica-polydopamine hybrids have the light-induced oxidase-mimicking activity. The activity was quenched by Cu2+ and recovered by pyrophosphate ion (PPi), based on which, a colorimetric PPi detection method was established.

Cited by 19 publications

References 43 publications

Recent Advances in Nanozymes: From Matters to Bioapplications

Recent Advances in Nanozymes: From Matters to Bioapplications

Vanadium‐Incorporated Mesoporous Silica as Oxidase Mimic for Colorimetric Dopamine Detection and Anticancer Activity

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Contact Info

Product

Resources

About