Silica nanoparticles induce spermatocyte cell apoptosis through microRNA-2861 targeting death receptor pathway

Ren, Lihua; Zhang, Jin; Wang, Ji; Wei, Jialin; Liu, Jianhui; Li, Xiangyang; Zhu, Yupeng; Li, Yanbo; Guo, Caixia; Duan, Junchao; Sun, Zhiwei; Zhou, Xianqing

doi:10.1016/j.chemosphere.2019.04.116

Cited by 20 publications

(11 citation statements)

References 47 publications

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“…Previous studies have shown that SiO 2 NPs reduce the activity and quantity of sperm in mice and are associated with apoptosis of spermatogenic cells (Ren et al, 2016(Ren et al, , 2019. In our experiments, SiO 2 NPs exposure significantly reduced the reproductive ability of nematodes, so we speculated that nanoparticles may enhance the apoptosis of germ cells.…”

Section: Sio 2 Nps Enhanced Germ Cell Apoptosissupporting

confidence: 46%

Silica nanoparticles enhance germ cell apoptosis by inducing reactive oxygen species (ROS) formation in <i>Caenorhabditis elegans</i>

et al. 2020

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Silica nanoparticles (SiO 2 NPs) are widely used in daily life and can enter organisms through several pathways, often causing unpredictable toxicity. Although SiO 2 NPs are known to cause damage to the respiratory system, little is known about their oral toxicity, and their potential harm to the reproductive system is unclear. In this study, we used a Caenorhabditis elegans model to clarify SiO 2 NPs oral toxicity in vivo and explore their effect on the reproductive system. We exposed C. elegans to 0.25, 0.5 and 1 mg /mL SiO 2 NPs for 24 hr. Our results showed that SiO 2 NPs exposure for 24 hr did not affect nematode survival rates, but did affect, to varying degrees, the reproduction, development, and movement of nematodes, with nematode fecundity being the most sensitive to SiO 2 NPs toxicity. The NPs exposed group showed enhanced germ cell apoptosis and increased oxidative stress as seen through an increase in ROS and malondialdehyde (MDA), and decrease in reduced glutathione (GSH). N-acetyl-L-cysteine (NAC), an antioxidant, negated SiO 2 NPs effect on germ cells and restored nematodes reproductive ability. We also found that SiO 2 NPs could affect the expression of genes related to metal detoxification, oxidative stress, and apoptosis. The expression of metallothionein coding genes mtl-1 and mtl-2 changed most significantly among the tested genes. We demonstrated that SiO 2 NPs could enhance germ cell apoptosis by inducing oxidative stress, providing a new area for studies of the mechanism of SiO 2 NP toxicity.

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Section: Sio 2 Nps Enhanced Germ Cell Apoptosissupporting

confidence: 46%

Silica nanoparticles enhance germ cell apoptosis by inducing reactive oxygen species (ROS) formation in <i>Caenorhabditis elegans</i>

et al. 2020

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“…The characterization of SiNPs was performed, which is similar to our previous study (10). The results of Zeta potential and surface area of the SiNPs in the ultrapure water, DMEM, and saline are shown in Table 1.…”

Section: Characterization Of the Silica Nanoparticlesmentioning

confidence: 79%

“…Our previous study found that SiNPs could change the miRNA pro le of the spermatocytes exposed to the SiNPs for 30 generations and lead to the expression changes of 15 miRNAs, including 5 upregulated miRNAs and 10 downregulated miRNAs. Among the 15 miRNAs, the expressions of the miRNA-450b-3p were downregulated (10). The miRNA database prediction tool Starbase was used to predict the relationships between the miR-450b-3p and MTCH2 (21,28), which showed that the target gene of the miR-450-3p is MTCH2.…”

Section: Discussionmentioning

confidence: 99%

“…Exposure to Silica nanoparticles also exerted altered the expression of apoptosisassociated proteins in HaCaT cells (18). Our Previous study showed that SiNPs altered the miRNA pro le, and there were ve upregulated miRNAs and ten down-regulated miRNAs in spermatocyte cells, and miR-138 and miR-2861 were related to the death receptor pathways of apoptosis (10). However, the underlying mechanisms behind some miRNA in SiNPs induced male reproductive toxicity are still unclear.…”

Section: Introductionmentioning

confidence: 96%

“…Previous studies have shown that SiNPs could penetrate the blood-testis barrier (8) and decrease semen quality and count of sperm in mice (9). However, many of these studies focused on the pathways and proteins related to apoptosis and autophagy (10,11), and there are relatively few studies that focus on the epigenetic regulations of the toxic mechanisms of SiNPs on reproduction.…”

Section: Introductionmentioning

confidence: 99%

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Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

Zhou

Liu

et al. 2020

Preprint

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Background: Silica nanoparticles (SiNPs) are found in environmental particulate matter and are proven to have adverse effects on fertility. The relationship and underlying mechanisms between miRNAs and apoptosis induced by SiNPs during spermatogenesis is currently ambiguous. Experimental design: The present study was designed to investigate the role of miRNA-450b-3p in the reproductive toxicity caused by SiNPs. In vivo, 40 male mice were randomly divided into control and SiNPs groups, 20 per group. The mice in the SiNPs group were administrated 20 mg/kg SiNPs by tracheal perfusion once every 5 days, for 35 days, and the control group were given the equivalent of a normal luminal saline. In vitro, spermatocyte cells were divided into 0 and 5 μg/mL SiNPs groups, after passaged for 30 generations, the GC-2spd cells in 5 μg/mL SiNPs groups were transfected with miRNA-450b-3p and its mimic and inhibitor. Results: In vivo, the results showed that SiNPs damaged tissue structures of testis, decreased the quantity and quality of the sperm, reduced the expression of miR-450b-3p, and increased the protein expressions of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, and Caspase-3 in the testis. In vitro, SiNPs obviously repressed the viability and increased the LDH level and apoptosis rate, decreased the levels of the miR-450b-3p, significantly enhanced the protein expressions of the MTCH2, BID, BAX, Cytochrome C, Caspase-9, Caspase-3; while the mimic of miR-450b-3p reversed the changes induced by SiNPs, but inhibitor further promoted the effects induced by SiNPs.Conclusion: The result suggested that SiNPs could induce the spermatocyte apoptosis by inhibiting the miR-450b-3p expression to target promoting the MTCH2 resulting in activating mitochondrial apoptotic signaling pathways in the spermatocyte cells.

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Molecular Impacts of Advanced Nanomaterials at Genomic and Epigenomic Levels

Shekh¹,

Ansari

Omidi

et al. 2023

Impact of Engineered Nanomaterials in Genomics and Epigenomics

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Silica nanoparticles induce spermatocyte cell apoptosis through microRNA-2861 targeting death receptor pathway

Cited by 20 publications

References 47 publications

Silica nanoparticles enhance germ cell apoptosis by inducing reactive oxygen species (ROS) formation in <i>Caenorhabditis elegans</i>

Silica nanoparticles enhance germ cell apoptosis by inducing reactive oxygen species (ROS) formation in <i>Caenorhabditis elegans</i>

Silica nanoparticles inducing the apoptosis of spermatocyte cell through microRNA-450b-3p targeting MTCH2-mediating mitochondrial signaling pathway

Molecular Impacts of Advanced Nanomaterials at Genomic and Epigenomic Levels

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