Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli

Mathelié‐Guinlet, Marion; Cohen–Bouhacina, Touria; Gammoudi, Ibtissem; Martin, Axel; Béven, Laure; Delville, Marie‐Hélène; Grauby–Heywang, Christine

doi:10.1016/j.snb.2019.03.144

Cited by 68 publications

(33 citation statements)

References 30 publications

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“…Yun et al [76] prepararon una plataforma de biosensores de fluorescencia mejorada con metal (MEF) sobre sustratos fibrosos utilizando una capa de sílice que actuó como espaciador entre las nanopartículas de plata y las moléculas fluorescentes para optimizar el efecto MEF. En cuento a bacterias patógenas su detección es de importancia crítica para cuestiones de salud y seguridad en el diagnóstico clínico, la industria alimentaria, la calidad del agua y el medio ambiente, así como la lucha contra el posible bioterrorismo [77], Guinlet et al [78] presentaron resultados sobre el diseño de un biosensor electroquímico, en el que la presencia de NPs, funcionalizados con anticuerpos policlonales específicos (Abs), se utiliza para apuntar y unirse a la bacteria E. coli para su detección y cuantificación.…”

Section: Biosensoresunclassified

Obtención, funcionalización y aplicaciones biomédicas de las Nanopartículas de Sílice Mesoporosa: una revisión

Hincapie

Hernández

González

et al. 2020

DYNA

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Recientemente, las nanopartículas inorgánicas han sido ampliamente investigadas como agentes terapéuticos en los campos biomédicos debido a sus propiedades físicas, químicas y biológicas excepcionales, su fácil funcionalización superficial, su capacidad de carga y su excelente biocompatibilidad. El creciente aumento en investigaciones centradas en nanopartículas inorgánicas en especial las de sílice ha permitido entender su comportamiento en aplicaciones biomédicas, debido a que es un biomaterial con alta resistencia mecánica, bioactividad y reabsorbilidad mejoradas. En este artículo, se realiza la revisión de estudios recientes basados en Nanopartículas de Sílice Mesoporosa (MSN) cuyo enfoque principal se centra en diferentes métodos de síntesis, funcionalización y recubrimiento de superficie y algunas aplicaciones biomédicas como liberación controlada de fármacos e imágenes diagnósticas. Finalmente, se presentan los impactos de las modificaciones químicas de la superficie de las nanopartículas en cuanto a su biocompatibilidad y las diversas aplicaciones biomédicas debido a sus destacables características.

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Section: Biosensoresunclassified

Obtención, funcionalización y aplicaciones biomédicas de las Nanopartículas de Sílice Mesoporosa: una revisión

Hincapie

Hernández

González

et al. 2020

DYNA

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“…The need for real-time detection has been a major driving force behind the emergence of biosensors as promising alternative platforms capable of rapid, sensitive and potentially cost-attractive detection of pathogenic bacteria [ 9 ]. Great effort has gone into developing new biosensors targeting E. coli [ 10 , 11 , 12 , 13 , 14 , 15 ] and limits of detection (LOD) as low as 1 CFU/mL have been reported for the detection of E. coli using different types of biosensors [ 16 , 17 , 18 ]. Despite the progress and the low LODs achieved in recent years, there is still no practical biosensor which could overall satisfy the market requirements, such as short analysis time, high sensitivity and aptitude for detection in complex media.…”

Section: Introductionmentioning

confidence: 99%

Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium

et al. 2021

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A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of Escherichia coli in liquid media. The geometry of the biosensor consists of a GaAs membrane coated with a thin film of piezoelectric ZnO on its top surface. A pair of electrodes deposited on the ZnO film allows the generation of BAWs by lateral field excitation. The back surface of the membrane is functionalized with alkanethiol self-assembled monolayers and antibodies against E. coli. The antibody immobilization was investigated as a function of the concentration of antibody suspensions, their pH and incubation time, designed to optimize the immunocapture of bacteria. The performance of the biosensor was evaluated by detection tests in different environments for bacterial suspensions ranging between 103 and 108 CFU/mL. A linear dependence between the frequency response and the logarithm of E. coli concentration was observed for suspensions ranging between 103 and 107 CFU/mL, with the limit of detection of the biosensor estimated at 103 CFU/mL. The 5-fold regeneration and excellent selectivity towards E. coli detected at 104 CFU/mL in a suspension tinted with Bacillus subtilis at 106 CFU/mL illustrate the biosensor potential for the attractive operation in complex biological media.

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“…For the aforementioned reasons, the silica nanoparticles-based SPCE is seen to have great potential for use in the electrochemical detection of heavy metal ions, including As(III), in the environmental field. It is imperative to note that silica nanoparticles (SiNPs) have been established as one of the most promising support elements due to their high surface area, biocompatibility, and ease of functionalization that can further enhance the sensor performance [25]. Therefore, this work demonstrates the fabrication of SiNPs/SPCE-modified electrodes to narrow the gap of the unexplored strategy in improving the electrochemical detection of As(III) ions via the linear sweep anodic stripping voltammetry (LSASV) technique.…”

Section: Introductionmentioning

confidence: 99%

“…The SPCE was modified with 3-aminopropyltriethoxysilane (APTES)-functionalized SiNPs through the drop-casting method, and subsequently applied as an electrochemical interface, as illustrated in Figure 1. Through this simple work-up process, the high specificity and sensitivity of the sensor can be achieved from the abundant amino (NH 2 )-containing active groups on the electrode surface, which are favorable for the LSASV determination of As(III) [25]. The proposed scheme can be introduced as a new route for a simple, fast, and practical method in the advancement of sensor studies from the existing scheme, in which the latter requires complicated fabrication protocols using high-cost materials.…”

Section: Introductionmentioning

confidence: 99%

“…Materials 2020, 13, x FOR PEER REVIEW 3 of 15 active groups on the electrode surface, which are favorable for the LSASV determination of As(III) [25]. The proposed scheme can be introduced as a new route for a simple, fast, and practical method in the advancement of sensor studies from the existing scheme, in which the latter requires complicated fabrication protocols using high-cost materials.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Detection of Arsenite Using a Silica Nanoparticles-Modified Screen-Printed Carbon Electrode

et al. 2020

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Arsenic poisoning in the environment can cause severe effects on human health, hence detection is crucial. An electrochemical-based portable assessment of arsenic contamination is the ability to identify arsenite (As(III)). To achieve this, a low-cost electroanalytical assay for the detection of As(III) utilizing a silica nanoparticles (SiNPs)-modified screen-printed carbon electrode (SPCE) was developed. The morphological and elemental analysis of functionalized SiNPs and a SiNPs/SPCE-modified sensor was studied using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). The electrochemical responses towards arsenic detection were measured using the cyclic voltammetry (CV) and linear sweep anodic stripping voltammetry (LSASV) techniques. Under optimized conditions, the anodic peak current was proportional to the As(III) concentration over a wide linear range of 5 to 30 µg/L, with a detection limit of 6.2 µg/L. The suggested approach was effectively valid for the testing of As(III) found within the real water samples with good reproducibility and stability.

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Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli

Cited by 68 publications

References 30 publications

Obtención, funcionalización y aplicaciones biomédicas de las Nanopartículas de Sílice Mesoporosa: una revisión

Obtención, funcionalización y aplicaciones biomédicas de las Nanopartículas de Sílice Mesoporosa: una revisión

Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium

Electrochemical Detection of Arsenite Using a Silica Nanoparticles-Modified Screen-Printed Carbon Electrode

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