Silica Nanoparticles as Promising Drug/Gene Delivery Carriers and Fluorescent Nano-Probes: Recent Advances

Liu, Yiyao; Lou, Changchun; Yang, Hong; Shi, Mengran; Miyoshi, Hirokazu

doi:10.2174/156800911794328411

Cited by 38 publications

(32 citation statements)

References 48 publications

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“…CeO 2 , a rare-earth lanthanide element oxide, is mainly used in slurries for silicon wafer planarization [1–2], as automotive fuel additives to improve the efficiency of combustion [3–4], and as automobile catalytic converters [5]. SiO 2 nanoparticles are employed in the fabrication of electric and thermal insulators [6], as drug-delivery systems in nanomedicine [7–8], as anticaking and thickener agents in food production [9–10], as well as in cosmetics, drugs and printer toners [11]. …”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

Strobel¹,

Förster²,

Hilger³

2014

Beilstein J. Nanotechnol.

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SummaryCerium dioxide (CeO2) and silicon dioxide (SiO2) nanoparticles are of widespread use in modern life. This means that human beings are markedly exposed to them in their everyday life. Once passing biological barriers, these nanoparticles are expected to interact with endothelial cells, leading to systemic alterations with distinct influences on human health. In the present study we observed the metabolic impact of differently sized CeO2 (8 nm; 35 nm) and SiO2 nanoparticles (117 nm; 315 nm) on immortalized human microvascular (HMEC-1) and primary macrovascular endothelial cells (HUVEC), with particular focus on the CeO2 nanoparticles. The characterization of the CeO2 nanoparticles in cell culture media with varying serum content indicated a steric stabilization of nanoparticles due to interaction with proteins. After cellular uptake, the CeO2 nanoparticles were localized around the nucleus in a ring-shaped manner. The nanoparticles revealed concentration and time, but no size-dependent effects on the cellular adenosine triphosphate levels. HUVEC reacted more sensitively to CeO2 nanoparticle exposure than HMEC-1. This effect was also observed in relation to cytokine release after nanoparticle treatment. The CeO2 nanoparticles exhibited a specific impact on the release of diverse proteins. Namely, a slight trend towards pro-inflammatory effects, a slight pro-thrombotic impact, and an increase of reactive oxygen species after nanoparticle exposure were observed with increasing incubation time. For SiO2 nanoparticles, concentration- and time-dependent effects on the metabolic activity as well as pro-inflammatory reactions were detectable. In general, the effects of the investigated nanoparticles on endothelial cells were rather insignificant, since the alterations on the metabolic cell activity became visible at a nanoparticle concentration that is by far higher than those expected to occur in the in vivo situation (CeO2 nanoparticles: 100 µg/mL; SiO2 nanoparticles: 10 µg/mL).

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Section: Introductionmentioning

confidence: 99%

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

Strobel¹,

Förster²,

Hilger³

2014

Beilstein J. Nanotechnol.

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“…This has been attempted by several groups. [22][23][24][25] Although few research groups have concentrated on how nanoparticles adhere to targeted cell surfaces, attempts have been directed towards to interactions between targeted nanoparticles and endothelial cells under hemodynamic shear flow conditions. 15,26 In this study, synthesis of Fe 3 O 4 @SiO 2 (FITC)-NH 2 nanoparticles was based on sol-gel growth of silica in a limited domain of water in water-in-oil microemulsion.…”

Section: Resultsmentioning

confidence: 99%

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Yang¹,

Zhao²,

Liu³

et al. 2013

IJN

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Multifunctional nanomaterials with unique magnetic and luminescent properties have broad potential in biological applications. Because of the overexpression of vascular cell adhesion molecule-1 (VCAM-1) receptors in inflammatory endothelial cells as compared with normal endothelial cells, an anti-VCAM-1 monoclonal antibody can be used as a targeting ligand. Herein we describe the development of multifunctional core-shell Fe 3 O 4 @SiO 2 nanoparticles with the ability to target inflammatory endothelial cells via VCAM-1, magnetism, and fluorescence imaging, with efficient magnetic resonance imaging contrast characteristics. Superparamagnetic iron oxide and fluorescein isothiocyanate (FITC) were loaded successfully inside the nanoparticle core and the silica shell, respectively, creating VCAM-1-targeted Fe 3 O 4 @ SiO 2 (FITC) nanoparticles that were characterized by scanning electron microscopy, transmission electron microscopy, fluorescence spectrometry, zeta potential assay, and fluorescence microscopy. The VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles typically had a diameter of 355 ± 37 nm, showed superparamagnetic behavior at room temperature, and cumulative and targeted adhesion to an inflammatory subline of human umbilical vein endothelial cells (HUVEC-CS) activated by lipopolysaccharide. Further, our data show that adhesion of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles to inflammatory HUVEC-CS depended on both shear stress and duration of exposure to stress. Analysis of internalization into HUVEC-CS showed that the efficiency of delivery of VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles was also significantly greater than that of nontargeted Fe 3 O 4 @SiO 2 (FITC)-NH 2 nanoparticles. Magnetic resonance images showed that the superparamagnetic iron oxide cores of the VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles could also act as a contrast agent for magnetic resonance imaging. Taken together, the cumulative adhesion and uptake potential of these VCAM-1-targeted Fe 3 O 4 @SiO 2 (FITC) nanoparticles targeted to inflammatory endothelial cells could be used in the transfer of therapeutic drugs/genes into these cells or for diagnosis of vascular disease at the molecular and cellular levels in the future.

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“…Silica coating of MNPs has been used to reduce oxidation of the magnetic core, which is a primary reason for loss of transverse relaxivity of the particles [78]. The silica shell can protect the metallic core from oxidation under aqueous conditions and silanol groups enable reaction with alcohols to provide stability in nonaqueous media and provide a platform for covalent linkage to numerous specific ligands [85]. Several methods have been reported to immobilize mesoporous silica onto the MNP surface facilitating drug delivery and imaging [86,87].…”

Section: Formulation Developments and Product Synthesismentioning

confidence: 99%

Formulation Design Facilitates Magnetic Nanoparticle Delivery to Diseased Cells and Tissues

et al. 2014

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Magnetic nanoparticles (MNPs) accumulate at disease sites with the aid of magnetic fields; biodegradable MNPs can be designed to facilitate drug delivery, influence disease diagnostics, facilitate tissue regeneration and permit protein purification. Because of their limited toxicity, MNPs are widely used in theranostics, simultaneously facilitating diagnostics and therapeutics. To realize therapeutic end points, iron oxide nanoparticle cores (5–30 nm) are encapsulated in a biocompatible polymer shell with drug cargos. Although limited, the toxic potential of MNPs parallels magnetite composition, along with shape, size and surface chemistry. Clearance is hastened by the reticuloendothelial system. To surmount translational barriers, the crystal structure, particle surface and magnetic properties of MNPs need to be optimized. With this in mind, we provide a comprehensive evaluation of advancements in MNP synthesis, functionalization and design, with an eye towards bench-to-bedside translation.

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Silica Nanoparticles as Promising Drug/Gene Delivery Carriers and Fluorescent Nano-Probes: Recent Advances

Cited by 38 publications

References 48 publications

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro

Formulation Design Facilitates Magnetic Nanoparticle Delivery to Diseased Cells and Tissues

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