Silica-chitosan hybrid support for laccase immobilization

Girelli, Anna Maria; Quattrocchi, Luisa; Scuto, Francesca Romana

doi:10.1016/j.jbiotec.2020.05.004

Cited by 18 publications

(10 citation statements)

References 20 publications

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“…Various solid supports have been used for immobilization of laccase including materials of various origin and chemical composition such as silica and inorganic materials ( Girelli et al, 2020 ), chitosan ( Bilal et al, 2019b ), and metal oxides ( Zdarta et al, 2018a ). The extent of laccase immobilization on these solid supports depends upon their properties such as chemical composition, surface area and functional groups on the surface ( Zdarta et al, 2018a ).…”

Section: Introductionmentioning

confidence: 99%

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Adamian

Lonappan

Alokpa

et al. 2021

Front. Bioeng. Biotechnol.

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Τhe ligninolytic enzyme laccase has proved its potential for environmental applications. However, there is no documented industrial application of free laccase due to low stability, poor reusability, and high costs. Immobilization has been considered as a powerful technique to enhance laccase’s industrial potential. In this technology, appropriate support selection for laccase immobilization is a crucial step since the support could broadly affect the properties of the resulting catalyst system. Through the last decades, a large variety of inorganic, organic, and composite materials have been used in laccase immobilization. Among them, carbon-based materials have been explored as a support candidate for immobilization, due to their properties such as high porosity, high surface area, the existence of functional groups, and their highly aromatic structure. Carbon-based materials have also been used in culture media as supports, sources of nutrients, and inducers, for laccase production. This study aims to review the recent trends in laccase production, immobilization techniques, and essential support properties for enzyme immobilization. More specifically, this review analyzes and presents the significant benefits of carbon-based materials for their key role in laccase production and immobilization.

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Section: Introductionmentioning

confidence: 99%

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Adamian

Lonappan

Alokpa

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

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“…Traditional carriers include inorganic, organic and composite materials such as activated carbon (AC), clay, chitosan, agar, and silica. Mineral materials are common inorganic carriers with low cost and easy accessibility, excellent thermal and chemical stability, and a high number of adsorption sites [ 61 ]. Among them, carbon-based materials have great potential for practical applications owing to their high thermal and chemical stability [ 62 ].…”

Section: Overview Of Immobilization Technologymentioning

confidence: 99%

“…For example, considering the high biocompatibility and excellent stability of organic carriers, as well as the stability and chemical inertness of inorganic carriers, the combination of these two materials to obtain composite-carrier materials has been used to develop more efficient carriers. Girelli et al [ 61 ] demonstrated that immobilizing laccase in silica–chitosan composites as carriers had higher thermal stability, and immobilized laccase maintained 40% of its enzyme activity after 200 days. Therefore, this is noteworthy for the study of stable performance, high biocompatibility, and affordable composite-carrier materials.…”

Section: Overview Of Immobilization Technologymentioning

confidence: 99%

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Liu

Yang

Zhang

et al. 2022

IJERPH

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In the field of environmental science and engineering, microorganisms, enzymes and algae are promising biomass materials that can effectively degrade pollutants. However, problems such as poor environmental adaptability, recycling difficulties, and secondary pollution exist in the practical application of non-immobilized biomass materials. Biomass immobilization is a novel environmental remediation technology that can effectively solve these problems. Compared with non-immobilized biomass, immobilized biomass materials have the advantages of reusability and stability in terms of pH, temperature, handling, and storage. Many researchers have studied immobilization technology (i.e., methods, carriers, and biomass types) and its applications for removing refractory organic pollutants. Based on this, this paper reviews biomass immobilization technology, outlines the mechanisms and factors affecting the removal of refractory organic pollutants, and introduces the application of immobilized biomass materials as fillers for reactors in water purification. This review provides some practical references for the preparation and application of immobilized biomass materials and promotes further research and development to expand the application range of this material for water purification.

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“…To overcome those disadvantages, immobilized enzymes for their satisfactory reusability have attracted heightened attention 1‐3 . Various carriers including, but not limited to, porous SiO 2 , 4‐6 inorganic oxides, 7,8 magnetic particles, 9‐13 and natural polymers, 14‐16 were applied for enzymes immobilization. However, the limited immobilized enzymes amount and loss of the enzyme activity hinder the development of immobilized enzymes.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of post‐modified poly(ester‐amino) microspheres via aza‐Michael precipitation polymerization and its use for enzyme immobilization

Sun

Jiang

et al. 2021

Polymers for Advanced Techs

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Uniform poly(ester‐amino) microspheres (PEAs) were prepared by catalyst‐free aza‐Michael addition precipitation polymerization of trihydroxymethylpropyl triacrylate (TMPTA) and piperazine (PA) in acetonitrile. The effects of monomer feed ratio and concentration, polymerization temperature, and time on the size and morphology of PEAs were investigated. Monodisperse PEAs with size about 4 μm were successfully obtained at 2/3 molar ratio of TMPTA/PA and 4°C. The chemical structure of PEAs was characterized. There were amount of unreacted acrylate groups remained on the surface of PEAs prepared at the imino/acrylate molar ratio of 1/1.1, which provide lots of active sites for further chemical modification, especially for bioactivator immobilization. Laccase was used as an example to determine bio‐immobilization activity of PEAs. Immobilized laccase exhibited higher tolerance to pH and temperature. The immobilized laccase was used as biocatalyst for degradation of Malachite Green. About 53% of its initial catalytic activity was retained after 4 cycles of reuse.

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Silica-chitosan hybrid support for laccase immobilization

Cited by 18 publications

References 20 publications

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Synthesis of post‐modified poly(ester‐amino) microspheres via aza‐Michael precipitation polymerization and its use for enzyme immobilization

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