Silibinin Inhibits Proliferation, Induces Apoptosis and Causes Cell Cycle Arrest in Human Gastric Cancer MGC803 Cells Via STAT3 Pathway Inhibition

Wang, Yixin; Cai, Hong; Ji, Gang; Zhou, Tiancai; Wu, Hai

doi:10.7314/apjcp.2014.15.16.6791

Cited by 50 publications

(30 citation statements)

References 74 publications

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“…Upon mitogenic stimulation, D-type cyclins, such as cyclin D1, are induced, and subsequently bind to and activate CDK4 and CDK6. These cyclin D-dependent kinases then initiate the phosphorylation of Rb, relieving the inhibition of E2F-1 and allowing for the expression of specific E2F-1 target genes (18). In the present study, Fan suppressed the expression of cyclin D1, CDK4 and CDK6, suggesting that Fan successfully blocked the cell cycle progression of SPC-A-1 lung cancer cells.…”

Section: Discussionsupporting

confidence: 52%

Fangchinoline inhibits the proliferation of SPC-A-1 lung cancer cells by blocking cell cycle progression

Luo

Jianming

et al. 2015

Experimental and Therapeutic Medicine

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Fangchinoline (Fan) is a bioactive compound isolated from the Chinese herb S. Moore (Fen Fang Ji). The aim of the present study was to investigate the effect of Fan on the proliferation of SPC-A-1 lung cancer cells, and to define the associated molecular mechanisms. Following treatment with Fan, Cell Counting Kit-8, phase contrast imaging and Giemsa staining assays were used to detect cell viability; flow cytometry was performed to analyze the cell cycle distribution; and reverse transcription-quantitative polymerase chain reaction and western blot assays were used to investigate changes in the expression levels of cell cycle-associated genes and proteins. In the present study, treatment with Fan markedly inhibited the proliferation of SPC-A-1 lung cancer cells and significantly increased the percentage of cells in the G/G phase of the cell cycle in a dose-dependent manner (P<0.05 for 2.5-5 µm; P<0.01 for 10 µm), whereas the percentage of cells in the S and G/M phases were significantly reduced following treatment (P<0.05 for 5 µm; P<0.01 for 10 µm). Mechanistically, Fan significantly reduced the mRNA expression levels of cyclin D1, cyclin-dependent kinase 4 (CDK4) and CDK6 (P<0.05 for 2.5-5 µm; P<0.01 for 10 µm), which are key genes in the regulation of the G/G phase of the cell cycle. Furthermore, treatment with Fan also decreased the expression of phosphorylated retinoblastoma (Rb) and E2F transcription factor-1 (E2F-1) proteins (P<0.05 for 5 µm; P<0.01 for 10 µm). In summary, the present study demonstrated that Fan inhibited the proliferation of SPC-A-1 lung cancer cells and induced cell cycle arrest at the G/G phase. These effects may be mediated by the downregulation of cellular CDK4, CDK6 and cyclin D1 levels, thus leading to hypophosphorylation of Rb and subsequent suppression of E2F-1 activity. Therefore, the present results suggest that Fan may be a potential drug candidate for the prevention of lung cancer.

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Section: Discussionsupporting

confidence: 52%

Fangchinoline inhibits the proliferation of SPC-A-1 lung cancer cells by blocking cell cycle progression

Luo

Jianming

et al. 2015

Experimental and Therapeutic Medicine

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“…23), a marker of poor prognosis for many cancers, including breast (24). In a preclinical model of human gastric cancer, Wang and colleagues reported that the mechanism of action of silybin was related to a specific suppression of pSTAT3 and downregulation of STAT3 target genes, including Mcl-1, Bcl-xL, and survivin (25). A future clinical trial might explore silybin activity as a pSTAT3 inhibitor in breast cancer patients.…”

Section: Discussionmentioning

confidence: 99%

A Presurgical Study of Oral Silybin-Phosphatidylcholine in Patients with Early Breast Cancer

Lazzeroni

Guerrieri-Gonzaga

Gandini

et al. 2016

Cancer Prevention Research

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“…Today, the biological characteristics of gastric cancer cells are investigated mainly through studying proliferation (Kato et al, 2012), apoptosis (Akagi et al, 2013), nude mice tumorigenic (Zheng et al, 2010) and protein molecular level of gastric cancer cells (Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian¹,

Li²,

Jia³

et al. 2016

Asian Pacific Journal of Cancer Prevention

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Silibinin Inhibits Proliferation, Induces Apoptosis and Causes Cell Cycle Arrest in Human Gastric Cancer MGC803 Cells Via STAT3 Pathway Inhibition

Cited by 50 publications

References 74 publications

Fangchinoline inhibits the proliferation of SPC-A-1 lung cancer cells by blocking cell cycle progression

Fangchinoline inhibits the proliferation of SPC-A-1 lung cancer cells by blocking cell cycle progression

A Presurgical Study of Oral Silybin-Phosphatidylcholine in Patients with Early Breast Cancer

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

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