Silibinin In Vitro Protects A549 Cells from Staphylococcus aureus-Mediated Injury and In Vivo Alleviates the Lung Injury of Staphylococcal Pneumonia

Wang, Xin; Dong, Jing; Dai, Xianzhong; Wang, Jianfeng; Li, Hongen; Lu, Chongjian; Tan, Winson Jianhong; Gao, Xiaohan; Shi-jin, BU; Niu, Xiaodi

doi:10.1055/s-0032-1328068

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…aureus (Table ). For example, flavones, including quercetin, farrerol, isorhamnetin, lysionotin, phloretin, diosmetin, silibinin, apigenin, and chalcone, inhibit the production of α-hemolysin by S. aureus.…”

Section: α-Hemolysinmentioning

confidence: 99%

Natural Products That Target Virulence Factors in Antibiotic-Resistant Staphylococcus aureus

Liu

Zhu

et al. 2019

J. Agric. Food Chem.

114

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The increase in the incidence of antibiotic-resistant Staphylococcus aureus (S. aureus) associated infections necessitates the urgent development of novel therapeutic strategies and antibacterial drugs. Antivirulence strategy is an especially compelling alternative strategy due to its low selective pressure for the development of drug resistance in bacteria. Plants and microorganisms are not only important food and medicinal resources but also serve as sources for the discovery of natural products that target bacterial virulence factors. This review discusses the mechanisms of the major virulence factors of S. aureus, including the accessory gene regulator quorum-sensing system, bacterial biofilm formation, α-hemolysin, sortase A, and staphyloxanthin. We also provide an overview of natural products isolated from plants and microorganisms with activity against the major virulence factors of S. aureus and their adjuvant effects on existing antibiotics to overcome antibiotic-resistant S. aureus. Finally, the limitations and solutions of these antivirulence compounds are discussed, which will help in the development of novel antibacterial drugs against antibiotic-resistant S. aureus.

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Section: α-Hemolysinmentioning

confidence: 99%

Natural Products That Target Virulence Factors in Antibiotic-Resistant Staphylococcus aureus

Liu

Zhu

et al. 2019

J. Agric. Food Chem.

114

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“…Interestingly, we recently discovered that silibinin effectively inhibited the expression of the α‐toxin of Staphylococcus aureus and alleviated α‐toxin‐induced lung cell injury, suggesting that silibinin was an effective antivirulent agent (Wang et al . ). However, the antivirulence efficacy of silibinin to S. suis infection and the underlying mechanisms of action remain to be elucidated.…”

Section: Introductionmentioning

confidence: 97%

Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin

Shen

Liu

et al. 2018

J Appl Microbiol

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Aims:To determine the antivirulence properties of silibinin against suilysin (SLY), a virulence factor of Streptococcus suis serotype 2 (SS2) that plays an important role in the pathogenesis of S. suis infection and its protective effect against SS2 infection in a mouse model. Methods and Results: Susceptibility testing, haemolysis assay and Western blot assays were employed to evaluate the performance of silibinin on SLY poreforming activity. Cytotoxicity assays and mouse infection tests were also performed to determine the efficacy of silibinin against SS2 infection. The results showed that silibinin, a flavonoid with little anti-S. suis activity, was identified to be a potent antagonist of SLY-mediated haemolysis through the inhibition of its oligomerization. Treatment with silibinin reduced S. suisinduced cytotoxicity in macrophages (J774 cells). In addition, S. suis-infected mice that received silibinin showed a lower bacterial burden. Conclusions: Our results demonstrated that silibinin is a promising candidate for the development of antivirulence therapeutic agents to treat S. suis infections. Significance and Impact of the Study: The antivirulent property of silibinin against SS2 by targeting SLY provides the possibility for the future pharmaceutical application of silibinin to prevent and treat S. suis infection.

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“…Qiu et al studied on isoalantolactone a natural compound from Inula helenium (IAL) against S. aureus in vitro and reported that IAL can markedly inhibit the expression of α -toxin at very low concentrations [ 51 ]. In other studies about α -toxin, it is stated that capsaicin and silibinin have prevented α -toxin-mediated alveolar cell injury through animal studies about pneumonia caused by S. aureus [ 52 , 53 ].…”

Section: Pathogensmentioning

confidence: 99%

Future Directions and Molecular Basis of Ventilator Associated Pneumonia

Aykaç

Özsürekçi

Başaranoğlu

2017

Canadian Respiratory Journal

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Mechanical ventilation is a lifesaving treatment and has complications such as ventilator associated pneumonia (VAP) that lead to high morbidity and mortality. Moreover VAP is the second most common hospital-acquired infection in pediatric intensive care units. Although it is still not well understood, understanding molecular pathogenesis is essential for preventing and treating pneumonia. A lot of microbes are detected as a causative agent of VAP. The most common isolated VAP pathogens in pediatric patients are Staphylococcus aureus, Pseudomonas aeruginosa, and other gram negative bacteria. All of the bacteria have different pathogenesis due to their different virulence factors and host reactions. This review article focused on mechanisms of VAP with molecular pathogenesis of the causative bacteria one by one from the literature. We hope that we know more about molecular pathogenesis of VAP and we can investigate and focus on the management of the disease in near future.

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Silibinin In Vitro Protects A549 Cells from Staphylococcus aureus-Mediated Injury and In Vivo Alleviates the Lung Injury of Staphylococcal Pneumonia

Cited by 10 publications

References 30 publications

Natural Products That Target Virulence Factors in Antibiotic-Resistant Staphylococcus aureus

Natural Products That Target Virulence Factors in Antibiotic-Resistant Staphylococcus aureus

Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin

Future Directions and Molecular Basis of Ventilator Associated Pneumonia

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