Silencing profilin-1 inhibits gastric cancer progression<i>via</i>integrin β1/focal adhesion kinase pathway modulation

Cheng, Yajun; Zhu, Zhenxin; Zhou, Jing; Hu, Zunqi; Zhang, Jianpeng; Cai, Qing-Qing; Wang, Lianghua

doi:10.3748/wjg.v21.i8.2323

Cited by 44 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, ITGB1 could combine with the FN, subsequently recruit and activate the tyrosine protein kinase FAK, which regulates cell proliferation and apoptosis (Liao, Zhang, Zhao, & Liu, 2018;Park, Ryu, & Han, 2011). Meanwhile, the catalytic subunit of PI3K is transported to the membrane when FAK binds to the SH2 domain of PI3K and catalyse the phosphorylation of AKT (Hwangbo, Kim, Lee, & Lee, 2010), and the ITGB1/FAK/PI3K/Akt pathway is an important signalling pathway involved in cell growth and survival (Cheng et al, 2015). Furthermore, the PI3K/AKT activation is also induced by the E-cadherin in epithelial ovarian cancer cells (De Santis, Miotti, Mazzi, Canevari, & Tomassetti, 2009 Additionally, the ROS level induced by P53 (Li, Dietz, & Harsdorf, 1999) was reduced in the oocyte of 3D culture, which also indicated the oocyte quality was improved.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A new three‐dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (Bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells

Shen

Wang

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

At present, many three‐dimensional (3D) culture systems have been reported, improving the oocyte quality of in vitro maturation (IVM), yet the mechanism still needs to be further explored. Here we examined the effects of a new self‐made 3D glass scaffold on buffalo oocyte maturation; meanwhile, the underlying mechanism on buffalo oocyte maturation was also detected. Compared to the two‐dimensional (2D) glass dish culture, results revealed that the 3D culture can improve the first polar body rate of oocytes, subsequent cleavage and blastocysts rate of parthenogenetic activation embryos (p < .05). The extracellular matrix‐related proteins COL1A1, COL2A1, COL3A1, FN and cell connection‐related proteins N‐cadherin, E‐cadherin, GJA1 were found higher in cumulus cells of 3D culture. Moreover, in cumulus cells, proteins of the PI3K/AKT pathway reported being regulated by FN and E‐cadherin including PI3K P85 and p‐AKT were also higher in 3D culture. Furthermore, proapoptosis proteins P53, BAX, caspase‐3 were lower in both cumulus cells and oocytes in 3D culture, while proteins PCNA and BCL2 showed the opposite result. Results also showed that the apoptosis was inhibited, and the proliferation was enhanced in cumulus cells of 3D culture. Finally, the cumulus expansion‐related genes HAS2, CD44, HMMR, PTX3, PTGS2 were found higher in cumulus cells of 3D culture. Taken together, the 3D culture could promote oocyte maturation by regulating proteins correlated with the ECM, cell connection and PI3K/AKT pathway, inhibiting the apoptosis of cumulus cells and oocytes, enhancing the proliferation of cumulus cells and the cumulus expansion.

show abstract

Section: Discussionmentioning

confidence: 99%

“…It is well known that cumulus expansion has a positive relationship with the quality of oocyte maturation. Cumulus expansion regulated by ERK1/2 (Fan et al, 2009) is a component of the ITGB1/FAK signal pathway (Cheng et al, 2015). Meanwhile, research also shows that the activities of ERK1/2 rely on PCK activities (Prochazka & Nemcova 2019).…”

Section: Discussionmentioning

confidence: 99%

A new three‐dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (Bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells

Shen

Wang

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…3 Like many other cancer types, initiation and progression of gastric cancer involves multistep processes of various genetic and molecular factors, including activation of multiple oncogenes and suppression of tumor suppressor genes. 4,5 Molecular pathways that affect cell proliferation and migration, including the integrin/focal adhesion kinase, 6 mammalian target of rapamycin (mTOR) signaling, 7 mitogen-activated protein kinases (MAPK), 8 and nuclear factor-jB (NF-jB) pathways, 9 have been demonstrated to play roles in development of gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

Tissue transglutaminase-1 promotes stemness and chemoresistance in gastric cancer cells by regulating Wnt/β-catenin signaling

Huang

Chen

2016

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Gastric cancer is a common malignancy, and is one of the most frequent causes of cancer deaths worldwide. Recently, members of the transglutaminases (TGM) family, especially TGM2, have been implicated in the progression and drug resistance of cancers, but the function of TGM1 in cancer development has been largely overlooked. In this study, we demonstrate the roles of TGM1 in development of gastric cancer. We found that expression levels of TGM1 were upregulated in both gastric cancer tissues and cultured gastric cancer cells, and that TGM1 expression levels were correlated with patient survival. In cultured gastric cancer cells, loss of TGM1 expression inhibited cell proliferation and promoted apoptosis, as well increased gastric cancer cell sensitivity to chemotherapeutic drugs and reducing stemness. These results strongly supported the participation of TGM1 in the regulation of gastric cancer development. In addition, we found evidence that the mechanism of action of TGM1 in regulating gastric cancer cell might involve the Wnt signaling pathway, as loss of TGM1 expression in gastric cancer cells led to a significant suppression of Wnt signaling activities.

show abstract

“…Profilin-1 (PFN1) Non-gastric cells Knockdown inhibits proliferation of gastric cancer cells by induction cell cycle arrest at G0/G1 phase (Cheng et al, 2015, Moens & Coumans, 2015.…”

Section: S Ribosomal Protein L13 (Rpl13)mentioning

confidence: 99%

“…Profilin-1 (PFN1) Gastric cancer and other cancers PFN1 plays an important role in gastric carcinoma progression (Cheng et al, 2015) and renal carcinoma (Minamida et al, 2011).…”

Section: Generation Of Precursor Metabolites and Energymentioning

confidence: 99%

Helicobacter pyloriouter inflammatory protein A (OipA) suppresses apoptosis of AGS gastric cells in vitro

Al-Maleki

Loke

Lui

et al. 2017

Cellular Microbiology

View full text Add to dashboard Cite

Outer inflammatory protein A (OipA) is an important virulence factor associated with gastric cancer and ulcer development; however, the results have not been well established and turned out to be controversial. This study aims to elucidate the role of OipA in Helicobacter pylori infection using clinical strains harbouring oipA "on" and "off" motifs. Proteomics analysis was performed on AGS cell pre-infection and postinfection with H. pylori oipA "on" and "off" strains, using liquid chromatography/mass spectrometry. AGS apoptosis and cell cycle assays were performed. Moreover, expression of vacuolating cytotoxin A (VacA) was screened using Western blotting. AGS proteins that have been suggested previously to play a role or associated with gastric disease were down-regulated postinfection with oipA "off" strains comparing to oipA "on" strains. Furthermore, oipA "off" and ΔoipA cause higher level of AGS cells apoptosis and G0/G1 cell-cycle arrest than oipA "on" strains. Interestingly, deletion of oipA increased bacterial VacA production. The capability of H. pylori to induce apoptosis and suppress expression of proteins having roles in human disease in the absence of oipA suggests that strains not expressing OipA may be less virulent or may even be protective against carcinogenesis compared those expressing OipA. This potentially explains the higher incidence of gastric cancer in East Asia where oipA "on" strains predominates.

show abstract

Silencing profilin-1 inhibits gastric cancer progressionviaintegrin β1/focal adhesion kinase pathway modulation

Abstract: These findings suggest that PFN1 plays a critical role in gastric carcinoma progression, and these effects are likely mediated through the integrin β1/FAK pathway.

Cited by 44 publications

References 36 publications

A new three‐dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (Bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells

A new three‐dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (Bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells

Tissue transglutaminase-1 promotes stemness and chemoresistance in gastric cancer cells by regulating Wnt/β-catenin signaling

Helicobacter pyloriouter inflammatory protein A (OipA) suppresses apoptosis of AGS gastric cells in vitro

Contact Info

Product

Resources

About