Silencing of XB130 Is Associated with Both the Prognosis and Chemosensitivity of Gastric Cancer

Shi, Min; Huang, Weizhen; Li, Lin; Zheng, Deyou; Zuo, Qiang; Wang, Lin; Wang, Na; Wu, Yajun; Liao, Yulin; Liao, Wangjun

doi:10.1371/journal.pone.0041660

Cited by 29 publications

(54 citation statements)

References 19 publications

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“…Further, nuclear expression of XB-130 was an independent prognostic factor of postoperative survival. Shi et al [13] has reported that both XB130 mRNA and protein expression were detectable in normal gastric tissues. The reduced XB130 protein expression is a prognostic biomarker for shorter survival and a higher recurrence rate in patients with GC, as well as for the response to chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical staining was carried out using the Dako Envision System (Dako, Carpinteria, CA) and rabbit polyclonal anti-XB130 antibody (1:100; OriGene) following the manufacturer's recommended protocol.The staining intensity and extent scores was according to the previous report [13]. Briefly, the staining intensity was scored as 0 (negative), 1 (weak), 2 (medium), or 3 (strong).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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XB130 expression in human osteosarcoma: a clinical and experimental study

Wang¹,

Ruiguo²,

Liu³

et al. 2015

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Identifying prognostic factors for osteosarcoma (OS) aids in the selection of patients who require more aggres sive management. XB130 is a newly characterized adaptor protein that was reported to be a prognostic factor of certain tumor types. However, the association between XB130 expression and the prognosis of OS remains unknown. In the present study, we investigated the association between XB130 expression and clinicopathologic features and prognosis in patients suffering OS, and further investigated its potential role on OS cells in vitro and vivo. A retrospective immunohistochemical study of XB130 was performed on archival formalin-fixed paraffin-embedded specimens from 60 pairs of osteosarcoma and noncancerous bone tissues, and compared the expression of XB130 with clinicopathological parameters. We then investigate the effect of XB130 sliencing on invasion in vitro and lung metastasis in vivo of the human OS cell line. Immunohistochemical assays revealed that XB130 expression in OS tissues was significantly higher than that in corresponding noncancerous bone tissues (P = 0.001). In addition, high XB130 expression more frequently occurred in OS tissues with advanced clinical stage (P = 0.002) and positive distant metastasis (P = 0.001). Moreover, OS patients with high XB130 expression had significantly shorter overall survival and disease-free survival (both P < 0.001) when compared with patients with the low expression of XB130. The univariate analysis and multivariate analysis shown that high XB130 expression and distant metastasis were the independent poor prognostic factor.We showed that XB130 depletion by RNA interference inhibited invasion of XB130-rich U2OS cells in vitro and lung metastasis in vivo. This is the first study to reveal that XB130 overexpression may be related to the prediction of metastasis potency and poor prognosis for OS patients, suggesting that XB130 may serve as a prognostic marker for the optimization of clinical treatments. Furthermore, XB130 is the potential molecular target for OS therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

XB130 expression in human osteosarcoma: a clinical and experimental study

Wang¹,

Ruiguo²,

Liu³

et al. 2015

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“…RNA sequence analysis by Illumina Body Map also shows expression in lymph node, adipocytes, adrenal gland, breast, ovary, prostate, and testis (http://www.genecard.org/) [8]. The expression of XB130 protein has also been confirmed with immunohistochemistry staining in human thyroid [14], submucosal glands of esophagus [15], and stomach [16]. …”

Section: Xb130: a Novel Gene And Proteinmentioning

confidence: 99%

XB130—A Novel Adaptor Protein: Gene, Function, and Roles in Tumorigenesis

et al. 2014

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Several adaptor proteins have previously been shown to play an important role in the promotion of tumourigenesis. XB130 (AFAP1L2) is an adaptor protein involved in many cellular functions, such as cell survival, cell proliferation, migration, and gene and miRNA expression. XB130's functional domains and motifs enable its interaction with a multitude of proteins involved in several different signaling pathways. As a tyrosine kinase substrate, tyrosine phosphorylated XB130 associates with the p85α regulatory subunit of phosphoinositol-3-kinase (PI3K) and subsequently affects Akt activity and its downstream signalling. Tumourigenesis studies show that downregulation of XB130 expression by RNAi inhibits tumor growth in mouse xenograft models. Furthermore, XB130 affects tumor oncogenicity by regulating the expression of specific tumour suppressing miRNAs. The expression level and pattern of XB130 has been studied in various human tumors, such as thyroid, esophageal, and gastric cancers, as well as, soft tissue tumors. Studies show the significant effects of XB130 in tumourigenesis and suggest its potential as a diagnostic biomarker and therapeutic target for cancer treatments.

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“…Although XB130 has not attracted significant attention until recent years, it has been established that XB130 is a comparatively widely expressed protein. A previous study identified that the mRNA is frequently expressed in human spleen and thyroid, and although it si relatively lower, its expression could also be detected in the kidney, brain, lung, pancreas, liver, colon and stomach (40). Additionally, different approaches using several cell lines showed that XB130 tends to be distributed in the cytoplasm.…”

Section: Xb130: a Newly Studied Adaptor Protein In Cancer Signal Tranmentioning

confidence: 98%

“…However, in contrast to the studies indicating a cancer promotion effect of XB130, Shi et al (40) found evidence suggesting that the XB130 mRNA and protein were constitutively expressed in normal gastric tissue, while relatively lower expressed in gastric cancer (GC) cells (40). The study analyzed survival or recurrence in 411 GC patients and noted that low XB130 expression predicted a lower survival and higher recurrence.…”

Section: Xb130 In Cancermentioning

confidence: 98%

XB130: A novel adaptor protein in cancer signal transduction

Zhang

et al. 2016

Biomedical Reports

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Abstract. Adaptor proteins are functional proteins that contain two or more protein-binding modules to link signaling proteins together, which affect cell growth and shape and have no enzymatic activity. The actin filament-associated protein (AFAP) family is an important member of the adaptor proteins, including AFAP1, AFAP1L1 and AFAP1L2/XB130. AFAP1 and AFAP1L1 share certain common characteristics and function as an actin-binding protein and a cSrc-activating protein. XB130 exhibits certain unique features in structure and function. The mRNA of XB130 is expressed in human spleen, thyroid, kidney, brain, lung, pancreas, liver, colon and stomach, and the most prominent disease associated with XB130 is cancer. XB130 has a controversial effect on cancer. Studies have shown that XB130 can promote cancer progression and downregulation of XB130-reduced growth of tumors derived from certain cell lines. A higher mRNA level of XB130 was shown to be associated with a better survival in non-small cell lung cancer. Previous studies have shown that XB130 can regulate cell growth, migration and invasion and possibly has the effect through the cAMP-cSrc-phosphoinositide 3-kinase/Akt pathway. Except for cancer, XB130 is also associated with other pathological or physiological procedures, such as airway repair and regeneration.

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Silencing of XB130 Is Associated with Both the Prognosis and Chemosensitivity of Gastric Cancer

Cited by 29 publications

References 19 publications

XB130 expression in human osteosarcoma: a clinical and experimental study

XB130 expression in human osteosarcoma: a clinical and experimental study

XB130—A Novel Adaptor Protein: Gene, Function, and Roles in Tumorigenesis

XB130: A novel adaptor protein in cancer signal transduction

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