Silencing of the circ-Arhgap5 RNA protects neuronal PC12 cells against injury and depends on the miR-29a-3p/Rock1 axis

Wang, Shengyuan; Li, Na; Meng, Xiang-En

doi:10.1007/s11011-023-01178-7

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…12,27 Overexpression of microRNA-29a-3p can protect neuronal cells from OGD/R injury by downregulating Rock1 or regulating TNFRSF1A and NF-κB signal transduction. 28, 13 29 The expression of microRNA-29a-5p in the plasma of stroke patients significantly decreased after stroke onset. Knocking out microRNA-29a resulted in increased polarization of M1 microglia, leading to an increase in infarct volume in the rat stroke model.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-29a-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation Injury in Astrocytes by Targeting Glycogen Synthase Kinase 3β

Li,

Zhuang,

Liu

et al. 2024

Preprint

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Background: Cerebral ischemia-reperfusion injury (CIRI) after endovascular reperfusion treatment is associated with adverse prognosis in acute ischemic stroke patients. MicroRNAs contribute to CIRI and become the diagnostic and prognosis biomarkers for acute ischemic stroke. In this study, we investigate the role of microRNA-29a-5p in CIRI in the oxygen-glucose deprivation and reoxygenation (OGD/R) model of neurovascular cells. Methods: The expression of microRNA-29a-5p in rat neurons, astrocytes, brain microvascular endothelial cells, microglia, and pericytes model of OGD/R were assessed. The astrocyte injury, phenotype shifting of reactive astrocytes, and regulation of microRNA-29a-5p target genes were evaluated after microRNA-29a-5p mimics and inhibitors treatment in the OGD/R model. Results: MicroRNA-29a-5p decreased in the astrocyte model 24 hours after OGD/R but did not significantly change in the other neurovascular cells after OGD/R. Twelve predicted target genes for microRNA-29a-5p were significantly differentially expressed in the astrocyte OGD/R model; eleven participated in the Wnt signaling pathway. Increased microRNA-29a-5p alleviated astrocyte injury and cell apoptosis. Overexpression of microRNA-29a-5p suppressed neurotoxic A1 astrocyte markers of complement 3, FK506 binding protein 51, and Serping1 and increased neuroprotective A2 astrocyte markers of S100a10, Pentraxin 3, and Emp1. MicroRNA-29a-5p effectively regulated the direct target gene of Glycogen synthase kinase (GSK)-3beta expression and its downstream beta-catenin in astrocytes after OGD/R. Conclusions: MicroRNA-29a-5p alleviated astrocyte injury, transformed the A1/A2 phenotype of reactive astrocyte, and regulated its direct target gene of GSK-3beta and its downstream mediator of beta-catenin in astrocytes after OGD/R. Astrocytic microRNA-29a-5p may be a protective target for reducing CIRI.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-29a-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation Injury in Astrocytes by Targeting Glycogen Synthase Kinase 3β

Li,

Zhuang,

Liu

et al. 2024

Preprint

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“…TargetScan (http://www.targetscan.org) was utilized in this study for the purpose of analyzing and predicting miRNAs that govern DCX mRNA, and analysis results showed miR-29a-3p was one of them. Through relevant literature, it was report miR-29a-3p is involved in various cellular processes [24][25][26][27][28][29] , but its role in NSCs to neuronal differentiation process is unclear. We speculate miR-29a-3p may participate in process of NSCs to neuronal differentiation by targeting DCX.…”

mentioning

confidence: 99%

The Impact of microRNA-29a-3p on the Neural Stem Cell Differentiation Process into Neurons

Shi,

Zhou,

Dai

et al. 2023

IJPS

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Shi et al.: microRNA-29a-3p Impact on the Neural Stem Cell DifferentiationTo explore how microRNA-29a-3p influences the differentiation of neural stem cells into neurons and to identify any potential mechanisms involved. The program TargetScan, available online was utilized to examine and make predictions about the microRNA-29a-3p located upstream that regulate doublecortin X messenger ribonucleic acid. Ultimately, microRNA-29a-3p was preferred as the main central point of the investigation. The targeting of doublecortin X gene by microRNA-29a-3p was validated by employing a dual-luciferase reporter gene system. Before subjecting to adherent differentiation, the neural stem cells were transfected with microRNA-29a-3p inhibitor, microRNA-29a-3p mimic and normal control. MicroRNA and doublecortin X messenger ribonucleic acid levels were measured through quantitative reverse transcriptionpolymerase chain reaction and doublecortin X protein levels were determined through Western blot assays. The differentiation of neuronal precursor cells was detected by doublecortin X immunofluorescence. Neuronal differentiation was detected by microtubule-associated protein 2 immunofluorescence. According to the dual-luciferase reporter gene system, miR-29a-3p targeted the doublecortin X gene; microRNA-29a-3p significantly decreased in differentiated cells for 3 d was observed, along with a significant reduction in levels of doublecortin X protein and messenger ribonucleic acid. On the other hand, a significant raise in microRNA-29a-3p in differentiated cells of microRNA-29a-3p mimic group for a period of 3 d was noticed with a significant drop in protein levels, doublecortin X messenger ribonucleic acid and doublecortin X immunofluorescence-positive neuronal precursor cells. From the microtubule-associated protein 2 immunofluorescence, it was confirmed that microRNA-29a-3p inhibitor group significantly elevated the total number of neurons, while microRNA-29a-3p mimic reduced the neurons on 7 d. Therefore, low expression of microRNA-29a-3p promote differentiation of neural stem cells into neurons via targeting doublecortin X.

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CircRNA: A new target for ischemic stroke

Yifan,

Jiaheng,

Yili

et al. 2025

Gene

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Silencing of the circ-Arhgap5 RNA protects neuronal PC12 cells against injury and depends on the miR-29a-3p/Rock1 axis

Cited by 3 publications

References 34 publications

MicroRNA-29a-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation Injury in Astrocytes by Targeting Glycogen Synthase Kinase 3β

MicroRNA-29a-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation Injury in Astrocytes by Targeting Glycogen Synthase Kinase 3β

The Impact of microRNA-29a-3p on the Neural Stem Cell Differentiation Process into Neurons

CircRNA: A new target for ischemic stroke

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