Silencing of RB1 and RB2/P130 during adipogenesis of bone marrow stromal cells results in dysregulated differentiation

Capasso, Stefania; Alessio, Nicola; Bernardo, Giovanni Di; Cipollaro, Marilena; Melone, Mariarosa Anna Beatrice; Peluso, Gianfranco; Giordano, Antonio; Galderisi, Umberto

doi:10.4161/cc.27275

Cited by 20 publications

(16 citation statements)

References 36 publications

(50 reference statements)

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“…Bone marrow adipocyte lineage cells were proved to regulate bone marrow stem cell niche [17]; these fat-storing cells secrete a protein hormone named 'Leptin' that regulates food intake as well as some metabolic and endocrine functions. Bone marrow adipocyte lineage cells were proved to regulate bone marrow stem cell niche [17]; these fat-storing cells secrete a protein hormone named 'Leptin' that regulates food intake as well as some metabolic and endocrine functions.…”

Section: Resultsmentioning

confidence: 99%

Histological study of megakaryocytogenesis and thrombocytogenesis in the guinea pig bone marrow with reference to the role of adipocytes

El-Bassouny

2014

The Egyptian Journal of Histology

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Section: Resultsmentioning

confidence: 99%

Histological study of megakaryocytogenesis and thrombocytogenesis in the guinea pig bone marrow with reference to the role of adipocytes

El-Bassouny

2014

The Egyptian Journal of Histology

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“…In 2008, the first murine miRNA, miR-17-92, was being elucidated to promote adipocyte differentiation [45]. Interestingly, with its validated direct target Rb2/p130, known to be involved in cell cycle regulation, miR-17-92 has an impact on the balance between proliferation and differentiation towards the latter [46,47]. Subsequently, further pro-adipogenic miRNAs have been elucidated, such as miR-204 and miR-211 both able to repress Runx2 [48], miR-210 repressing anti-adipogenic Wnt signaling through targeting Tcf7l2 and activating the Pi3k/Akt pathway via targeting Ship1 [49,50], miR-103 activating Akt/mTor signaling by direct targeting of the anti-adipogenic Mef2d [51], and miR-125b for which a direct target that mediates the pro-adipogenic miRNA effect has not yet been identified [52].…”

Section: Mirnas In Murine White Adipogenesismentioning

confidence: 99%

MicroRNAs in adipocyte formation and obesity

Scheideler

2016

Best Practice & Research Clinical Endocrinology & Metab

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The worldwide epidemic of obesity demands novel and more effective therapeutic approaches. Fat cells are at the core of energy metabolism trying either to cope with a positive energy balance by hypertrophy and hyperplasia of energy storing white adipocytes or to counteract obesity by the induction of non-shivering thermogenesis in energy combusting brite/brown adipocytes. However, the comprehensive regulatory network of adipocyte formation remains to be elucidated. MicroRNAs are an emerging class of important regulatory determinants in many biological processes and diseases, including adipocyte formation and obesity. In this review, microRNAs governing the formation of white, brite and brown adipocytes as well as candidates with impact on obesity are overviewed, concluded with recommendations for further research that considers prerequisites for successful therapeutic applications.

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“…The classic role for the retinoblastoma family genes RB1, RB2/P130, and P107 is the regulation of the cell cycle G 1 /S transition through the negative modulation of the E2F family of transcription factors. Data demonstrated that lack of RB1 and RB2/P130 increased the percentage of BM‐MSC committed toward adipocyte phenotype (Capasso et al, ). In particular, the adipocytes appeared not to reach a terminal differentiation, or alternatively, showed dysregulated functions.…”

Section: Introductionmentioning

confidence: 99%

“…MAs do not proliferate but the growth of adipose tissue occurs through the formation of new adipocytes or by increasing the volume of adipocytes (Rosen & Spiegelman, ). Even if some aspects of in vitro adipocytes differentiation are controversial, it is now clear that some cell cycle checkpoint proteins, such as the retinoblastoma gene family, influence adipogenesis (Capasso et al, ). The classic role for the retinoblastoma family genes RB1, RB2/P130, and P107 is the regulation of the cell cycle G 1 /S transition through the negative modulation of the E2F family of transcription factors.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, in adipocytes lacking RB1 or RB2, the mean cellular level of early differentiation markers persisted at a high level. Moreover, adipocytes lacking RB2 showed a higher propensity in lipid uptake compared with control cells, while adipocytes lacking RB1 showed a higher capacity in lipid release (Capasso et al, ). All together results showed that commitment of BM‐MSC to adipocyte lineage was facilitated by a lack of RB1 and RB2.…”

Section: Introductionmentioning

confidence: 99%

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Key‐genes regulating the liposecretion process of mature adipocytes

Maurizi

Petäistö

Maurizi³

et al. 2017

Journal Cellular Physiology

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White mature adipocytes (MAs) are plastic cells able to reversibly transdifferentiate toward fibroblast-like cells maintaining stem cell gene signatures. The main morphologic aspect of this transdifferentiation process, called liposecretion, is the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion. There is a considerable interest in the adipocyte plastic properties involving liposecretion process, but the molecular details are incompletely explored. This review analyzes the gene expression of MAs isolated from human subcutaneous fat tissue with respect to bone marrow (BM)-derived mesenchymal stem cells (MSC) focusing on gene regulatory pathways involved into cellular morphology changes, cellular proliferation and transports of molecules through the membrane, suggesting potential ways to guide liposecretion. In particular, Wnt, MAPK/ERK, and AKT pathways were accurately described, studying up- and down-stream molecules involved. Moreover, adipogenic extra- and intra-cellular interactions were analyzed studying the role of CDH2, CDH11, ITGA5, E-Syt1, PAI-1, IGF1, and INHBB genes. Additionally, PLIN1 and PLIN2 could be key-genes of liposecretion process regulating molecules transport through the membrane. All together data demonstrated that liposecretion is regulated through a complex molecular networks that are able to respond to microenvironment signals, cytokines, and growth factors. Autocrine as well as external signaling molecules might activate liposecretion affecting adipocytes physiology.

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Silencing of RB1 and RB2/P130 during adipogenesis of bone marrow stromal cells results in dysregulated differentiation

Cited by 20 publications

References 36 publications

Histological study of megakaryocytogenesis and thrombocytogenesis in the guinea pig bone marrow with reference to the role of adipocytes

Histological study of megakaryocytogenesis and thrombocytogenesis in the guinea pig bone marrow with reference to the role of adipocytes

MicroRNAs in adipocyte formation and obesity

Key‐genes regulating the liposecretion process of mature adipocytes

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