Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants

Yang, Kiwoung; Kim, Hee Seung; Jin, Un Ho; Lee, Sang Sook; Park, Jong A.; Lim, Yong Pyo; Pai, Hyun Sook

doi:10.1007/s00425-006-0453-3

Cited by 44 publications

(28 citation statements)

References 44 publications

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“…These results are similar to some other studies demonstrating that BTF3 take part in cell cycle regulation via interaction with cyclins [25]. Mutants lacking NAC also showed visible growth and morphological problem in yeast and tobacco [20, 26]. Furthermore, the inhibition of BTF3 participated in the repression of transcription and protein synthesis in apoptotic K562 cells [27], while change in BTF3 expression is linked with apoptosis in BL60 Burkitt lymphoma cells [28].…”

Section: Discussionsupporting

confidence: 87%

“…In Osj10gBTF3 Ri seedling, the expression level significantly decreased in root and shoot with increasing ABA concentration (Figure 6). Wang et al [6] observed a 62% increase in Osj10gBTF3 expression level with ABA, compared to a 22% reduction with GA 3 after treatment with the same concentration for 12 h. In previous reports, it has been observed that the transcript level of NbBTF3 remained unaffected when exposed to 100 μ M of ABA and GA 3 [26]. Similarly, He et al [29] did not find any significant change in SmBTF3, when treated with ABA for 24 hrs.…”

Section: Discussionmentioning

confidence: 96%

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Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

Wang

et al. 2014

BioMed Research International

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BTF3 has been recognized to be involved in plant growth and development. But its function remains mostly unknown during seed germination and seedling stage. Here, we have analyzed OsBTF3-related sequences in Oryza sativa L. subspecies, japonica, which resembles with the conserved domain of a nascent polypeptide associated complex (NAC) with different homologs of OsBTF3 and human BTF3. Inhibition of Osj10gBTF3 has led to considerable morphological changes during seed germination and seedling growth. Germination percentage was not influenced by the application of GA3, ABA, and NaCl but all concentrations caused wild-type (WT) seeds to germinate more rapidly than the RNAi (Osj10gBTF3 Ri) transgenic lines. Seedling inhibition was more severe in the Osj10gBTF3 Ri seedlings compared with their WT especially when treated with 100 or 200 μM GA3; 50% reduction in shoots was observed in Osj10gBTF3 Ri seedlings. The expression of Osj3g1BTF3, Osj3g2BTF3 and Osj10gBTF3 was primarily constitutive and generally modulated by NaCl, ABA, and GA3 stresses in both Osj10gBTF3 Ri lines and WT at the early seedling stage, suggesting that Osj3g1BTF3 and Osj10gBTF3 are much similar but different from Osj3g2BTF3 in biological function. These results show that OsBTF3 plays an important role in seed germination and seedling growth gives a new perception demonstrating that more multifaceted regulatory functions are linked with BTF3 in plants.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 96%

Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

Wang

et al. 2014

BioMed Research International

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“…The sugarcane Nap is homologous to bNAP or BTF3. Silencing of NbBTF3 (bNAP) expression in Nicotiana benthamiana reduced chloroplast sizes and chlorophyll contents and the cells produced excessive amounts of ROS (Yang et al 2007). Sugarcane NAP could be regarded as a factor controlling homeostasis in chloroplasts, mitochondria and possibly the endoplasmic reticulum.…”

Section: Rust Infection-induced Metabolic Changesmentioning

confidence: 99%

Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling

et al. 2012

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Brown rust caused by the fungus Puccinia melanocephala is a major disease of sugarcane (Saccharum spp.). A sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety B4362, showed a post-haustorial hypersensitive response (HR)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to P. melanocephala via suppression subtractive hybridization (SSH). Tester cDNA was derived from the brown rust-resistant mutant after inoculation with P. melanocephala, while driver cDNAs were obtained from the non-inoculated resistant mutant and the inoculated susceptible donor variety B4362. Database comparisons of the sequences of the SSH recombinant clones revealed that, of a subset of 89 non-redundant sequences, 88% had similarity to known functional genes, while 12% were of unknown function. Thirteen genes were selected for transcript profiling in the resistant mutant and the susceptible donor variety. Genes involved in glycolysis and C4 carbon fixation were up-regulated in both interactions probably due to disturbance of sugarcane carbon metabolism by the pathogen. Genes related with the nascent polypeptide associated complex, post-translational proteome modulation and autophagy were transcribed at higher levels in the compatible interaction. Up-regulation of a putative L-isoaspartyl O-methyltransferase S-adenosylmethionine gene in the compatible interaction may point to fungal manipulation of the cytoplasmatic methionine cycle. Genes coding for a putative no apical meristem protein, S-adenosylmethionine decarboxylase, non-specific lipid transfer protein, and GDP-L-galactose phosphorylase involved in ascorbic acid biosynthesis were up-regulated in the incompatible interaction at the onset of haustorium formation, and may contribute to the HR-mediated defense response in the rust-resistant mutant.

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“…In higher plants, transcription factor BTF3 is required for RNA polymerase II-dependent transcription. The downregulation of BTF3 expression reduces the chloroplast size and the chlorophyll content [46]. Therefore, the upregulation of BTF3 activity may contribute to chloroplast development in Chlorella to accommodate light stress.…”

Section: Discussionmentioning

confidence: 99%

Suppression Subtractive Hybridization Reveals Transcript Profiling of Chlorella under Heterotrophy to Photoautotrophy Transition

et al. 2012

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BackgroundMicroalgae have been extensively investigated and exploited because of their competitive nutritive bioproducts and biofuel production ability. Chlorella are green algae that can grow well heterotrophically and photoautotrophically. Previous studies proved that shifting from heterotrophy to photoautotrophy in light-induced environments causes photooxidative damage as well as distinct physiologic features that lead to dynamic changes in Chlorella intracellular components, which have great potential in algal health food and biofuel production. However, the molecular mechanisms underlying the trophic transition remain unclear.Methodology/Principal FindingsIn this study, suppression subtractive hybridization strategy was employed to screen and characterize genes that are differentially expressed in response to the light-induced shift from heterotrophy to photoautotrophy. Expressed sequence tags (ESTs) were obtained from 770 and 803 randomly selected clones among the forward and reverse libraries, respectively. Sequence analysis identified 544 unique genes in the two libraries. The functional annotation of the assembled unigenes demonstrated that 164 (63.1%) from the forward library and 62 (21.8%) from the reverse showed significant similarities with the sequences in the NCBI non-redundant database. The time-course expression patterns of 38 selected differentially expressed genes further confirmed their responsiveness to a diverse trophic status. The majority of the genes enriched in the subtracted libraries were associated with energy metabolism, amino acid metabolism, protein synthesis, carbohydrate metabolism, and stress defense.Conclusions/SignificanceThe data presented here offer the first insights into the molecular foundation underlying the diverse microalgal trophic niche. In addition, the results can be used as a reference for unraveling candidate genes associated with the transition of Chlorella from heterotrophy to photoautotrophy, which holds great potential for further improving its lipid and nutrient production.

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Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants

Cited by 44 publications

References 44 publications

Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling

Suppression Subtractive Hybridization Reveals Transcript Profiling of Chlorella under Heterotrophy to Photoautotrophy Transition

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