Silencing of IQGAP1 by shRNA inhibits the invasion of ovarian carcinoma HO-8910PM cells in vitro

Abstract

Background: IQGAP1 is a scaffolding protein and overexpressed in many human tumors, including ovarian cancer. However, the contribution of IQGAP1 to invasive properties of ovarian cancer cells remains unknown. Here, we investigated the effect of IQGAP1-specific short hairpin RNA (shRNA) expressing plasmids on metastatic potential of ovarian cancer HO-8910PM cells.

Chlamydophila (Chlamydia) pneumoniae infection promotes vascular smooth muscle cell adhesion and migration through IQ domain GTPase-activating protein 1

“…Bogatkevich et al [43] found that silencing the expression of IQGAP1 by siRNA inhibited connective tissue growth factor-induced lung fibroblast migration. RNAimediated knockdown of IQGAP1 expression in human ovarian cancer HO-8910PM cells resulted in a significant decrease in cell invasion and migration [44]. The data presented in this study as well as in the above reports all reveal a promotive role of IQGAP1 in the control of cell migration.…”

Differential Expression of IQGAP1/2 in Hepatocellular Carcinoma and its Relationship with Clinical Outcomes

“…IQGAP1 is overexpressed in colorectal carcinoma (Nabeshima et al, 2002), breast cancer (Jadeski et al, 2008), aggressive ovarian adenocarcinomas (Dong et al, 2008) and gastric cancer (Walch et al, 2008), while IQGAP2 expression is lost in gastric carcinoma (Jin et al, 2008), prostate (Xie et al, 2012) and liver cancer (Schmidt, 2012;Gnatenko et al, 2013). It was also reported that IQGAP1 expression is up-regulated in HCC, characterized by the loss of membrane E-cadherin expression, the cytoplasmic translocation of β-catenin, and the overexpression of nuclear target of β-catenin, cyclin D1 (Chen et al, 2010).…”

MicroRNA-101 targets EZH2, MCL-1 and FOS to suppress proliferation, invasion and stem cell-like phenotype of aggressive endometrial cancer cells

“…Invasion assays were performed were performed using Transwell system (8-μm pore size, matrigel-coated polycarbonate membrane BD Biosciences, Bedford, MA), as described previously [57]. Briefly, 5 × 10 4 cells resuspended in serum-free medium was added to the upper inserts.…”