Silencing of Glutamine: Fructose-6-Phosphate Aminotransferase Impairs Growth and Development in Sogatella furcifera (Hemiptera: Delphacidae)

Wang, Zhao; Long, Guiyun; Zhu, Huan; Jin, Daochao; Yang, Hong; Zhou, Cao

doi:10.3390/biom13101433

Cited by 2 publications

References 46 publications

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Conserved microRNAs miR-8-3p and miR-2a-3 targeting chitin biosynthesis to regulate the molting process of Sogatella furcifera (Horváth)(Hemiptera: Delphacidae)

Ren,

Long,

Yang

et al. 2024

Journal of Economic Entomology

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Molting is a key solution to growth restriction in insects. The periodic synthesis and degradation of chitin, one of the major components of the insect epidermis, is necessary for insect growth. MicroRNA (miRNA) have been implicated in molting regulation, yet their involvement in the interplay interaction between the chitin synthesis pathway and 20-hydroxyecdysone signaling remains poorly understood. In this study, soluble trehalase (Tre1) and phosphoacetylglucosamine mutase (PAGM) were identified as targets of conserved miR-8-3p and miR-2a-3, respectively. The expression profiles of miR-8-3p-SfTre1 and miR-2a-3-SfPAGM exhibited an opposite pattern during the different developmental stages, indicating a negative regulatory relationship between them. This relationship was confirmed by an in vitro dual-luciferase reporter system. Overexpression of miR-8-3p and miR-2a-3 by injection of mimics inhibited the expression of their respective target genes and increased mortality, leading to death in the pre-molting, and molting death phenomena. They also caused a decrease in chitin content and expression levels of key genes in the chitin synthesis pathway (SfTre1, SfTre2, SfHK, SfG6PI, SfGFAT, SfGNA, SfPAGM, SfUAP, SfCHS1, SfCHS1a, and SfCHS1b). Conversely, the injection of miRNA inhibitors resulted in the upregulation of the expression levels of these genes. Following 20E treatment, the expression levels of miR-8-3p and miR-2a-3 decreased significantly, while their corresponding target genes increased significantly. These results indicate that miR-8-3p and miR-2a-3 play a regulatory role in the molting of Sogatella furcifera by targeting SfTre1 and SfPAGM, respectively. These findings provide new potential targets for the development of subsequent new control strategies.

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Conserved microRNAs miR-8-3p and miR-2a-3 targeting chitin biosynthesis to regulate the molting process of Sogatella furcifera (Horváth)(Hemiptera: Delphacidae)

Ren,

Long,

Yang

et al. 2024

Journal of Economic Entomology

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Intestinimonas butyriciproducens affects Eimeria tenella development and infection via regulating EtGFAT gene expression

Li,

Huang,

Shi

et al. 2024

Preprint

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Chicken coccidiosis, caused by Eimeria, is a prevalent problem worldwide and results in significant economic losses to the poultry industry. The chicken cecum harbors a diverse and complex microbiota, with E. tenella exclusively parasitizing the cecum and thus engaging in complex interactions with the cecal microbiota. In the investigation, an antibiotic-induced cecal microbiota depletion model was used to study the relationship between E. tenella and the microbial community for the first time. It was revealed that the absence of cecal microbiota can disrupt E. tenella macrogametes development. Furthermore, the critical role of cecal microbiota integrity in E. tenella development was confirmed through fecal microbiota transplantation experiments. Notably, the study identified the genus Intestinimonas within the cecum as a primary factor influencing E. tenella development. Specifically, it was discovered that the genus Intestinimonas significantly affects macrogametes development through EtGFAT gene expression. In addition, transplantation of Intestinimonas butyriciproducens resulted in a significant reduction in clinical symptoms associated with E. tenella infection. Furthermore, Intestinimonas butyriciproducens promoted the secretion of high levels of IFN-γ from CD8 + T cells, which in turn exerts resistance to E. tenella. These findings offer valuable insights for developing innovative anti-coccidia agents.

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Silencing of Glutamine: Fructose-6-Phosphate Aminotransferase Impairs Growth and Development in Sogatella furcifera (Hemiptera: Delphacidae)

Cited by 2 publications

References 46 publications

Conserved microRNAs miR-8-3p and miR-2a-3 targeting chitin biosynthesis to regulate the molting process of Sogatella furcifera (Horváth)(Hemiptera: Delphacidae)

Conserved microRNAs miR-8-3p and miR-2a-3 targeting chitin biosynthesis to regulate the molting process of Sogatella furcifera (Horváth)(Hemiptera: Delphacidae)

Intestinimonas butyriciproducens affects Eimeria tenella development and infection via regulating EtGFAT gene expression

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