Silencing of FOS-like antigen 1 represses restenosis <i>via</i> the ERK/AP-1 pathway in type 2 diabetic mice

Zhou, Chenguang; Wang, Fujun; Hanson, Mark A.; Xing, Na; Hou, Lin; Du, Yaping; Ding, Hu

doi:10.1177/14791641211058855

Cited by 7 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research has shown that LMNA (lamin A/C) [314], VCAM1 [281], MAPK6 [403], DDX3X [366], SHMT2 [459], PHGDH (phosphoglycerate dehydrogenase) [560], S100A9 [433], SNCA (synuclein alpha) [335], TP63 [313], FOSL2 [1449], FHL2 [321], ATF3 [55], FOSL1 [379], DYSF (dysferlin) [475], CA4 [547], S100A8 [58] and MPO (myeloperoxidase) [521] plays an important role in the pathogenesis of lung cancer. LMNA (lamin A/C) [676], VCAM1 [657], DDX3X [708], S100A9 [745], FKBP5 [747], SNCA (synuclein alpha) [690], FOSL2 [1450], FHL2 [680], ATF3 [63], FOSL1 [718], S100A8 [67] and MPO (myeloperoxidase) [787] altered expression is associated with poor prognosis of diabetes mellitus. LMNA (lamin A/C) [676], VCAM1 [986], PHGDH (phosphoglycerate dehydrogenase) [1100], S100A9 [1045], FKBP5 [1046], FHL2 [1003], ATF3 [85] and MPO (myeloperoxidase) [1084] plays an important role in the development of obesity.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes and biological pathways in chronic obstructive pulmonary disease using bioinformatics and next generation sequencing data analysis

Vastrad,

Vastrad

2024

Preprint

View full text Add to dashboard Cite

Identification of accurate biomarkers is still particularly urgent for improving the poor survival of chronic obstructive pulmonary disease (COPD) patients. In this investigation, we aimed to identity the potential biomarkers in COPD via bioinformatics and next generation sequencing (NGS) data analysis. In this investigation, the differentially expressed genes (DEGs) in COPD were identified using NGS dataset (GSE239897) from Gene Expression Omnibus (GEO) database. Subsequently, gene ontology (GO) and pathway enrichment analysis was conducted to evaluate the underlying molecular mechanisms involved in progression of COPD. Protein-protein interaction (PPI), modules, miRNA-hub gene regulatory network and TF-hub gene regulatory network analysis were performed to determine the hub genes, miRNAs and TFs. The receiver operating characteristic (ROC) analysis was performed to determine the diagnostic value of hub genes. A total of 956 overlapping DEGs (478 up regulated and 478 down regulated genes) were identified in the NGS dataset. DEGs were mainly associated with GO functional terms and pathways in cellular response to stimulus. response to stimulus, immune system and neutrophil degranulation. There were 10 hub genes (MYC, LMNA, VCAM1, MAPK6, DDX3X, SHMT2, PHGDH, S100A9, FKBP5 and RPS6KA2) identified by PPI, modules, miRNA-hub gene regulatory network and TF-hub gene regulatory network analysis. In conclusion, the DEGs, relative GO terms, pathways and hub genes identified in the present investigation might aid in understanding of the molecular mechanisms underlying COPD progression and provide potential molecular targets and biomarkers for COPD.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of key genes and biological pathways in chronic obstructive pulmonary disease using bioinformatics and next generation sequencing data analysis

Vastrad,

Vastrad

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition to involve in the Wnt signaling pathway, FOSL1 can inhibit adipocyte differentiation by inhibiting C/EBPa (46). FOSL1 is a potential therapeutic target to prevent diabetes-induced atherosclerosis (47). The existing literatures showed that lncRNAs, such as TCONS_00006525, TCONS_00046551, and TCONS_00000528 in the regulatory network are involved in the WNT signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs

et al. 2023

View full text Add to dashboard Cite

BackgroundLong non-coding RNAs (lncRNAs) regulate adipocyte differentiation and metabolism, However, their function on subcutaneous and intramuscular adipose tissues in pigs is unclear. Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), which severely affects the meat rate of pigs. It is of great significance for porcine breeding to study the mechanism of lncRNA related to adipogenesis and lipid metabolism.MethodsWe identified differentially expressed lncRNAs, miRNAs and mRNAs in subcutaneous and intramuscular adipose tissues in three female Laiwu pigs by deep RNA-sequencing(|log2foldchange|≥1, P_value ≤ 0.05). The gene expression profiles of IMF and SCF in Laiwu pigs were comparatively analyzed by Bioinformatics methods to identify key lncRNAs, miRNAs, and mRNAs associated with lipid metabolism and adipogenesis.ResultsA total of 1209 lncRNAs (DElncRNAs), 286 miRNAs (DEmiRNAs), and 1597 mRNAs (DEgenes) were differentially expressed between two types of adipose. Among them, 17 DElncRNAs and 103 target genes play a role in the co-expression network, as well as 59 DElncRNAs, 44 DEmiRNAs, and 88 DEgenes involved in ceRNA network. In GO(Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of DElncRNAs their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as PPAR signaling pathway, Wnt signaling pathway, MAPK signaling pathway.ConclusionsBy constructing co-expression and ceRNAs network we found that Wnt signaling pathway play a critical regulatory role in intramuscular adipogenesis and lipid accumulation in Laiwu pigs. TCONS_00006525, TCONS_00046551 and TCONS_00000528 may target WNT5A, WNT10B and FDZ3 in co-expression network, TCONS_00026517 and other lncRNAs regulate the expression of PPARG, RXRG and SCD in ceRNA network, and were involved in Wnt signaling pathway. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation, predicting and treating diseases caused by ectopic fat.

show abstract

“…Although there is evidence that targeted interventions for diabetic foot ulcers and multidisciplinary care can reduce the loss of limbs, the progress has been slow so far [22][23][24]. Although the elderly population with glycosuria amputation is declining, the number of patients with type 2 diabetes has increased in some countries [25,26]. In this experiment, the diabetic mouse foot ulcer model was established to observe the curative effect of external ulcer powder on mouse foot ulcers and the expression of angiogenesis-related factors ERK and VEGF.…”

Section: Discussionmentioning

confidence: 99%

The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice

Wang¹,

Xie²,

Lu³

et al. 2022

Cureus

View full text Add to dashboard Cite

Through the preparation of the diabetic mice skin ulcer model, we investigated the effect of Mongolian medicine external ulcer powder (WYK) on the treatment of diabetic skin ulcers and the expression of angiogenesis-related factors such as vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK). MethodsThirty male clean Kunming mice were randomly divided into normal control group (group C), diabetic control group (group HC), and diabetic topical ulcer powder group (group HW). After successful modeling in the HC group and the HW group, the rats in the HW group were given external ulcer powder, which was applied to the back of the mice once a day. In addition, the rats in group C and group HC were treated with gentamicin injection external application once a day. The mice were sacrificed on the 3rd, the sixth, and the ninth day of dosing, and samples were taken. The adopted methods included protein immunoblotting (western blot) and reverse transcription-polymerase chain reaction (RT-PCR). The expression differences of angiogenesis-related factors such as VEGF and ERK in the repair process were detected. SPSS 13 software was used to analyze the results of angiogenesis-related factors VEGF and ERK. Results Comparison of VEGF and ERK ContentsThe serum VEGF content of mice in the HC group was significantly lower than that in the C group on days 3, 6, and 9 (p <0.05). The VEGF content in the HW group was significantly higher than that in the HC group (p <0.05). The content of ERK in serum was basically consistent with that of VEGF. The results of the western blot assay were consistent with those of the RT-PCR assay. ConclusionWYK can effectively promote the healing of skin ulcer wounds in diabetic mice, accelerate the proliferation of granulation tissue, enrich the contents of capillary blood tubes and collagen fibers, and increase the microvascular content. WYK can improve the expression level of VEGF and ERK in the serum of mice and advance the peak value of protein expression.

show abstract

Silencing of FOS-like antigen 1 represses restenosis via the ERK/AP-1 pathway in type 2 diabetic mice

Cited by 7 publications

References 20 publications

Identification of key genes and biological pathways in chronic obstructive pulmonary disease using bioinformatics and next generation sequencing data analysis

Identification of key genes and biological pathways in chronic obstructive pulmonary disease using bioinformatics and next generation sequencing data analysis

Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs

The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice

Contact Info

Product

Resources

About