Silencing of acetylcholinesterase gene of Helicoverpa armigera by siRNA affects larval growth and its life cycle

Validation of reference genes in leaf-cutting ant Atta sexdens rubropilosa in different developmental stages and tissues

“…5a). AChE from Anopheles gambiae also showed a similar expression pattern and some works have shown that this enzyme also exhibit a noncholinergic functions associated with insect development [43,44,45]. In tissues, there is a small variation in the expression of AChE in the head compared to the mesosoma, while in worker without gaster, that is the junction of the other two, the expression is almost 2-fold higher than in mesosoma (Fig.…”

Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

“…When treatment of 1.36 µg acetylcholinesterase siRNA was withdrawn after feeding up to 2 nd -instar, larvae attained normal growth, with comparable pupal weight to controls and successfully completed their life cycle. Larvae subjected to continuous feeding on the same amount of siRNA, however, exhibited 81% weight reduction, arrested growth and ultimately did not reach maturity (Kumar et al, 2009). …”

Insect Pest Management in Conservation Agriculture

“…The suppression of gene expression by specific dsRNAs was extended to herbivorous insects such as herbivorous lepidopteran larvae, Epiphyas postvittana, fed with dsRNAs in solution by droplet feeding (Turner et al 2006). Kumar et al (2009) demonstrated that a diet incorporating feeding of synthesized small interfering RNA (siRNA) to larvae of corn earworm ( H. armigera) resulted in specific suppression of expression of an acetylcholinesterase gene with effects observed at mRNA and protein levels. Feeding dsRNA to larvae of diamondback moth ( Plutella xylostella) also produced RNAi-mediated gene suppression (Bautista and Miyata 2009;Kumar et al 2009).…”