Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses

Seng, Shanshan; Wu, Chenyu; Wu, Jian; Zhong, X.P.; He, Junna; Yi, Mingfang

doi:10.1007/s11240-019-01711-6

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…CATALASE2 (CAT2) promotes JA-biosynthetic acyl-CoA oxidase (ACX) activity to enhance plant resistance to the necrotrophic Botrytis cinerea [189]. In addition, prior exposure of Arabidopsis seedlings to mechanical stress enhanced JA-mediated defense against necrotrophic pathogens [190], and GhCOI1 is an essential signaling component that controls the JA-regulated defense response against Alternaria brassicicola in Gladiolus plants [191].…”

Section: Plant Disease Resistancementioning

confidence: 99%

Function and Mechanism of Jasmonic Acid in Plant Responses to Abiotic and Biotic Stresses

Wang

Mostafa

Zeng

et al. 2021

IJMS

238

108

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As sessile organisms, plants must tolerate various environmental stresses. Plant hormones play vital roles in plant responses to biotic and abiotic stresses. Among these hormones, jasmonic acid (JA) and its precursors and derivatives (jasmonates, JAs) play important roles in the mediation of plant responses and defenses to biotic and abiotic stresses and have received extensive research attention. Although some reviews of JAs are available, this review focuses on JAs in the regulation of plant stress responses, as well as JA synthesis, metabolism, and signaling pathways. We summarize recent progress in clarifying the functions and mechanisms of JAs in plant responses to abiotic stresses (drought, cold, salt, heat, and heavy metal toxicity) and biotic stresses (pathogen, insect, and herbivore). Meanwhile, the crosstalk of JA with various other plant hormones regulates the balance between plant growth and defense. Therefore, we review the crosstalk of JAs with other phytohormones, including auxin, gibberellic acid, salicylic acid, brassinosteroid, ethylene, and abscisic acid. Finally, we discuss current issues and future opportunities in research into JAs in plant stress responses.

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Section: Plant Disease Resistancementioning

confidence: 99%

Function and Mechanism of Jasmonic Acid in Plant Responses to Abiotic and Biotic Stresses

Wang

Mostafa

Zeng

et al. 2021

IJMS

238

108

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“…In this experiment, we have tried various methods for silencing peony PlWRKY13 , and finally adopted the vacuum suction filtration under negative pressure method with the highest silencing efficiency. As a convenient technique to explore gene function widely used in recent years, VIGS is more intuitive in phenotypic variation, especially applicable to species with unstable genetic transformation system, such as grapevine [47], citrus [48], tomato [49], gladiolus [50], etc. However, the use of VIGS still has some disadvantages, including the limited duration of silence, different viral vectors will produce different silence efficiency for different species [48].…”

Section: Discussionmentioning

confidence: 99%

PlWRKY13: A Transcription Factor Involved in Abiotic and Biotic Stress Responses in Paeonia lactiflora

Wang

Guo

et al. 2019

IJMS

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Many members of the WRKY family regulate plant growth and development. Recent studies have shown that members of the WRKY family, specifically WRKY13, play various roles in the regulation of plant stress resistance. To study the function of WRKY family members in peony, the PlWRKY13 gene (KY271095) was cloned from peony leaves. Sequence analysis and subcellular localization results revealed that PlWRKY13 has no introns, belongs to the type IIc subgroup of the WRKY family, and functions in the nucleus. The expression pattern of PlWRKY13 was analysed via real-time quantitative RT-PCR (qRT-PCR), which showed that the expression of PlWRKY13 was induced by four types of abiotic stress, low-temperature, high-temperature, waterlogging and salt stress, and was positively upregulated in response to these stresses. In addition, the expression of PlWRKY13 tended to first decrease and then increase after infection with Alternaria tenuissima. Virus-induced gene silencing (VIGS) technology was used to explore the function of PlWRKY13 in the resistance of Paeonia lactiflora to fungal infection further, and the results showed that PlWRKY13-silenced plants displayed increased sensitivity to A. tenuissima. The infection was more severe and the disease index (DI) significantly greater in the PlWRKY13-silenced plants than in the control plants, and the expression of pathogenesis-related (PR) genes was also significantly altered in the PlWRKY13-silenced plants compared with the control plants. The contents of the endogenous hormones jasmonic acid (JA) and salicylic acid (SA) were measured, and the results showed that the JA content increased gradually after infection with A. tenuissima and that JA may play an active role in the resistance of P. lactiflora to pathogen infection, while the SA content decreased after PlWRKY13 silencing. The contents of the two hormones decreased overall, suggesting that they are related to the transcription of PlWRKY13 and that PlWRKY13 may be involved in the disease-resistance pathway mediated by JA and SA. In summary, the results of our study showed that PlWRKY13 expression was induced by stress and had a positive effect on the resistance of P. lactiflora to fungal infection.

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“…It has been reported that COI1 is not only involved in plant developmental processes, such as leaf senescence [ 18 , 19 ], seed maturation [ 13 ], flowering [ 20 ], male fertility [ 21 ], anthocyanin formation [ 22 ], and root growth [ 22 , 23 ], but also plays a part during various physiological processes in the plant defense response to insect attack and pathogen infection [ 13 , 24 , 25 ]. COI1 belongs to a multigene family.…”

Section: Introductionmentioning

confidence: 99%

“…However, different mutations in the COI1 gene have distinct effects on COI1 function in regulating male fertility. GhCOI1 silencing in Gladiolus hybridus impaired inducible defense and increased susceptibility to the necrotrophic pathogenic fungus Alternaria brassicicola [ 24 ]. The above findings have provided evidence of the importance of the COI1 gene during the processes of plant growth and development, as well as defense responses.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the coronatine-insensitive 1 (COI1) gene family in response to biotic and abiotic stresses in Saccharum

et al. 2022

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Background The coronatine insensitive 1 (COI1) gene is the core member of jasmonate signaling pathway, which is closely related to plant biotic and abiotic resistance. However, there have been no reports on COI1 in sugarcane (Sacharum spp.). Hence, systematically investigating the characteristics of the COI1 multigene family in sugarcane can provide a means to study and manipulate the jasmonic acid signaling pathway. Results A total of 156 COI1 proteins were obtained from the genomes of 19 land plants, while none were obtained from five algae species. A phylogenetic tree demonstrated that these COI1 proteins were classified into four groups, while 31 proteins of SsCOI1 from Saccharum spontaneum, SbCOI1 from Sorghum bicolor, and ShCOI1 from Saccharum spp. hybrid cultivar R570 clustered into three groups. Synteny analysis and duplication patterns revealed that COI1 genes expanded through various genome replication events and could have experienced strong purifying selective pressure during evolution in S. spontaneum, S. bicolor, and R570. An investigation of cis-acting elements suggests that COI1 genes may be involved in plant growth and development and response to various stresses. Expression analysis implied that 21 SsCOI1 genes were constitutively expressed, and had positive responses to drought, cold, and Sporisorium scitamineum stresses with different expression patterns. Among them, seven SsCOI1 haplotype genes may play different roles in response to methyl jasmonate. Furthermore, the ShCOI1–4, ShCOI1–5, and ShCOI1–6 genes were cloned from Saccharum spp. hybrid cultivar ROC22. Real-time quantitative PCR (RT-qPCR) analysis demonstrated that these three ShCOI1 genes had divergent expression profiles in response to salicylic acid, abscisic acid, polyethylene glycol, cold, and S. scitamineum. Conclusions These results suggest that COI1 genes may act in sugarcane growth, development, and response to various stresses via different regulatory mechanisms, which laying a foundation for the functional identification of the sugarcane COI1 gene.

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Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses

Cited by 6 publications

References 31 publications

Function and Mechanism of Jasmonic Acid in Plant Responses to Abiotic and Biotic Stresses

Function and Mechanism of Jasmonic Acid in Plant Responses to Abiotic and Biotic Stresses

PlWRKY13: A Transcription Factor Involved in Abiotic and Biotic Stress Responses in Paeonia lactiflora

Genome-wide identification and expression analysis of the coronatine-insensitive 1 (COI1) gene family in response to biotic and abiotic stresses in Saccharum

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