Silencing Asian Seabass gab3 Inhibits Nervous Necrosis Virus Replication

Yang, Zituo; Yu, Yang; Wang, Le; Wong, Sek-Man; Yue, Gen Hua

doi:10.1007/s10126-022-10169-1

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…In Asian sea bass ( Lates calcarifer ), the gab3 susceptibility gene for nervous necrosis virus was detected. gab3 plays an important role in virus replication, and its suppression leads to lower virus titers in infected fish and increased survival during infection [ 340 ]. A susceptibility gene for grass carp reovirus has been found in grass carp [ 25 ].…”

Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

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Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

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Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

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show abstract

“…In addition to integrating exogenous AMGs into the genome, experiments have demonstrated that knockout of IRGEs can enhance resistance against pathogens by negatively regulating gene expression or disrupting pathways. For instance, a few representative genes, like rhamnose‐binding lectin ( RBL ), signal transducer and activator of transcription 2, junctional adhesion molecule‐A, the repressor of RNA polymerase III transcription of Paralichthys olivaceus ( PoMaf1 ), and GRB2‐associated binding protein 3 have undergone induced mutations that mimicked and altered the immunity of the fish and improved the host's resistance to disease 13,82 . Indeed, RBL is a critical component of fish's innate immunity as an antibacterial and non‐self‐recognition molecule, 83,84 especially in the protection of teleost eggs as well as in the mucosa 85 .…”

Section: Future Perspectivesmentioning

confidence: 99%

“…For instance, a few representative genes, like rhamnose-binding lectin (RBL), signal transducer and activator of transcription 2, junctional adhesion molecule-A, the repressor of RNA polymerase III transcription of Paralichthys olivaceus (PoMaf1), and GRB2-associated binding protein 3 have undergone induced mutations that mimicked and altered the immunity of the fish and improved the host's resistance to disease. 13,82 Indeed, RBL is a critical component of fish's innate immunity as an antibacterial and nonself-recognition molecule, 83,84 especially in the protection of teleost eggs as well as in the mucosa. 85 Interestingly, potential negative regulation of RBL is involved in the immunity of some fishes against pathogenic invasion.…”

Section: Transgenic Amgs and Fish Immune Systemmentioning

confidence: 99%

Enhancing aquaculture disease resistance: Antimicrobial peptides and gene editing

Wang

Cheng

2023

Reviews in Aquaculture

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Previous studies have demonstrated that CRISPR/Cas9‐mediated genome editing and transgenesis by integrating vector‐engineered antimicrobial peptide genes (AMGs) are effective to modulate the fish's innate immune system. To generalise the knowledge of AMG application in aquaculture, we recruited 544 data entries from a pool of empirical studies, which included 23 studies (two unpublished from our team) spanning 12 diseases. We systematically re‐processed and re‐analysed these data by harnessing a cross‐disease meta‐analysis. On aggregate, AMG‐genetic engineering aimed at enhancing disease resistance was shown to decrease the number of colony‐forming units of bacteria from fish tissues, increase post‐infection survival rates, and alter the expression of AMGs and immune‐related genes. Further, AMG pathogen‐combating activity was triggered within 2 h after infection and lasted 48 h. The overexpression of AMGs was highest in the spleen and skin, followed by the kidney and liver during this period. Typically, regardless of the type of AMGs, the synergistic expression of AMGs with IL‐10, IKβ, TGFβ, C3b and TLR genes in AMG‐integrated fish contributed to activating inflammatory/immune responses against pathogens. Our findings revealed that the efficiency of transgenic AMGs against pathogens was fish‐, pathogen‐ and AMG‐specific. Compared to bacteria, transgenic AMGs were less inhibitory to viruses and parasites. In addition, innovative CRISPR/Cas9‐based transgenesis enabling the site‐directed knock‐in of foreign genes at multiple loci was assessed for the enhancement of disease resistance in combination with other favourable fish‐production traits, including fast growth, sterility and enriched fatty‐acid content. Altogether, our findings indicated that AMGs as transgenes have substantial promise to modulate the fish's innate immune system and enhance disease resistance.

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Tbx21 gene and its association with resistance against viral nervous necrosis (VNN) in Asian seabass, Lates calcarifer

Wong,

Yang,

Wang

et al. 2024

Mar Life Sci Technol

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Silencing Asian Seabass gab3 Inhibits Nervous Necrosis Virus Replication

Cited by 6 publications

References 30 publications

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Enhancing aquaculture disease resistance: Antimicrobial peptides and gene editing

Tbx21 gene and its association with resistance against viral nervous necrosis (VNN) in Asian seabass, Lates calcarifer

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