Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose

Abstract

The lncRNA small nucleolar RNA host gene 1 (SNHG1) is a cerebral infarctionassociated gene, its biological role and mechanism in diabetic retinopathy remain to be illuminated. The present study was designed to investigate the role of SNHG1 in high glucose induced human retinal pigment epithelial cells (ARPE-19). Methods: ARPE-19 cells were cultured and exposed to 60 mM high glucose for 48h, and 5.5mM glucose-exposed ARPE-19 cells were used as the control. The levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin, ZO-1, vimentin and α-SMA were measured, and the Cell inflammatory response was evaluated by detecting IL-6 and IL-1β levels. Then, cell migration, proliferation and apoptosis were detected. The expression of the lncRNA SNHG1 in ARPE-19 cells was detected by quantitative real-time PCR. SNHG1 was knocked down by small interfering RNA (siRNA) transfection. The effects of SNHG1 inhibition on inflammation, EMT, migration, proliferation and apoptosis were observed. Results: The results showed that the expression of SNHG1 was significantly increased in ARPE-19 cells exposed to high glucose. Silencing SNHG1 reduced the expression of vimentin, α-SMA, and the expression of inflammatory chemokines IL-6 and IL-1β, inhibited migration and proliferation, elevated the expression of E-cadherin and ZO-1, and promoted apoptosis in ARPE-19 cells. Conclusion:The lncRNA SNHG1 is involved in hyperglycemia-induced EMT and the inflammatory response of ARPE-19 cells and provides a new understanding of the pathogenesis of DR.

Diabetic retinopathy: Involved cells, biomarkers, and treatments

“…HG levels can promote the migration and proliferation of RPE cells, reducing the expression of epithelial markers E-cadherin and ZO-1 and increasing the levels of mesenchymal markers vimentin and α-SMA, indicating EMT ( Yang J. et al, 2021 ). A recent study showed that knockdown of miR-195 can inhibit EMT and RPE cell permeability ( Fu S. H. et al, 2021 ).…”

Lipopolysaccharide enhances HSV-1 replication and inflammatory factor release in the ARPE-19 cells

“…However, ARPE-19 cells are not immortal, heterogeneous and have an unrepresentative transcriptome ( Pfeffer and Fliesler, 2022 ). In present studies, ARPE-19 cells were used to observe epithelial-mesenchymal transition, oxidative stress, high glucose-induced injury and inflammation response ( Qian et al., 2019 ; Ravi et al., 2021 ; Yang et al., 2021 ; Yuan et al., 2020 ). Therefore, ARPE-19 cells were selected to evaluate the viral replication and subsequent inflammatory response in this research.…”

Potential epigenetic molecular regulatory networks in ocular neovascularization

“…In the DR, the lncRNA small nucleolar RNA host gene 1 (SNHG1) is abnormally expressed (Yang et al, 2021). SNHG1 enhances vascular endothelial cell proliferation and angiogenesis in TNF-stimulated HUVEC (Zhang et al, 2020a).SNHG1-mediated histone changes are also implicated in neovascularization.…”