Silage studies. III. some characteristics of the silage microflora

Nilsson, Per Eric

doi:10.1007/bf00693106

Cited by 18 publications

(5 citation statements)

References 3 publications

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“…LAB commonly found on fresh herbage and in silage have been investigated [26, 27]. Nilsson and Nilsson [28] were found that the predominant LAB during silage fermentation were streptococci and lactobacilli, with Lactobacillus plantarum the species most frequently recovered. Other studies [29, 30] reported the occurrence of pediococci and lactobacilli on leaving or decayed plants.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Some Lactic Acid Bacteria Isolated from Bee Pollen: A Preliminary Study

Belhadj

Harzallah

Bouamra

et al. 2014

Bioscience of Microbiota, Food and Health

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In the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. All isolates were screened for their antagonistic activity against both Gram-positive and Gram-negative pathogenic bacteria. Neutralized supernatants of 54 lactic acid bacteria (LAB) cultures from 216 active isolates inhibited the growth of indicator bacteria. They were phenotypically characterized, based on the fermentation of 39 carbohydrates. Using the simple matching coefficient and unweighted pair group algorithm with arithmetic averages (UPGMA), seven clusters with other two members were defined at the 79% similarity level. The following species were characterized: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Pediococcus acidilactici, Pediococcus pentosaceus, and unidentified lactobacilli. Phenotypic characteristics of major and minor clusters were also identified. Partial sequencing of the 16S rRNA gene of representative isolates from each cluster was performed, and ten strains were assigned to seven species: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus ingluviei, Pediococcus pentosaceus, Lactobacillus acidipiscis and Weissella cibaria. The molecular method used failed to determine the exact taxonomic status of BH0900 and AH3133.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Some Lactic Acid Bacteria Isolated from Bee Pollen: A Preliminary Study

Belhadj

Harzallah

Bouamra

et al. 2014

Bioscience of Microbiota, Food and Health

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“…The characterization and isolation of the bacteria predominating during silage fermentation have been studied extensively (Stirling & Whittenbury 1963;Whittenbury 1968). Nilson (1956) found that the predominant lactic acid bacteria during fermentation are streptococci and lactobacilli, with Lacr. plantarum often being the most frequently recovered.…”

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confidence: 99%

A survey of lactic acid bacteria in Italian silage

Grazia

Suzzi

1984

Journal of Applied Bacteriology

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Grazia, L. & Suzzi, G. 1984. A survey of lactic acid bacteria in Italian silage. Journal of Applied Bacteriology56, 373–379. Lactic acid bacteria, isolated from Italian ensiled products, were represented by strains of the genera Lactobacillus and Leuconostoc. The predominant strains were heterofermentative lactobacilli, with Lactobacillus buchneri being the most frequent. Among homofermentative lactic acid bacteria, strains of Lact. plantarum and Lact. casei were recovered. Almost all strains utilized malic acid and showed good acid‐tolerance, but only some of them were able to metabolize malic acid at extremely low pH; these were five homofermentative lactobacilli (4 Lact. plantarum and 1 Lacr. casei var. casei) and two heterofermentative lactobacilli (Lact. cellobiosus and Lactobacillus sp.).

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“…Short lists of species and subspecies of lactic acid bacteria commonly found on fresh herbage and in silage have been published by Weise (1973), Woolford (1975) andMcDonald (1976). Nilsson (1956) found that the predominant lactic acid bacteria during fermentation were streptococci and lactobacilli, with Lact. plantarurn the species most frequently recovered.…”

Section: Discussionmentioning

confidence: 71%

“…Studies in the USA (Kroulik et al 1955) and in Sweden (Nilsson & Nilsson 1956) have also shown that multiplication of lactic acid bacteria occurs during wilting and harvesting. Conversely Beck (1978), in Germany, in a summary of studies on the microbiological status of green fodder plants before ensiling, points out that the number of lactobacilli is often less than 1OO/g and can in fact be reduced further during wilting.…”

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confidence: 99%

An investigation into the sources of lactic acid bacteria in grass silage

Fenton¹

1987

Journal of Applied Bacteriology

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During the 1983 silage making season an investigation was carried out to determine the contribution made by farm machinery to the numbers of lactic acid bacteria present on grass. Although no lactic acid bacteria were isolated from the standing crop, up to 103/g were found in the grass as it entered the clamp. Examination of machinery in close contact with the grass revealed lactic acid bacteria on the mower blades and on the forks of the rough‐terrain vehicle used to pack the grass into the clamp. During the 1984 season the occurrence of lactic acid bacteria in particular batches of grass from the standing crop through to silage after 2 d in the clamp was studied. There was a gradual increase in numbers of lactic acid bacteria in the grass during the process of mowing and collection. Wilting resulted in a greater initial increase in numbers but non‐wilted silage had the highest count after 48 h. The majority of organisms isolated from machinery before silage making were streptococci. After silage making the number and proportions of organisms changed, with numbers increasing in all cases, particularly from the mower with lactobacilli and streptococci predominating which indicates that passage through the machinery served to inoculate the grass.

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Silage studies. III. some characteristics of the silage microflora

Cited by 18 publications

References 3 publications

Phenotypic and Genotypic Characterization of Some Lactic Acid Bacteria Isolated from Bee Pollen: A Preliminary Study

Phenotypic and Genotypic Characterization of Some Lactic Acid Bacteria Isolated from Bee Pollen: A Preliminary Study

A survey of lactic acid bacteria in Italian silage

An investigation into the sources of lactic acid bacteria in grass silage

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