Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase

Abstract

In order to improve the silage fermentation of stylo (Stylosanthes guianensis) in tropical areas, stylo silages were prepared with commercial additives Lactobacillus plantarum Chikuso-1 (CH1), L. rhamnasus Snow Lact L (SN), Acremonium cellulase (CE) and their combination as SN+CE or CH1 + CE, and the fermentation quality, chemical composition and ruminal degradation of these silages were studied. Stylo silages treated with lactic acid bacteria (LAB) or cellulase, the pH value and NH -N ⁄ total-N were significantly (P < 0.05) decreased while the ruminal degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (aNDFom) and acid detergent fiber (ADFom) were significantly (P < 0.05) increased compared to control. Compared to LAB or cellulase-treated silages, the DM, CP contents and relative feed value (RFV), and the ruminal degradability in LAB plus cellulase-treated silages were significantly (P < 0.05) higher, but the aNDFom content was significantly (P < 0.05) lower. CH1 + CE treatment was more effective in silage fermentation and ruminal degradation than SN+CE treatment. The results confirmed that LAB or LAB plus cellulase treatment could improve the fermentation quality, chemical composition and ruminal degradation of stylo silage. Moreover, the combined treatment with LAB and cellulase may have beneficial synergistic effects on ruminal degradation.

Effects of fibrolytic enzymes and lactic acid bacteria on fermentation quality andin vitrodigestibility of Napier grass silage

“…Therefore, the addition of ENZ may not have beneficial effects on promoting the propagation of LAB. Previous studies, have suggested that addition of cellulase could improve the fermentation quality by degrading NDF and ADF of tropical crop and by-product silages (Khota et al 2016;Li et al 2017;Wang et al 2019). This result was not evident in our study, this could be attributed to the fibrolytic enzyme activity which depends on the temperature and pH condition (Colombatto et al 2004).…”

Changes of bioactive compounds in barley industry by‐products during submerged and solid state fermentation with antimicrobial Pediococcus acidilactici strain LUHS29

“…The experimental results are in agreement with Li, Zhou, Zi, and Cai (2017) who determined that LAB can improve fermentation quality, chemical composition, and bioavailability (inhibiting protein degradation and promoting fiber degradation), thus having great potential as an additive for bran fermentation. The experimental results are in agreement with Li, Zhou, Zi, and Cai (2017) who determined that LAB can improve fermentation quality, chemical composition, and bioavailability (inhibiting protein degradation and promoting fiber degradation), thus having great potential as an additive for bran fermentation.…”

Silage fermentation, chemical composition and ruminal degradation of king grass, cassava foliage and their mixture

“…Most of gramineae grasses were low in nutritional value as they have high moisture and fiber content but low protein content. In contrary, legume and foliage normally have low moisture and high protein content (Li, Zhou, Pan et al, ; Li, Zhou, Zi et al, ; Syahniar, Ridla, Jayanegara, & Samsudin, ). Their mixtures as silage have good fermentation quality and balanced nutrients.…”