“…Relatively, in the two stage operation, high methane yields were reached through the optimized first-stage approach. Methane produced up to 68%, similarly to that found by previous authors [25,26,27]. The same authors [26], reported also methane yields, for various kinds of substrates and the results of the present work were also coherent with previous experience by Tenca et al obtained with the same substrate [28,29,30].…”