SIK1-LNC represses the proliferative, migrative, and invasive abilities of lung cancer cells

Abstract

BackgroundDiscussions regarding the correlations between long non-coding RNAs (lncRNAs) and cancers have dominated research in recent years. SIK1-LNC, a type of lncRNA and adjacent to salt-inducible kinases 1 (SIK1), has been found aberrantly expressed in lung cancer. However, its functional role in lung cancer remains largely unknown.PurposeIn this study, we aimed to explore the association between SIK1-LNC expression and SIK1 in lung cancer cells and further identify the impact of SIK1-LNC on the proliferation, migration invasion of lung cancer cells.Patients and methodsOf the 30 patients with non-small-cell lung carcinoma from Zhongnan Hospital of Wuhan University, RT-qPCR was performed to detect SIK1 and SIK1-LNC expressions in patients’ samples. Overexpression and knockdown experiments were conducted to analyze the SIK1 and SIK1-LNC expressions in lung cancer cell lines. CCK-8, Brdu, scratch wound-healing, and Transwell assays were respectively employed to evaluate the proliferative, migrative, and invasive abilities of lung cancer cells.ResultsBoth SIK1-LNC and SIK1 expression levels were evidently downregulated in 30 lung cancer tissues. SIK1-LNC expression was bound up with clinicopathologic features, including lymph node metastasis and distant metastasis. SIK1 expression showed a positive tendency with SIK1-LNC expression in lung cancer cells. SIK1-LNC exerted a significant repression on cell proliferatiive, miogrative and invasive abilities of lung cancer cells.ConclusionOur findings suggested that SIK1-LNC may act as a novel biomarker and therapeutic target for lung cancer.

Comprehensive analysis of differentially expressed long non‑coding RNAs in non‑small cell lung cancer

“…Previous studies have demonstrated that lncRNAs serve a prominent role in the progression of NSCLC, as either oncogenes or tumor suppressors; lncRNA-MALAT-1 enhanced NSCLC cell motility (25) and lncRNA-CCAT2 promoted the invasion of NSCLC cells (26). However, lncRNA-SIK1-LNC suppressed the proliferative and invasive abilities of NSCLC cells (27). lncRNA-PICART1 suppressed the proliferation of NSCLC cells by inhibiting JAK2/signal transducer and activator of transcription 3 signaling (28).…”

Exosomal miR-130b-3p targets SIK1 to inhibit medulloblastoma tumorigenesis

“…Recent evidence has shown that SIK1 performs an important role in human cancers, such as non-small-cell lung cancer 42 , epithelial ovarian cancer 43 or hepatocellular carcinoma 41,44 . Previous research has shown that SIK1 is a tumor suppressor in many cancers 45,46 . Interestingly, in the current study, we identified SIK1 as a tumor-promoting protein, as it can induce tumor cell proliferation, invasion and metastasis in vivo/vitro.…”

The potent roles of salt-inducible kinases (SIKs) in metabolic homeostasis and tumorigenesis

“…143 In contrast, lncRNA TCONS_00029157, also termed SIK1-LNC, was positively associated with SIK1 expression, and they together inhibited lung cancer cell malignant phenotypes. 144 …”