Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma

Jia, Jianguang; Qin, Yiyu; Zhang, Ligong; Guo, Chenxu; Wang, Yaguo; Yue, Xicheng; Qian, Jun

doi:10.3892/etm.2017.5560

“…Rats in each group were given gavage for 7 days. There were a large number of researches [11][12][13][14] using drug-containing serum to study the mechanism of their protective effects. Based on the human equivalent dose in the clinical practice and the animals' surface area, we could covert human dose to the rat dose [15].…”

Section: Herbal Decoction Preparationmentioning

confidence: 99%

Qiangji Jianli Decoction Alleviates Hydrogen Peroxide‐Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts

Song

¹

,

Li

²

,

Ke

³

et al. 2021

Oxidative Medicine and Cellular Longevity

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Oxidative stress can cause the excessive generation of reactive oxygen species (ROS) and has various adverse effects on muscular mitochondria. Qiangji Jianli decoction (QJJLD) is an effective traditional Chinese medicine (TCM) that is widely applied to improve muscle weakness, and it has active constituents that prevent mitochondrial dysfunction. To investigate the protective mechanism of QJJLD against hydrogen peroxide- (H2O2-) mediated mitochondrial dysfunction in L6 myoblasts. Cell viability was determined with MTT assay. Mitochondrial ultrastructure was detected by transmission electron microscope (TEM). ROS and mitochondrial membrane potential (MMP) were analyzed by fluorescence microscope and flow cytometry. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) level were determined by WST-1, TBA, and DTNB methods, respectively. The mRNA and protein levels were measured by quantitative real-time PCR (qRT-PCR) and Western blot. The cell viability was decreased, and the cellular ROS level was increased when L6 myoblasts were exposed to H2O2. After treatment with QJJLD-containing serum, the SOD and GSH-Px activities were increased. MDA level was decreased concurrently. ROS level was decreased while respiratory chain complex activity and ATP content were increased in L6 myoblasts. MMP loss was attenuated. Mitochondrial ultrastructure was also improved. Simultaneously, the protein expressions of p-AMPK, PGC-1α, NRF1, and TFAM were upregulated. The mRNA and protein expressions of Mfn1/2 and Opa1 were also upregulated while Drp1 and Fis1 were downregulated. These results suggest that QJJLD may alleviate mitochondrial dysfunction through the regulation of mitochondrial dynamics and biogenesis, the inhibition of ROS generation, and the promotion of mitochondrial energy metabolism.

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“…SJZ decoction could improve the immune function and QOL. What's more, SZJ decoction could maintain peripheral blood leukocyte count and peritoneal macrophage activity after chemotherapy; in addition, it could regulate the immune function of the body and enhance the anticancer effect of immune cells [19][20][21][22]. SJZ decoction could inhibit gastric cancer cell growth in vivo, and the mechanism was associated with the downregulation of the expression of p53 mRNA [23].…”

Section: Introductionmentioning

confidence: 99%

Enteral Nutrition Combined with Improved-Sijunzi Decoction Shows Positive Effect in Precachexia Cancer Patients: A Retrospective Analysis

Li

¹

,

Chen

²

,

Zeng

³

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Cancer has been considered as the leading cause of death in the world. In patients with cancer, up to 80% display a cachectic period after diagnosis. Cachexia is known to have a negative impact on function, treatment tolerance, higher rates of hospitalizations, and mortality. Anorexia is often used as a warning sign of precachexia. Long-term anorexia may lead to malnutrition and, then, accelerate the occurrence of cachexia. A safe and effective treatment, which can both improve appetite and assist nutritional support for precachexia cancer patients shows its particular important role. Methods. A retrospective analysis comparing the different therapeutic effects on precachexia cancer patients with anorexia-malnutrition. We recorded 46 patients with the improved-Sijunzi decoction combined with enteral nutrition emulsion (ISJZ group) and 35 patients with single enteral nutrition emulsion (SEN group). The different therapeutic effects of the two groups were observed by recording indicators before and 2 weeks after treatment, including patient-generated subjective global assessment score, quality of life score, Karnofsky performance status scale, Eastern cooperative oncology group scale standard and traditional Chinese medicine syndrome, daily total dietary intake, red blood cells, hemoglobin, prealbumin, albumin, total protein cholinesterase, C-reactive protein, leukocytes, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea nitrogen, and creatinine. Results. ISJZ group exhibited prominent improvement of traditional Chinese medicine syndrome (TCMS), nutritional condition, and quality of life compared with the SEN group (QOL: p = 0.0001 , PG-SGA: p = 0.019 , dietary intake: p = 0.0001 , TCMS: p = 0.0001 ). The levels of HGB ( p = 0.006 ), PAlb ( p = 0.001 ), Alb ( p = 0.0001 ), TP ( p = 0.008 ), and ChE ( p = 0.0001 ) in the ISJZ group were higher than the SEN group after treatment. Moreover, the ratios of CRP/ALB ( p = 0.028 ) and CRP/PALB ( p = 0.005 ) in the two groups have obvious differences; they were lower for the ISJZ group than the SEN group. Conclusions. Enteral nutrition combined with ISJZ decoction is an effective treatment in precachexia cancer patients for the prevention of cachexia. This treatment therapy can alleviate the inflammatory response, improve malnutrition state, and promote the performance status. Tianjin Medical University Cancer Institute and Hospital approved this study (Trial No. 1913).

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“…The Sijunzi decoction (SJZD) is a classic Chinese formula for “reinforcing asthenia qi” and nourishing the spleen; it consists of Renshen (Codonopsis pilosula), Baizhu (Atractylodes macrocephala), Fulin (Poria cocos), and Gancao ( Glycyrrhiza uralensis) [ 13 ]. In clinical applications, researchers have recently observed that the SJZD has some therapeutic effects in the treatment of malignant digestive tumors [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

A Network Pharmacology Approach for Uncovering the Antitumor Effects and Potential Mechanisms of the Sijunzi Decoction for the Treatment of Gastric Cancer

Ding

¹

,

Guo

²

,

Wang

³

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Sijunzi decoction (SJZD), a classic Chinese formula, has been clinically used for the treatment of gastrointestinal disorders. However, few studies have uncovered its antitumor effects and its potential mechanisms against gastric cancer (GC). Therefore, this work aimed to identify the active compounds and putative targets of the SJZD and to further explore the potential mechanisms involved in the treatment of GC. Materials and Methods. The active compounds and potential targets of the SJZD and related genes for GC treatment were collected from a public database. Traditional Chinese medicine (TCM)-compound-target-disease networks, Venn diagrams, protein–protein interactions (PPIs), gene ontology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to obtain the bioactive compounds, key targets, and potential pathways. Next, the human gastric adenocarcinoma cell line NUGC-4 was inoculated subcutaneously into the right flank of NCG mice to build a tumor-bearing mouse model to further verify the findings. Results. There were 117 compounds in the SJZD in total. The SJZD and GC had 161 and 3288 potential targets, respectively, among which 123 targets overlapped. The network analysis showed that quercetin, kaempferol formononetin, ginsenoside, atractylenolide III, etc., were bioactive molecules. The tumor necrosis factor (TNF), interleukin-6 (IL-6), cellular tumor antigen p53 (TP53), transcription factor AP-1 (JUN), and vascular endothelial growth factor A (VEGFA) were potential targets. A KEGG pathway enrichment analysis revealed 110 pathways involved in the pathways for cancer, including the PI3K-AKT signaling pathway. Validation experiments showed that the SJZD inhibited tumor growth and induced apoptosis in tumor cells. In addition, the SJZD downregulated expressions of VEGFA, iNOS, COX-2, and Bax/Bcl2 and inhibited the expressions of p-PI3K and p-AKT. Conclusion. The SJZD treats GC by inhibiting blood vessel hyperplasia and inducing cell apoptosis by regulating the PI3K/AKT pathway.

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Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma

Cited by 11 publications

References 39 publications

Qiangji Jianli Decoction Alleviates Hydrogen Peroxide‐Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts

Qiangji Jianli Decoction Alleviates Hydrogen Peroxide‐Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts

Enteral Nutrition Combined with Improved-Sijunzi Decoction Shows Positive Effect in Precachexia Cancer Patients: A Retrospective Analysis

A Network Pharmacology Approach for Uncovering the Antitumor Effects and Potential Mechanisms of the Sijunzi Decoction for the Treatment of Gastric Cancer

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