SiiE Is Secreted by the
            <i>Salmonella enterica</i>
            Serovar Typhimurium Pathogenicity Island 4-Encoded Secretion System and Contributes to Intestinal Colonization in Cattle

Morgan, Elfed; Bowen, Alison J.; Carnell, Sonya; Wallis, Timothy S.; Stevens, Mark P.

doi:10.1128/iai.01438-06

Cited by 78 publications

(74 citation statements)

References 33 publications

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“…Our previous analyses in a mouse model of intestinal inflammation indicate that SPI1, as well a SPI4, contribute to the inflammatory response of the mucosa (14). A further study by Morgan et al (28) showed a role in colonization of the bovine intestine. On the cellular level, SPI4 mediates binding to the apical surfaces of polarized epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

“…We have recently determined that SPI4 encodes a type I secretion system (T1SS) and SiiE, a large repetitive protein. SiiE is the substrate of the SPI4 T1SS and functions as a nonfimbrial adhesin in binding to epithelial cell surfaces (14,28). Previous studies identified SPI4 as a factor for the intestinal colonization of calves (29), as well as for establishing persistent infections in the mouse model (22).…”

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confidence: 99%

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Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica

et al. 2007

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Salmonella pathogenicity island 4 (SPI4) encodes a type I secretion system and the cognate substrate protein, SiiE. We have recently demonstrated that SiiE is a giant nonfimbrial adhesin involved in the adhesion of Salmonella enterica serovar Typhimurium to polarized epithelial cells. We also observed that under in vitro culture conditions, the synthesis and secretion of SiiE coincided with the activation of Salmonella invasion genes. These observations prompted us to investigate the regulation of SPI4 genes in detail. A novel approach for the generation of reporter gene fusions was employed to generate single-copy chromosomal fusions to various genes within SPI4, and the expression of these fusions was investigated. We analyzed the regulation of SPI4 genes and the roles of various regulatory systems for SPI4 expression. Our data show that the expression of SPI4 genes is coregulated with SPI1 invasion genes by the global regulator SirA. Expression of a SPI4 gene was also reduced in the absence of HilA, the central local regulator of SPI1 gene expression. Both SirA and HilA functions were required for the secretion of SiiE and the SPI4-mediated adhesion. Our data demonstrate that SPI4-mediated adhesion, as well as SPI1-mediated invasion, are tightly coregulated by the same regulatory circuits and induced under similar environmental conditions.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica

et al. 2007

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“…For example, multiple attenuating mutations were identified in SPI-4 in calves, but not chickens or pigs challenged with the same library (Morgan et al 2004;Carnell et al 2007). SPI-4 encodes a Type I protein secretion system that secretes a ca 595 kDa protein (SiiE; Morgan et al 2007) that mediates adherence (Gerlach et al 2007) and invasion of bovine ileal mucosa , possibly by working in concert with T3SS-1 (Gerlach et al 2008). It is unlikely that SPI-4 acts in a fully bovine-specific manner, as SPI-4 mutants exhibited reduced virulence following oral inoculation of mice (Morgan et al 2004;Gerlach et al 2007).…”

Section: Molecular Basis Of Intestinal Colonization Of Food-producingmentioning

confidence: 99%

Molecular insights into farm animal and zoonoticSalmonellainfections

Stevens

Humphrey

Maskell

2009

Phil. Trans. R. Soc. B

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142

102

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Salmonella enterica is a facultative intracellular pathogen of worldwide importance. Infections may present in a variety of ways, from asymptomatic colonization to inflammatory diarrhoea or typhoid fever depending on serovar-and host-specific factors. Human diarrhoeal infections are frequently acquired via the food chain and farm environment by virtue of the ability of selected non-typhoidal serovars to colonize the intestines of food-producing animals and contaminate the avian reproductive tract and egg. Colonization of reservoir hosts often occurs in the absence of clinical symptoms; however, some S. enterica serovars threaten animal health owing to their ability to cause acute enteritis or translocate from the intestines to other organs causing fever, septicaemia and abortion. Despite the availability of complete genome sequences of isolates representing several serovars, the molecular mechanisms underlying Salmonella colonization, pathogenesis and transmission in reservoir hosts remain ill-defined. Here we review current knowledge of the bacterial factors influencing colonization of food-producing animals by Salmonella and the basis of host range, differential virulence and zoonotic potential.

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“…Di zi ana li zi ça-lış ma la rın dan el de edi len ve ri ler, söz ko nu su böl ge nin S. typhi ile S. typhi mu ri um ara sın da önem li fark lı lık lar gös ter di ği ne işa ret et miş tir. 9,63 SPI-5, sal gı sis tem le ri ile trans lo kas yon la rı gerçek leş ti ri len efek tör pro te in le ri kod la mak ta dır. T3SS-1 ara cı lı ğı ile trans lo kas yo nu ya pı lan ve sı vı sa lı nı mı nı baş la ta rak di ya re ye ne den olan SopB efek tör pro te i ni ve T3SS-2 ara cı ğı ile trans lo kas yonu ger çek leş ti ri len PipB efek tör pro te i ni bu lo kusta kod lan mak ta dır.…”

Section: Clinical Microbiologyunclassified

Molecular Mechanisms of Salmonella Pathogenicity: Review

Akçelik¹,

Akkoç²

2010

Turkiye Klinikleri J Med Sci

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SiiE Is Secreted by the Salmonella enterica Serovar Typhimurium Pathogenicity Island 4-Encoded Secretion System and Contributes to Intestinal Colonization in Cattle

Cited by 78 publications

References 33 publications

Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica

Salmonella Pathogenicity Island 4-Mediated Adhesion Is Coregulated with Invasion Genes in Salmonella enterica

Molecular insights into farm animal and zoonoticSalmonellainfections

Molecular Mechanisms of Salmonella Pathogenicity: Review

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