Signs of atopic dermatitis and contact dermatitis affected by distinct H2-haplotype in the NC/Nga genetic background

Abstract

We recently advocated in favour of naming a novel H2-haplotype consisting of Kd, D/Ldm7, I-Ak and I-Ek in the atopic dermatitis (AD) mouse model NC/Nga as “H-2nc.” The role of the H2-haplotype in AD development was investigated in H2b-congenic NC/Nga mice (NC.h2b/b and NC.h2b/nc) established by backcrossing. A severe 2,4-dinitrofluorobenzene (DNFB)-induced dermatitis in NC/Nga was alleviated partially in NC.h2b/nc and significantly in NC.h2b/b. The AD phenotype was correlated with thymic stromal lymphopoietin (TSLP)-epidermal expression levels and serum levels of total IgE and IL-18/IL-33. Histologically, allergic contact dermatitis (ACD) was accompanied by lymphocytes and plasma cells-infiltrating perivasculitis in NC.h2b/nc and NC.h2b/b and clearly differed from AD accompanied by neutrophils, eosinophils and macrophages-infiltrating diffuse suppurative dermatitis in NC/Nga. Interestingly, IFN-γ/IL-17 production from autoreactive CD4+ T-cells remarkably increased in DNFB-sensitised NC.h2b/b but not in NC/Nga. Our findings suggest that AD or ACD may depend on haplotype H-2nc or H-2b, respectively, in addition to the NC/Nga genetic background.

Ghrelin protects against contact dermatitis and psoriasiform skin inflammation by antagonizing TNF-α/NF-κB signaling pathways

“…However, the mechanisms and cure for contact dermatitis and psoriasis remain unknown 4,57–59 . Many cell types, including epidermal cells 60 , lymphocytes 61 , macrophages 62 , fibroblasts 63 and dendritic cells 64 , secrete ghrelin, and these cells have been reported to mediate effects on skin inflammation, including contact dermatitis and psoriasis 65–67 , indicating the possibility that ghrelin may affect contact dermatitis and psoriasis. However, the effectiveness and mechanism of ghrelin on these skin inflammatory diseases have not been explored.…”

Urinary lipid profile of atopic dermatitis in murine model and human patients

“…The DNFB-induced skin inflammation mice model was generated as described previously. 10 Briefly, 3 days after depilatory the dorsal hair, the mice were sensitized on day 1 by applying 25 µl 0.5% DNFB (w/v) in a mixture of acetone and olive oil (4:1) on the upper side of dehaired back skin. On days 5, 8, and 11, the mice were challenged with 100 µl vehicle or 0.2% DNFB on lower side of back skin.…”

Mechanisms of unconventional CD8 Tc2 lymphocyte induction in allergic contact dermatitis: Role of H3/H4 histamine receptors

“…Characteristically, Langerhans cells internalize the hapten-protein complex, starting their migration at the lymph node to initiate an adaptive response. The effector response is associated with the induction of a Thelper immune response (5)(6)(7). It should be noted that nonconventional CD8 lymphocytes can be categorized into different subtypes called Tc2, Tc17, and Tc9 depending on the profile of cytokines secreted (Th2, Th17, or Th9, respectively).…”