Significantly inhibitory effects of low molecular weight heparin (Fraxiparine) on the motility of lung cancer cells and its related mechanism

Zhong, Guofang; Gong, Yi; Chen, Yu; Wu, Shifei; Ma, Qingping; Wang, Yu; Ren, Jiang; Zhang, Xue-Chao; Yang, Wenxuan; Zhu, Wen

doi:10.1007/s13277-015-3117-8

Cited by 20 publications

(13 citation statements)

References 56 publications

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“…Previously, Nadroparin has been suggested to reduce the invasive, migratory, and adhesive ability of human lung adenocarcinoma A549 cells by down-regulating the expression of integrin β1 and β3 as well as matrix MMP2 and 9 and by restraining the actin cytoskeleton reorganization [47]. In our study, MMP2 (and MMP9 for LNCaP) were produced by cell tumors in inactive forms and Apixaban did not activate the MMPs produced by cancer cells.…”

Section: Discussionmentioning

confidence: 48%

In vitro effects of Apixaban on 5 different cancer cell lines

et al. 2017

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BackgroundCancer is associated with hypercoagulability. However, several data suggest that anticoagulant drugs may have an effect on tumor development and progression mediated by both coagulation dependent processes and non-coagulation dependent processes. Therefore, we investigated the in vitro effects of Apixaban on cell proliferation, mortality, cell migration, gene expression and matrix metalloproteinase in 5 different cancer cell lines.MethodsThe following cancer cell lines, and 2 normal fibroblast cultures (lung and dermal fibroblasts), were studied: OVCAR3 (ovarian cancer), MDA MB 231 (breast cancer), CaCO-2 (colon cancer), LNCaP (prostate cancer) and U937 (histiocytic lymphoma). Proliferation and cell mortality were assessed in control cells and Apixaban treated cultures (dose from 0.1 to 5 μg/ml, 0 to 96-h). Necrosis/Apoptosis (fluorescence microscopy), cell migration (24-h after scratch test), matrix metalloproteinase (MMP) activity and mRNA expression (RT PCR) of p16, p21, p53 and HAS were also assessed.ResultsHigh-dose (5 μg/ml) Apixaban incubation was associated with a significantly reduced proliferation in 3 cancer cell lines (OVCAR3, CaCO-2 and LNCaP) and with increased cancer cell mortality in all, except LNCaP, cancer lines. Apoptosis seems to account for the increased mortality. The migration capacity seems to be impaired after high-dose Apixaban incubation in OVCAR3 and CaCO-2 cells. Data on mRNA expression suggest a consistent increase in tumor suppression gene p16 in all cell lines.ConclusionsOur data suggest that high-dose Apixaban may be able to interfere with cancer cell in vitro, reducing proliferation and increasing cancer cell mortality through apoptosis in several cancer cell lines.

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Section: Discussionmentioning

confidence: 48%

In vitro effects of Apixaban on 5 different cancer cell lines

et al. 2017

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“…Furthermore, the present study demonstrated the inhibitory effect of ELE on cell invasion and migration using three cellular models of metastasis. ELE inhibited the migratory and invasive ability of 4T1 cells, and the inhibitory rates were comparable to those of LMWH, which is a potential inhibitor of cancer metastasis (25,26). …”

Section: Discussionmentioning

confidence: 73%

Elemene inhibits the migration and invasion of 4T1 murine breast cancer cells via heparanase

Zhang

Sun

Nan

et al. 2017

Molecular Medicine Reports

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Elemene (ELE), a natural plant drug extracted from Curcumae Rhizoma, has been widely used for cancer treatment in China for more than 20 years. Although it is reported to be a broad-spectrum anticancer drug, the mechanism underlying the action of ELE in the treatment of breast cancer remains to be fully elucidated. Heparanase, a mammalian endo-D-glucuronidase, is involved in degradation of the extracellular matrix (ECM), and thus promotes tumor progression and metastasis. The downregulation of heparanase can effectively reduce tumor malignant behaviors. In the present study, the inhibitory effects of ELE were evaluated in breast cancer cells using a Cell Counting kit 8 assay. The migratory and invasive capabilities of cancer cells were investigated using a wound healing assay, real-time cell analysis and a Transwell assay. In addition, western blot analysis was used to assess alterations in the expression levels of key proteins. The present results confirmed the antiproliferative and antimetastatic effects of ELE, using low-molecular weight heparin (LMWH) as a positive control. In addition, ELE was demonstrated to downregulate the expression of heparanase, and decrease the phosphorylation of extracellular signal-regulated kinase and AKT. These findings suggested that ELE may be a promising agent targeting heparanase in the treatment of breast cancer.

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“…Adequate fluid supplementation is necessary for maintaining sufficient vital organ perfusion for the brain, coronary artery, and the kidney [ 4 ]. LMWH therapy was reported to inhibit plasma thrombin generation [ 36 ] and can also inhibit chemotactic migration of cancer cells [ 37 ]. Chemokines are also related to tumor progression and metastasis [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

The Role of Pulmonary Veins in Cancer Progression from a Computed Tomography Viewpoint

Liaw

Chang

Liao

et al. 2016

Journal of Oncology

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Background. We studied the role of pulmonary veins in cancer progression using computed tomography (CT) scans. Methods. We obtained data from 260 patients with pulmonary vein obstruction syndrome (PVOS). We used CT scans to investigate pulmonary lesions in relation to pulmonary veins. We divided the lesions into central and peripheral lesions by their anatomical location: in the lung parenchymal tissue or pulmonary vein; in the superior or inferior pulmonary vein; and by unilateral or bilateral presence in the lungs. Results. Of the 260 PVOS patients, 226 (87%) had central lesions, 231 (89%) had peripheral lesions, and 190 (75%) had mixed central and peripheral lesions. Among the 226 central lesions, 93% had lesions within the superior pulmonary vein, either bilaterally or unilaterally. Among the 231 peripheral lesions, 65% involved bilateral lungs, 70% involved lesions within the inferior pulmonary veins, and 23% had obvious metastatic extensions into the left atrium. All patients exhibited nodules within their pulmonary veins. The predeath status included respiratory failure (40%) and loss of consciousness (60%). Conclusion. CT scans play an important role in following tumor progression within pulmonary veins. Besides respiratory distress, PVOS cancer cells entering centrally can result in cardiac and cerebral events and loss of consciousness or can metastasize peripherally from the pulmonary veins to the lungs.

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Significantly inhibitory effects of low molecular weight heparin (Fraxiparine) on the motility of lung cancer cells and its related mechanism

Cited by 20 publications

References 56 publications

In vitro effects of Apixaban on 5 different cancer cell lines

In vitro effects of Apixaban on 5 different cancer cell lines

Elemene inhibits the migration and invasion of 4T1 murine breast cancer cells via heparanase

The Role of Pulmonary Veins in Cancer Progression from a Computed Tomography Viewpoint

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