Significantly Improved Analytical Sensitivity of Lateral Flow Immunoassays by Using Thermal Contrast

Qin, Zhenpeng; Chan, Warren C. W.; Boulware, David R.; Akkin, Taner; Butler, Eric; Bischof, John C.

doi:10.1002/anie.201200997

Cited by 165 publications

(124 citation statements)

References 36 publications

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“…Then, light excitation can produce a thermal signal, which can be detected by simple visual inspection on a thermosensitive support or by a thermal camera. 236 The sensitivity, which can be up to attomolar range in serum of patients, 45 and the simplicity of this method are astonishing. The principles and protocol of this novel approach are schematically represented in Fig.…”

Section: Figmentioning

confidence: 96%

“…Indeed, CNTs and Au NPs have been widely used as transducers in potentiometric analysis. [236][237][238] As in the different sensing methods discussed so far, the most important concept here is that NPs are functionalized with catching molecules, which, upon recognition, change the properties of the transducers. For instance, an aptamer-based CNT potentiometric sensor has been used to detect ultralow concentrations of bacteria.…”

Section: Electric Readoutmentioning

confidence: 99%

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Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Pino¹

2014

J. Biomed. Opt

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Abstract. Continuous advances in the field of bionanotechnology, particularly in the areas of synthesis and functionalization of colloidal inorganic nanoparticles with novel physicochemical properties, allow the development of innovative and/or enhanced approaches for medical solutions. Many of the present and future applications of bionanotechnology rely on the ability of nanoparticles to efficiently interact with electromagnetic (EM) fields and subsequently to produce a response via scattering or absorption of the interacting field. The crosssections of nanoparticles are typically orders of magnitude larger than organic molecules, which provide the means for manipulating EM fields and, thereby, enable applications in therapy (e.g., photothermal therapy, hyperthermia, drug release, etc.), sensing (e.g., surface plasmon resonance, surface-enhanced Raman, energy transfer, etc.), and imaging (e.g., magnetic resonance, optoacoustic, photothermal, etc.). Herein, an overview of the most relevant parameters and promising applications of EM-active nanoparticles for applications in life science are discussed with a view toward tailoring the interaction of nanoparticles with EM fields. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Figmentioning

confidence: 96%

Section: Electric Readoutmentioning

confidence: 99%

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Pino¹

2014

J. Biomed. Opt

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“…25 However, the existing LFAs are associated with the limitation of poor sensitivity. 11,26 Several methods have been developed to enhance the sensitivity of LFAs, such as sample concentration, [27][28][29][30] thermal contrast, 31 fluidic control, [32][33][34] temperature-humidity technique, 35 probe-based signal enhancement, 23,36 enzyme-based signal amplification 37,38 and electrochemical devices-based enhancement. 39 However, these approaches require either external equipment, 35 high-cost reagents 23,37 or complicated fabrication with multistep procedure, 32 limiting their application for POC testing.…”

Section: Introductionmentioning

confidence: 99%

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Gong¹,

Hu²,

Choi³

et al. 2017

IJN

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Heart failure (HF) has become a major cause of morbidity and mortality with a significant global economic burden. Although well-established clinical tests could provide early diagnosis, access to these tests is limited in developing countries, where a relatively higher incidence of HF is present. This has prompted an urgent need for developing a cost-effective, rapid and robust diagnostic tool for point-of-care (POC) detection of HF. Lateral flow immunoassay (LFIA) has found widespread applications in POC diagnostics. However, the low sensitivity of LFIA limits its ability to detect important HF biomarkers (e.g., brain natriuretic peptide [BNP]) that are normally present in low concentration in blood. To address this issue, we developed an improved LFIA by optimizing the gold nanoparticle (GNP)–antibody conjugate conditions (e.g., the conjugate pH and the amount of added antibody), the diameter of GNP and the concentration of antibody embedded on the test line and modifying the structure of test strip. Through these improvements, the proposed test strip enabled the detection of BNP down to 0.1 ng/mL within 10–15 min, presenting ~15-fold sensitivity enhancement over conventional lateral flow assay. We also successfully applied our LFIA in the analysis of BNP in human serum samples, highlighting its potential use for clinical assessment of HF. The developed LFIA for BNP could rapidly rule out HF with the naked eye, offering tremendous potential for POC test and personalized medicine.

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“…(5) Others have employed methods involving dual-gold nanoparticle conjugates (3) or thermal contrast enhancement that requires a laser and an infrared temperature gun. (4) In contrast, the enhancement that we describe is advantageous, as it does not require any extra reagents or equipment. Additionally, while a two-step assay may be cumbersome in point-of-care situations, one benefit of decreasing the GNP quantity is the marked reduction in assay cost.…”

Section: Lateral Flow Immunoassay With Different Running Methodsmentioning

confidence: 97%

“…(1) However, achieving low analytical sensitivity with a visual colorimetric signal is one of the limitations of these devices. For sandwich lateral flow immunoassays that use a hapten and antibody detection system, the analytical sensitivity is reported to be improved through the use of enzyme substrates, (2) dual-gold nanoparticle conjugates, (3) thermal contrast, (4) and changes to the device architecture. (5) While each of these methods improved the signal intensity and analytical sensitivity, they also increased the complexity of the detection system.…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Signal of Lateral Flow Immunoassays by Using Different Developing Methods

2015

Sensors and Materials

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Lateral flow immunoassay devices are potential biosensors for point-of-care diagnostics. However, these devices are limited by low analytical sensitivity when coupled with a visual colorimetric signal. Here, we analyzed key parameters related to nucleic acid lateral flow performance and related this to the analytical theory behind lateral flow functionality. In particular, we predicted a set of different physical running methods to optimize the assay signal intensity by ensuring higher binding of the signal molecules to the DNA. We found that a double-run method improved the assay signal intensity on average by approximately 50%, and a fully absorbed conjugate pad method came close to the double-run signal efficacy at high DNA concentrations. Our results demonstrate that even when the signal molecule is supplied in excess, a significant proportion of analytes bind to the detection antibody in a colorless complex. The lowest detection limits achieved were 0.1 picomole for detection using an anti-Cy5 antibody and 0.01 picomole for the detection using an anti-Texas Red antibody. Our double-run method improvement is generic, does not require additional reagents and equipment, reduces assay costs compared to the fully absorbed method, and is applicable to other lateral flow immunoassays.

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Significantly Improved Analytical Sensitivity of Lateral Flow Immunoassays by Using Thermal Contrast

Cited by 165 publications

References 36 publications

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Enhancing the Signal of Lateral Flow Immunoassays by Using Different Developing Methods

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