Significantly enhanced bioconversion of high titer biomass-derived furfural to furfuryl alcohol by robust endogenous aldehyde reductase in a sustainable way

Di, Junhua; Liao, Xiaolong; Li, Qi; He, Yu-Cai; Ma, Cuiluan

doi:10.1039/d3gc01417g

Cited by 7 publications

(1 citation statement)

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“…The approach of coupling reductase with dehydrogenase for coenzyme regeneration is an efficient way to improve the bioreduction efficiency [74][75][76]. The asymmetric biosynthesis of (R)-CHBE from COBE was conducted through the bioreduction with CgCR cells containing CgCR and GDH in the presence of glucose.…”

Section: The Effect Of Cell Permeability and Damage Degree On Biocata...mentioning

confidence: 99%

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Yang,

Xu,

Jiang

et al. 2023

Processes

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Objective: Optically active (R)-ethyl 4-chloro-3-hydroxybutyrate ((R)-CHBE) is a useful chiral building block for the synthesis of pharmaceuticals. Recently, there has been great interest in the synthesis of (R)-CHBE via the highly stereoselective bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE) under mild conditions. Methods: A highly efficient bioreduction process for transforming COBE into (R)-CHBE was developed in a biocompatible organic solvent–deep eutectic solvent–water reaction medium. Results: Recombinant Escherichia coli containing carbonyl reductase (CgCR) and glucose dehydrogenase (GDH) was successfully constructed and characterized. In addition, the feasibility of the asymmetric bioreduction of COBE to (R)-CHBE was verified in an organic solvent–deep eutectic solvent–water (ethyl acetate-betaine/lactic acid-water) system. At pH 7.0 and 30 °C, the kinetic constants Km and kcat of COBE were 20.9 mM and 56.1 s−1, respectively. A high (R)-CHBE yield (≥90%) was achieved by catalyzing COBE (1000 mM) in 12 h with E. coli CgCR cells in the presence of Ni2+ (7 mM) and glucose (3.5 mM glucose/mM COBE) in an ethyl acetate-betaine/lactic acid-H2O (50/7/43, v/v/v) system. The effects of organic solvents and DESs on whole-cell permeability were analyzed. Conclusions: An efficient bioreduction system was constructed for biologically transforming COBE to (R)-CHBE via whole-cell biocatalysis, and the established bioprocess has potential application in future.

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Section: The Effect Of Cell Permeability and Damage Degree On Biocata...mentioning

confidence: 99%

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Yang,

Xu,

Jiang

et al. 2023

Processes

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Chemobiocatalytic transformation of biomass-derived D-xylose into furfuryl alcohol in a sustainable reaction system

Yang,

Liu,

Peng

et al. 2023

Catalysis Communications

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Organic-inorganic hybrid materials for catalytic transfer hydrogenation of biomass-derived carbonyl-containing compounds

Wu,

Bai,

Deng

et al. 2025

Coordination Chemistry Reviews

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Significantly enhanced bioconversion of high titer biomass-derived furfural to furfuryl alcohol by robust endogenous aldehyde reductase in a sustainable way

Abstract: To date, a highly efficient manufacture of valuable biofurans from abundant, available and renewable bioresources has attracted much attentions in a sustainable way. The major aim of this study was...

Cited by 7 publications

References 59 publications

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Asymmetric Bioreduction of Ethyl 4-Chloroacetoacetate into Ethyl 4-Chloro-3-hydroxybutyrate by Recombinant Escherichia coli CgCR in Ethyl Acetate-Betaine:Lactic Acid-Water

Chemobiocatalytic transformation of biomass-derived D-xylose into furfuryl alcohol in a sustainable reaction system

Organic-inorganic hybrid materials for catalytic transfer hydrogenation of biomass-derived carbonyl-containing compounds

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