Significant metabolic improvement by a water extract of olives: animal and human evidence

Peroulis, Nikolaos; Androutsopoulos, Vasilis P.; Notas, George; Koinaki, Stella; Giakoumaki, Elsa; Spyros, Apostolos; Manolopoulou, Εfstathia; Kargaki, Sophia; Tzardi, Maria; Moustou, Eleni; Stephanou, Euripides G.; Bakogeorgou, Efstathia; Malliaraki, Niki; Niniraki, Maria; Lionis, Christos; Castanas, Elias; Kampa, Marilena

doi:10.1007/s00394-018-1807-x

Cited by 18 publications

(17 citation statements)

References 74 publications

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“…A proprietary method has been used to recover part of the wastewater through filtration and purification for further use. The chemical characterization of these OMWW extracts revealed that they are largely enriched in soluble polyphenols [17]. Recovering polyphenols from waste material is an approach that we have now established, and the ultra-purified extracts from OMWWs that we termed A009 [10,15,16] have been tested for effects on cancer cells.…”

Section: Introductionmentioning

confidence: 99%

An Extract of Olive Mill Wastewater Downregulates Growth, Adhesion and Invasion Pathways in Lung Cancer Cells: Involvement of CXCR4

et al. 2020

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Several diet-derived compounds have been reported to exert antioxidant, anti-proliferative and anti-angiogenic effects in numerous cancers and could be beneficial in cancer prevention. Olive oil production involves the generation of an aqueous phase defined as olive mill wastewater (OMWW), a polluting effluent rich in soluble polyphenols. Here, we assessed the cancer preventive properties exerted by a purified extract of OMWW (A009) for its activity on lung cancer cell lines. Hydroxytyrosol, the most abundant polyphenol present in our A009 extracts, was used as reference molecule in the assays performed. Extracts from OMWW from two different olive oil cultivars were used. We found that the A009 extracts limit lung cancer cell proliferation in a dose and time dependent manner. These effects were associated with the induction of apoptosis. A009 extracts were effective in inhibiting adhesion capabilities on a fibronectin layer accompanied with a reduction in their ability to generate invasive sprouts in a Matrigel layer. The production of chemokine CXCL12 and CXCR4 receptor were reduced by treatment with the extracts. Also, A009 interfered with the production of proangiogenic and pro-inflammatory VEGF, CXCL8, and CCL2 (as detected by FACS analysis) in the lung cell lines. A009 extracts were able to decrease STAT3 phosphorylation in lung cancer cells. Our results show that A009 extracts reduced activities related to tumor cell behavior in lung cancer cell lines, suggesting that they could have a potential cancer preventive role.

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Section: Introductionmentioning

confidence: 99%

An Extract of Olive Mill Wastewater Downregulates Growth, Adhesion and Invasion Pathways in Lung Cancer Cells: Involvement of CXCR4

et al. 2020

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“…Large volumes of wastewater (olive mill wastewater; OMWW) are generated during olive oil production, particularly during the malaxation process (continuous washing of olive paste with warm water prior to the procedure of separation of the oil from the solid fraction [15,16]). OMWWs are rich in polyphenols associated with beneficial effects, that include the metabolic compartment [17]. We previously reported that a polyphenol-rich purified extract from OMWW, termed A009, which is particularly rich in hydroxytyrosol (HyT), determines anti-angiogenic effects both in endothelial cells in vitro and in an angiogenesis model in vivo [18].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater

Baci

Gallazzi

Cascini

et al. 2019

IJMS

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Dietary phytochemicals are particularly attractive for chemoprevention and are able to modulate several signal transduction pathways linked with cancer. Olive oil, a major component of the Mediterranean diet, is an abundant source of phenolic compounds. Olive oil production is associated with the generation of a waste material, termed ‘olive mill wastewater’ (OMWW) that have been reported to contain water-soluble polyphenols. Prostate cancer (PCa) is considered as an ideal cancer type for chemopreventive approaches, due to its wide incidence but relatively long latency period and progression time. Here, we investigated activities associated with potential preventive properties of a polyphenol-rich olive mill wastewater extract, OMWW (A009), on three in vitro models of PCa. A009 was able to inhibit PCa cell proliferation, adhesion, migration, and invasion. Molecularly, we found that A009 targeted NF-κB and reduced pro-angiogenic growth factor, VEGF, CXCL8, and CXCL12 production. IL-6/STAT3 axis was also regulated by the extract. A009 shows promising properties, and purified hydroxytyrosol (HyT), the major polyphenol component of A009, was also active but not always as effective as A009. Finally, our results support the idea of repositioning a food waste-derived material for nutraceutical employment, with environmental and industrial cost management benefits.

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“…In this sense, 50 mL of VOO during three weeks reduced oxLDL and lipid-peroxides levels in 40 males with cardiovascular heart disease [63]. Furthermore, the long-term consumption of a polyphenol-rich water extract of olives, which was generated during OO production, also reduced oxLDL in healthy young men [64]. Another long-term intervention of functional VOO enriched with PCs from thyme and OO (FVOOT; 500 ppm) reduced the LDL-P/HDL-P ratio.…”

Section: Effects Of Voo and Phenol-enriched Voos On Ldl Atherogenicitymentioning

confidence: 94%

Effects of Virgin Olive Oil and Phenol-Enriched Virgin Olive Oils on Lipoprotein Atherogenicity

et al. 2020

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The atherogenicity of low-density lipoprotein (LDL) and triglyceride-rich lipoproteins (TRLs) may be more significant than LDL cholesterol levels. Clinical trials which have led to increased high-density lipoprotein (HDL) cholesterol have not always seen reductions in cardiovascular disease (CVD). Furthermore, genetic variants predisposing individuals to high HDL cholesterol are not associated with a lower risk of suffering a coronary event, and therefore HDL functionality is considered to be the most relevant aspect. Virgin olive oil (VOO) is thought to play a protective role against CVD. This review describes the effects of VOO and phenol-enriched VOOs on lipoprotein atherogenicity and HDL atheroprotective properties. The studies have demonstrated a decrease in LDL atherogenicity and an increase in the HDL-mediated macrophage cholesterol efflux capacity, HDL antioxidant activity, and HDL anti-inflammatory characteristics after various VOO interventions. Moreover, the expression of cholesterol efflux-related genes was enhanced after exposure to phenol-enriched VOOs in both post-prandial and sustained trials. Improvements in HDL antioxidant properties were also observed after VOO and phenol-enriched VOO interventions. Furthermore, some studies have demonstrated improved characteristics of TRL atherogenicity under postprandial conditions after VOO intake. Large-scale, long-term randomized clinical trials, and Mendelian analyses which assess the lipoprotein state and properties, are required to confirm these results.

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Significant metabolic improvement by a water extract of olives: animal and human evidence

Abstract: Our data clearly show that OLWPE can improve glucose and lipid profile, indicating its possible use in the design of functional food and/or therapeutic interventions.

Cited by 18 publications

References 74 publications

An Extract of Olive Mill Wastewater Downregulates Growth, Adhesion and Invasion Pathways in Lung Cancer Cells: Involvement of CXCR4

An Extract of Olive Mill Wastewater Downregulates Growth, Adhesion and Invasion Pathways in Lung Cancer Cells: Involvement of CXCR4

Downregulation of Pro-Inflammatory and Pro-Angiogenic Pathways in Prostate Cancer Cells by a Polyphenol-Rich Extract from Olive Mill Wastewater

Effects of Virgin Olive Oil and Phenol-Enriched Virgin Olive Oils on Lipoprotein Atherogenicity

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