Significant differences in fecal microbiota are associated with various stages of glucose tolerance in African American male veterans

Abstract

The importance of gut microbiota in pathogenesis of diabetes remains unknown. This study investigated the relationship between microbiota and metabolic markers in African American men (AAM) with prediabetes and hypovitaminosis D. The study was ancillary to a randomized trial of vitamin D supplementation with weekly ergocalciferol (50,000 IU) conducted in AAM veterans over 12 months (D Intervention in Veterans Affairs). Glycemic groups (Gr) were characterized based on changes in oral glucose tolerance between baseline and exit. Subjects with stable normal glucose tolerance were assigned to Gr-1 and those with stable prediabetes (impaired glucose tolerance and impaired fasting glucose) to Gr-2. Microbiota composition was analyzed in stool collected at the exit (n = 115) and compared between Gr-1 and Gr-2, as well as between the lowest and highest quartiles of dietary intake of energy and fat, hemoglobin A1c, and serum 25-hydroxyvitamin D (25[OH]D) level. Differences between Gr-1 and Gr-2 included the Bacteroidetes/Firmicutes and Bacteroidales/Clostridia ratios and differences in genera such as Ruminococcus and Dialister. Changes in specific taxa associated with the lowest and highest quartiles of 25(OH) D (eg, Ruminococcus, Roseburia, Blautia, Dorea) were clearly distinct from those of dietary intake (eg, Bacteroides, Bacteroides/Prevotella ratio) or A1c (eg, Faecalibacterium, Catenibacterium, Streptococcus). These findings suggest a novel interaction between microbiota and vitamin D and a role for microbiota in early stages of diabetes development. Although results suggest that specific taxa are associated with glycemic stability over time, a causative relationship between microbiota makeup and dysglycemia is still to be demonstrated.

The Gut Microbial Diversity of Newly Diagnosed Diabetics but Not of Prediabetics Is Significantly Different from That of Healthy Nondiabetics

“…Metformin treatment commonly prescribed for diabetes has also been linked with higher levels of Akkermansia in diabetic patients (39) due to enhancement of mucin-producing goblet cells (40). We did not find any change in the abundance of Ruminococcus in PreDMs, in contrast to the report of Ciubotaru et al (41). Additionally, we observed decreased abundance of Prevotella, Blautia, and Ruminococcus and increased abundance of Lactobacillus in NewDMs.…”

The endocrine vitamin D system in the gut

“…In a double-blind, placebo-controlled, randomized, parallel-arm, multicenter study, oral supplementation with probiotic Lactobacillus reuteri NCIMB 30242 increased circulating 25(OH)D levels relative to placebo (Jones et al, 2013). Notably, in a study in African American males, the level of circulating 25(OH)D was associated with differences in stool microbial composition: a decrease in the relative abundance of Roseburia, Blautia, Ruminococcus and Dorea (Firmicutes phylum, Clostridia class) was found in individuals with higher 25(OH)D concentration (Ciubotaru et al, 2015). Clearly, much work is needed to define the crosstalk between the vitamin D system and intestinal microbiota.…”

Dietary Assessment Methods in Military and Veteran Populations: A Scoping Review

“…There was one (1%) of each study design for longitudinal cohort [21], validation study [71], cohort study [88], and retrospective cross-sectional study [29]. Twenty-one of the studies were interventions including eight (9%) randomised control trials [7,36,39,51,53,89,94,103], four (4%) pre-…”