Significant correlation between peripheral blood <scp>CD</scp>34+ cell count in children prior to aphaeresis and <scp>CD</scp>34+ cell yield following aphaeresis: A single‐center experience

Rosenfeld-Keidar, Hila; Eshel, Rinat; Pinhasov, Albert; Bitan, Menachem; Edelman, Sabina; Broitman, Marcela; Dvir, Rina; Sadot, Efraim; Levin, Dror; Manisterski, Michal; Berger-Achituv, Sivan; Elhasid, Ronit

doi:10.1111/petr.13150

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…In our study, we confirmed well‐known positive correlation between mobilized peripheral CD34+ cell count with CD34+ yield 17–19 . We have collected targeted 5 × 10 6 cells/kg by first‐day apheresis in more than 60% of patients with peripheral CD34+ cell count >10/μL.…”

Section: Discussionsupporting

confidence: 86%

“…In our study, we confirmed well-known positive correlation between mobilized peripheral CD34+ cell count with CD34+ yield. [17][18][19] We have collected targeted 5 × 10 6 cells/kg by first-day apheresis in more than 60% of patients with peripheral CD34+ cell count >10/ μL. Rujkijyanont et al 18 retrospectively studied the largest pediatric series with 396 apheresis procedures in 301 pediatric cancer patients and reported higher pre-apheresis peripheral CD34+ levels of >35 × 10 6 CD34+ cells/μL were strong predictor value for stem cell collection.…”

Section: Discussionmentioning

confidence: 99%

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Autologous peripheral blood stem cell mobilization and apheresis in pediatric patients with cancer: A single‐center report of 64 procedures

Yazal Erdem,

Özyörük,

Ok Bozkaya

et al. 2024

Pediatric Transplantation

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BackgroundThe published experience concerning autologous peripheral blood stem cell collection in children is very limited.MethodsThe data of pediatric patients who underwent autologous stem cell mobilization and apheresis between January 2011 and April 2020 were analyzed retrospectively.ResultsWe studied retrospectively 64 mobilization and apheresis procedures in 48 pediatric patients (34 males, 14 females), mean age of 7.31 ± 5.38 (range, 1.5–19.7) years, the underlying disease was mostly neuroblastoma (NBL). The body weight of 21 patients (43.75%) was 15 kg or less. The targeted autologous peripheral stem cell apheresis (APSCA) was successfully achieved in 98% of patients. Neuroblastoma patients were younger than the rest of the patients and underwent apheresis after receiving fewer chemotherapy cycles than others and all of them mobilized within the first session successfully. Plerixafor was added to mobilization in nine heavily pretreated patients (18.7%), median two doses (range, 1–4 doses). 11 patients (22.9%) underwent radiotherapy (RT) before mobilization with doses of median 24 Gy (range, 10.8–54.0 Gy). Patients with RT were older at the time of apheresis and had received more chemotherapy courses than patients without RT. As a result, patients with a history of RT had significantly lower peripheral CD34+ cells and CD34+ yields than those without RT. In 17 patients (35.4%), 22 different complications were noted. The most common complications were catheter‐related infections (n:10, 20.8%), followed by catheter‐related thrombosis in eight patients (16.7%).ConclusionsPatients who had far less therapy before apheresis were more likely to mobilize successfully. Our study provides a detailed practice approach including complications during APSCA aiming to increase the success rates of apheresis in transplantation centers.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Autologous peripheral blood stem cell mobilization and apheresis in pediatric patients with cancer: A single‐center report of 64 procedures

Yazal Erdem,

Özyörük,

Ok Bozkaya

et al. 2024

Pediatric Transplantation

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Specific Features of the Hematopoietic Cell Mobilization, Collection and Transplantation in Children With Malignant Diseases

Avramova

2024

Comprehensive Hematology and Stem Cell Research

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Optimized peripheral blood progenitor cell mobilization for autologous hematopoietic cell transplantation in children with high‐risk and refractory malignancies

Furlong

Jensen

Woodard

et al. 2019

Pediatric Transplantation

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BackgroundAutologous hematopoietic stem cell transplantation (aHSCT) using hematopoietic progenitor cells (HPCs) has become an important therapeutic modality for patients with high‐risk malignancies. Current literature on standardized method for HPC apheresis in children is sparse and failure rate reported as high as 30%.Patients/MethodsA retrospective study of 125 pediatric patients with high‐risk malignancies undergoing aHSCT in Western Australia between 1997 and 2016 was conducted.ResultsMobilization was achieved by means of chemotherapy and granulocyte colony‐stimulating factor (G‐CSF). Patients underwent apheresis the day after CD34+ counts reached ≥20/µL and an additional dose of G‐CSF. Peripheral arterial and intravenous lines were inserted in pediatric intensive care unit under local anesthetic and/or sedation, omitting the need for general anesthesia as well as facilitating an uninterrupted apheresis flow. Larger apheresis total blood volumes were processed in patients weighing ≤20 kg. The minimal dose of ≥2 × 106 CD34+ cells/kg was successfully collected in 98.4% of all patients. The optimal dose of 3‐5 × 106 CD34+ cells/kg was collected in 96% of patients scheduled for a single aHSCT, 87.5% for tandem, and 100% for triple aHSCT. All HPC collections were completed in one apheresis session. Mobilization after ≤3 chemotherapy cycles and cycles including cyclophosphamide resulted in a significantly higher yield of CD34+ cells.ConclusionOur approach to HPC mobilization by means of chemotherapy and single myeloid growth factor combined with optimal collection timing facilitated by continuous apheresis flow resulted in highly effective HPC harvest in children and adolescents with high‐risk cancers.

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Significant correlation between peripheral blood CD34+ cell count in children prior to aphaeresis and CD34+ cell yield following aphaeresis: A single‐center experience

Cited by 3 publications

References 24 publications

Autologous peripheral blood stem cell mobilization and apheresis in pediatric patients with cancer: A single‐center report of 64 procedures

Autologous peripheral blood stem cell mobilization and apheresis in pediatric patients with cancer: A single‐center report of 64 procedures

Specific Features of the Hematopoietic Cell Mobilization, Collection and Transplantation in Children With Malignant Diseases

Optimized peripheral blood progenitor cell mobilization for autologous hematopoietic cell transplantation in children with high‐risk and refractory malignancies

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